慢性鼻-鼻窦炎内镜手术前后OMC区分泌腺的观察研究

目的:观察慢性鼻窦炎(CS)鼻内镜鼻窦手术前后鼻黏膜黏液纤毛清除系统的分泌腺结构和功能变化。方法:对20例慢性鼻窦炎术前及术后4个月窦口鼻道复合体(OMC)区鼻黏膜进行AB-PAS染色,光镜下观察,并与健康组(对照组10例)进行对照。结果:鼻窦炎术前组黏膜怀状细胞及黏膜下腺体有显著性增加(P<0.01),型鼻窦炎患者病理性腺体的增生尤为明显。内镜术后4个月鼻黏膜杯状细胞可恢复至正常水平,与对照组比较差异无显著性(P>0.05),而黏膜下腺体未能恢复正常。结论:鼻黏膜杯状细胞及黏膜下腺体的增生是黏液纤毛清除功能障碍的原因之一,尤其是病理性腺体的增生。内镜术后4个月鼻黏膜上皮化,但黏液纤毛清除系统的恢复需要更长时间。术后随访时间应超过4个月,以防复发。     

ZDY102对拟痴呆大鼠脑M受体的影响

目的　观察知母活性成分ZMS的异构体ZDY10 2对拟痴呆大鼠脑M受体及学习记忆功能的影响。方法　大鼠单侧脑基底核部位定位注射 β 淀粉样肽 (2 5～ 35 )和鹅蕈膏酸造成拟痴呆模型 ,设立不同剂量ZDY10 2治疗组、模型组和对照组 (注射生理盐水 ) ,分别用Y 迷宫测定学习记忆能力及放射配基结合分析法测定注射同侧脑内M胆碱受体密度。结果　拟痴呆模型大鼠学习和记忆成绩和脑M受体密度明显低于对照组。连续两个月喂服ZDY10 2的模型大鼠学习和记忆能力显著好于对照组 ,脑M受体密度也显著提高 ,而且脑M受体密度与动物的学习记忆能力呈显著正相关。结论　ZDY10 2能明显改善拟痴呆大鼠的学习记忆能力 ,同时能显著提高脑内M受体密度 ,从两者的显著正相关来看 ,提高脑M受体密度可能是ZDY10 2改善拟痴呆模型学习记忆的重要机制。和以往对知母活性成分的研究结果相比较可以看出 ,2 5位甲基的立体异构对上述效果无明显影响     

六神丸HPLC-UV/ELSD指纹图谱研究

目的?建立六神丸的HPLC-UV/ELSD指纹图谱分析方法,对六神丸中主要特征峰进行归属并进行指认。方法?采用HPLC法,色谱柱为Kromasil C₁₈（250?mm×4.6?mm,5?μm）;乙腈-0.05%三氟乙酸水溶液作为流动相,进行梯度洗脱;紫外检测器（UV）参数:检测波长为296?nm;蒸发光散射检测器（ELSD）参数:漂移管温度为70?℃,喷雾器为冷却模式,氮气压力为25?psi;进样量20?μL;体积流量为1?mL/min。通过《中药色谱指纹图谱相似度评价系统（2012版）》,建立14批六神丸指纹图谱的共有模式,计算其相似度,并对共有峰进行药材的归属以及成分进行指认。结果?分别建立了六神丸HPLC-UV及HPLC-ELSD的指纹图谱,及其共有模式,指认出HPLC-UV指纹图谱共有峰14个,14批六神丸制剂相似度为0.940～0.988,指认出HPLC-ELSD指纹图谱共有峰13个,14批六神丸制剂相似度为0.968～0.998。确认了和蟾蜍他灵、沙蟾毒精、远华蟾毒精、蟾毒它灵、华蟾毒它灵、蟾毒灵、胆酸、鹅去氧胆酸、去氧胆酸。结论?首次建立了六神丸HPLC-UV/ELSD指纹图谱分析方法,该法操作准确、简单,重复性好、精密度高,可用于分析六神丸中化学成分信息,为六神丸质量控制提供了可靠的科学依据。      

基于主成分分析下慢性阻塞性肺疾病急性加重频率的判别分析

目的 对慢性阻塞性肺疾病患者（COPD)）一般资料及临床资料进行主成分分析和判别分析,研究影响疾病急性加重频率的因素,以预测患者后续发病频率。 方法 选取我院2016年1月～2017年10月收治的350例COPD患者为研究对象,收集患者例入院时一般资料、24h内动脉血气分析,首次降钙素原（PCT）含量、C反应蛋白（CRP）含量及血常规,及入院结束治疗时的抗生素治疗时间、住院时间。对入选患者随访1年,并明确1年内慢性阻塞性肺疾病急性加重（AECOPD）次数,根据疾病加重次数将患者分为三组（A组：≤1次;B组：＜3次;C组：≥3次）。比较三组一般资料及临床资料的差异,利用因子分析提取上述临床指标及一般资料主成分,并利用主成分对AECOPD患者急性加重频数行判别分析,比较提取主成分前后判别分析率差异。结果 共纳入患者321例,其中A组105例、B组105例、C组111例,三组一般资料及临床资料除吸烟年无明显差异,其余指标组间比较,差异有统计学意义（P＜0.05）。从临床指标及一般资料中共提取5个主成分,综合信息提取率833%。一般资料及临床指标判别分析提示,总体判别正确率为748%,A、B、C组三组正确判别率分别为88.6%、65.7%、70.3%;利用提取的主成分进行判别分析提示,总体判别正确率72.0%,A、B、C组三组正确判别率分别为971%、60%、595%。结论 利用常见一般资料及临床资料进行判别分析,可预测患者发病频数,主成分分析的判别正确率与综合资料判别正确率间无明显差异。     

Correcting waveform bias using principal component analysis: Applications in multicentre motion analysis studies

Multicentre studies are rare in three dimensional motion analyses due to challenges associated with combining waveform data from different centres. Principal component analysis (PCA) is a statistical technique that can be used to quantify variability in waveform data and identify group differences. A correction technique based on PCA is proposed that can be used in post processing to remove nuisance variation introduced by the differences between centres. Using this technique, the waveform bias that exists between the two datasets is corrected such that the means agree. No information is lost in the individual datasets, but the overall variability in the combined data is reduced. The correction is demonstrated on gait kinematics with synthesized crosstalk and on gait data from knee arthroplasty patients collected in two centres. The induced crosstalk was successfully removed from the knee joint angle data. In the second example, the removal of the nuisance variation due to the multicentre data collection allowed significant differences in implant type to be identified. This PCA-based technique can be used to correct for differences between waveform datasets in post processing and has the potential to enable multicentre motion analysis studies.     

食品中非法添加和滥用化学染料的分析研究

建立了食品中碱性黄、碱性嫩黄、碱性橙II、酸性橙II、罗丹明B、对位红、苏丹类1-4号11种有机染料的高效液相色谱分析方法。在0.2-10μg/mL的浓度范围内线性相关系数良好,相关系数范围为0.9996-0.9999,检测限0.05μg/mL。该方法涉及的化学试剂少,操作简便,结果准确。对阳性样品进一步采用液质联用技术确证。     

An animal component free medium that promotes the growth of various animal cell lines for the production of viral vaccines

IPT-AFM is a proprietary animal component free medium that was developed for rabies virus (strain LP 2061) production in Vero cells. In the present work, we demonstrated the versatility of this medium and its ability to sustain the growth of other cell lines and different virus strains. Here, three models were presented: Vero cells/rabies virus (strain LP 2061), MRC-5 cells/measles virus (strain AIK-C) and BHK-21 cells/rabies virus (strain PV-BHK21). The cell lines were first adapted to grow in IPT-AFM, by progressive reduction of the amount of serum in the culture medium. After their adaptation, BHK-21 cells grew in suspension by forming clumps, whereas MRC-5 cells remained adherent. Then, kinetics of cell growth were studied in agitated cultures for both cell lines. In addition, kinetics of virus replication were investigated.