肺癌患者红细胞促外周血淋巴细胞增殖能力的实验研究

目的探讨肺癌患者红细胞(RBC)促外周血淋巴细胞(PBL)增殖能力,了解肺癌患者RBC免疫调控功能状况.方法肺癌患者(检测组)和正常人(对照组))的RBC为效应细胞,正常人淋巴细胞为靶细胞,采用四甲基偶氮唑蓝(MTT)比色法,检测红细胞调控淋巴细胞增殖反应的能力.结果健康人RBC促PBL增殖作用明显,其增殖率为(57.3±10.2)%;而肺癌患者RBC促PBL增殖作用较健康人明显低下,增殖率为(38.4±8.6)%(P＜0.01);肺癌患者手术后RBC促PBL增殖作用有明显改善,增殖率为(43.9±11.4)%,仍低于健康人.结论肺癌患者红细胞免疫调控功能低下.     

HSPA13在ARPE19细胞氧化应激中的作用

目的 探讨HSPA13在ARPE19细胞氧化应激中的作用。 方法 采用CCK8法检测不同浓度H2O2对ARPE19细胞活力的影响;Western印迹法和免疫荧光检测H2O2对HSPA13表达的影响;CCK8法检测HSPA13的表达水平对氧化应激诱导的细胞活力的影响;过表达或siRNA干扰HSPA13后,分别检测氧化应激相关指标和促炎因子的分泌;RNA测序分析空载组和HSPA13干扰组差异表达的基因,并进行GO富集及KEGG信号通路分析。 结果 CCK8法结果显示,与H2O2空白对照组相比,100、200、300、400μmol/L H2O2处理组细胞活力降低（P<0.05）。Western印迹法结果显示,与H2O2空白对照组相比,H2O2处理组HSPA13的表达上升（P<0.05）。CCK8法结果显示,过表达HSPA13可保护细胞活力。氧化应激相关指标和促炎因子检测结果显示,与H2O2处理组相比,过表达HSPA13加H2O2处理组中SOD1和GPX1 mRNA表达上升,MDA含量降低,GSH含量上升,IL-8、IL-2和IL-1β分泌下降;而siRNA干扰HSPA13加H2O2处理组的结果则相反,并且活性氧类含量上升（P<0.05）。RNA测序结果显示,与空载组相比,HSPA13干扰组差异基因表达上调的有64个,下调的有254个。GO富集分析表明,HSPA13与细胞代谢或多种生理过程相关。KEGG分析显示,HSPA13与PI3K-Akt信号通路、TGFβ信号通路相关。 结论 氧化应激诱导ARPE19细胞中HSPA13的表达上调;HSPA13在细胞氧化损伤中发挥保护作用,这为寻找年龄相关性黄斑变性新的治疗靶点提供有益的线索。     

红参发酵产物对高糖下大鼠肾小球系膜细胞增殖和细胞外基质降解的影响

目的：探讨红参发酵产物（FRGE）对高糖刺激下大鼠肾小球系膜细胞（GMCs）增殖和细胞外基质（ECM）降解的影响,阐明FRGE对糖尿病肾病（DN）的防治作用及可能机制。方法：将GMCs分为正常对照（NG）组、高糖（HG）组、高糖＋不同浓度（3.75、7.50和15.00 mg·L^-1）FRGE组。四甲基偶氮唑盐（MTT）光吸收法观察各组GMCs的增殖率,酶联免疫吸附法（ELISA）检测培养细胞上清中Ⅳ型胶原蛋白（Col Ⅳ）的水平,蛋白免疫印迹技术（Western blotting）检测基质金属蛋白酶2（MMP-2）和基质金属蛋白酶组织抑制物2（TIMP-2）蛋白表达水平。结果：与NG组比较,HG组GMCs增殖率升高（P<0.01）,Col Ⅳ水平升高（P<0.01）,TIMP-2蛋白表达水平上调（P<0.01）,MMP-2蛋白表达水平下调（P<0.01）;与HG组比较,高糖＋不同浓度FRGE组GMCs增殖率均降低（P<0.01）,Col Ⅳ水平降低（P<0.01）,TIMP-2蛋白表达水平下调（P<0.01）,MMP-2蛋白表达水平上调（P<0.01）。结论：FRGE可通过抑制高糖诱导的大鼠GMCs增殖,促进ECM的降解,延缓DN的发生发展。     

Q开关激光去除色素的组织学观察

目的：探讨新型Q开关激光去除皮肤细胞黑素的机理。方法：用Q532nm、Q755nm及Q1064nm三种Q开关激光治疗豚鼠的黑色皮肤区,观察治疗结果,并于激光治疗前、治疗后即刻、治疗后第3天、14天取材行光镜和电镜检查。结果：Q开关激光治疗后皮肤黑素颗粒即被气化或碎裂而减少或消失,黑素细胞呈空泡样变,细胞轮廓仍完整保留。治疗后第3天,表皮层明显增厚,细胞空泡样变逐渐好转,皮肤组织结构正常,皮下组织有少量淋巴细胞浸润和轻度充血,Q532nm组及Q755nm组表皮基底层及棘层仍有细小黑素颗粒,较正常黑色皮肤色素颗粒明显减少,Q755nm组黑素颗粒的密度明显少于Q532nm组,Q1064nm组表皮各层无明显黑素颗粒存在;治疗后第14天皮肤组织基本完全修复,Q532nm组及Q755nm组表皮基底层及棘层仍有细小黑素颗粒,以基底层较明显,密度较治疗后第3天加强,Q1064nm组表皮各层无细小黑素颗粒存在。结论：Q开关激光可选择性地破坏黑素颗粒,对含黑素颗粒的细胞无不可逆性损伤,Q1064nm激光治疗黑素颗粒效果最好,表明是目前治疗色素增加性皮肤病比较有效、副作用小的方法。     

Presence of Hepatitis E Virus in a RED Deer (Cervus elaphus) Population in Central Italy

Hepatitis E is an acute human disease caused by the hepatitis E virus (HEV). In addition to humans, HEV has been detected in several animal species and is recognized as a zoonotic pathogen. Pigs, wild boar and deer can be reservoir. In this study, we evaluated HEV prevalence in a free‐living red deer (Cervus elaphus) population in central Italy by detecting virus‐specific antibodies and RNA in sera. A total of 35 of 251 red deer sera were positive for anti‐HEV IgG. HEV RNA was detected in 10 of 91 sera examined. Two genomic fragments targeted by diagnostic PCRs in the capsid region were sequenced, both matching with genotype 3 HEV. Overall results confirmed the occurrence of HEV infection in deer also in Italy.     

Blood utilization in children managed non-operatively for blunt solid organ injury

BACKGROUND: Blood product utilization is an important issue in health care, given the frequent shortages in hospitals and the societal burden required to maintain the supply. Therefore, we retrospectively audited our blunt spleen/liver trauma experience to determine the percentage of cross-matched blood that was transfused to see whether more stringent typing criteria should be applied. METHODS: A retrospective analysis of a recent 7-year experience with nonoperative management in patients with blunt spleen or liver injury was performed. Demographics, packed red blood cells prepared by cross-match, and transfusions were measured. Unmatched, O-type blood given in the trauma bay was excluded. Patients undergoing laparotomy for solid organ injury were excluded. Data are expressed as mean +/- standard deviation. RESULTS: During the study period, 130 patients were nonoperatively managed for spleen and/or liver injury. Mean age was 8.7 +/- 4.6 years, and 62% were male. The mean grade of injury was 2.4 +/- 0.9. A total of 187 units of packed red blood cells was ordered in 60 patients. A total of 46.5 units was administered to 22 patients, revealing a 24.9% transfusion rate for the units ordered in 36.7% of the patients for whom it was ordered. When patients with other major injuries and those with ongoing bleeding requiring an operation or who clinically required blood on presentation were excluded, there were 80 patients. In this stable population, 104 units of PRBCs were ordered for 29 patients. A total of 18 units was then transfused in 5 patients, for a 17.3% transfusion rate for the units orders in 17.2% of the patients for whom it was ordered. None of the 5 patients received transfusion the day of admission. CONCLUSION: Hemodynamically stable patients with blunt spleen/liver injury triaged to conservative management should have their blood typed and be monitored closely for signs or laboratory values that would mandate a cross-match. According to our data, this strategy would safely improve utilization of blood bank resources.     

红蓝光治疗痤疮临床疗效观察

目的:探讨红蓝光照射治疗痤疮的临床疗效和安全性。方法:将113例轻、中度痤疮患者分为两组,实验组采用本院自制的克林霉素醇溶液联合红蓝光照射治疗,对照组仅外用本院自制的克林霉素醇溶液治疗。采用痤疮综合分级系统(GAGS)评价治疗前、后疗效。结果:实验组和对照组有效率分别为82.8%和43.6%,两组的治疗有效率存在显著性差异(P<0.01)。GAGS综合分值均较治疗前明显下降(P<0.01),但实验组下降更显著。结论:红蓝光治疗面部轻、中度痤疮安全有效。     

法夫酵母色素的组成与性质

对法夫酵母色素的组成及其光谱特性和稳定性进行了研究.结果表明法夫酵母色素是由10多种类胡萝卜素组成,其中虾青素为78%;色素呈亮丽的红色,对热较敏感,对自然光、紫外光、氧很敏感,色拉油和抗氧化剂对其有一定的保护作用.     

Determination of erythrocyte antioxidant capacity in haemodialysis patients using electron paramagnetic resonance.

BACKGROUND: The increased oxidative stress of uraemia is caused both by an increased generation of oxygen-free radicals and a decrease of antioxidative forces. There are, however, conflicting data concerning disturbances of the radical-scavenging power of red blood cells (RBCs) in uraemic patients. METHODS: The antioxidant capacities of the RBCs of 10 haemodialysis (HD) patients and 10 controls were examined after treatment with 0.324 mM tert-butylhydroperoxide (t-BOOH) in phosphate-buffered saline at 37 degrees C using electron paramagnetic resonance (EPR) with 5,5-dimethylpyrroline-N-oxide (DMPO) as a spin trap and glutathione (GSH) regeneration as an indicator of hexose monophosphate shunt (HMPS) activity. EPR investigations were also done after pre-incubation with N-ethylmaleimide (NEM) to inhibit the GSH system. Furthermore, we determined the RBC redox state in 15 HD patients and 15 controls. RESULTS: There was no difference between HD patients and controls in the elimination of t-BOOH-generated free radicals in the RBCs. A more than 20-fold increase in radical concentration was observed after GSH trapping with NEM. In this case, we found a delayed decrease of the relative radical concentration in HD patients compared with controls with a significant difference after 7 min (2.2+/-0.26 vs 1.60+/-0.21; P=0.005) and after 10 min (1.82+/-0.41 vs 0.83+/-0.44; P=0.001). GSH regeneration via HMPS did not differ between the RBCs of HD patients (99.5+/-13.5 nmol/min x ml RBC) and those of the controls (94.2+/-16.9 nmol/min x ml RBC). There were no differences in the RBC concentrations of GSH, GSSG, NADP, NADPH, and in the GSH/GSSG and NADP/NADPH ratios between HD patients and controls. CONCLUSIONS: These data suggest a strong antioxidant potential in the GSH system of erythrocytes without any evidence of a disturbance in HD patients. The HMPS pathway also appears not to be impaired in the RBCs of HD patients. However, the slower radical elimination in the RBCs of HD patients after inhibition of GSH-depending radical scavengers as compared with controls indicates a defect in the antioxidant forces outside the GSH system, and could be one reason for the reduced lifespan of RBCs in HD patients.