Depression, Night Terrors, and Insomnia Associated With Long-Term Intrathecal Clonidine Therapy

Abstract:   Intraspinal clonidine is an effective adjunct to intrathecal/epidural opioid administration. We report a case of neuropathic pain treated with intraspinal analgesics in which depression, insomnia, and night terrors developed in association with intraspinal clonidine.     

人肺癌细胞转录因子--C/EBP和HMG样蛋白的EMSA检测

目的：检测人肺癌细胞中是否存在与Na^ /H^ 交换蛋白-1（NHE-1）基因转录相关的重要转录因子--C/EBP和HMG样蛋白。方法：采用电泳迁移率改变分析法（EMSA）检测人肺癌细胞株核蛋白中C/EBP和HMG样蛋白的表达及其水平。结果：EMSA检测到A549细胞和转染NHE-1反义载体的A549细胞均有转录因子C/EBP和HMG样蛋白的表达，且后者表达水平高于前者；采用50倍未标记的片段能完全抑制相应转录因子与^32P标记的相同片段的结合。结论：A549细胞核蛋白中存在能与C/EBP结合序列和Poly(dA：dT)区结合的转录因子，即C/EBP和HMG样蛋白，后者的表达水平高于前者。特异性竞争抑制试验表明外源性特异DNA片段能抑制相同片段与转录因子的结合。     

The arthroscopic monopolar radiofrequency treatment of chronic anterior cruciate ligament instability

The Holmium:YAG (Ho:YAG) laser is the arthroscopic laser of choice. The arthroscopic surgeon can ablate, coagulate, or shrink periarticular soft tissues by manipulating Ho:YAG laser power settings. The ability to ablate soft tissue in a hemostatic fashion greatly facilitates the treatment of labral tear, synovitis, subacromial arch decompression, and distal clavicle arthritis. The nonablative application of Ho:YAG laser energy has been used successfully to treat glenohumeral instability. The laser-assisted capsular shift (LACS) procedure, in conjunction with standard labral repair techniques, successfully treats unidirectional and multidirectional shoulder instability Neuromuscular rehabilitation is accelerated. Excellent clinical results may be achieved with appropriate surgical technique and postoperative rehabilitation.     

Right pneumonectomy syndrome: Report of two cases

We report herein the cases of two infants who developed right pneumonectomy syndrome, both of whom were born with gross C-type esophageal atresia (EA/TEF), and a hypoplastic right lung arising from the lower esophagus, being a bronchopulmonary foregut malformation (BPFM). Appropriate and well-timed treatments for a variety of sequelae primarily caused by the mediastinal shift must be considered after right pneumonectomy in early childhood.     

Large rod-like photopic signals in a possible new form of congenital night blindness

A 10-year-old Persian girl has symptoms of congenital stationary night blindness and some drusen-like lesions in the region of the vascular arcades. Her electroretinogram shows no rod response to a weak stimulus, but a large (475 V) slow scotopic response to a strong stimulus that is unchanged by photopic conditions (15 F1 background illumination). However, the response to flicker had the typical (smaller) amplitude of a cone signal. This may represent a new form of night blindness in which rod sensitivity is reduced so that there is no vision under dim conditions but rod function still persists under photopic conditions.     

Effects of long working hours and the night shift on severe sleepiness among workers with 12‐hour shift systems for 5 to 7 consecutive days in the automobile factories of Korea

We investigated the effects of 12‐hour shift work for five to seven consecutive days and overtime on the prevalence of severe sleepiness in the automobile industry in Korea. [Correction added after online publication 28 Nov: Opening sentence of the summary has been rephrased for better clarity.] A total of 288 randomly selected male workers from two automobile factories were selected and investigated using questionnaires and sleep‐wake diaries in South Korea. The prevalence of severe sleepiness at work [i.e. Karolinska Sleepiness Scale (KSS) score of 7 or higher] was modeled using marginal logistic regression and included theoretical risk factors related to working hours and potential confounding factors related to socio‐economic status, work demands, and health behaviors. Factors related to working hours increased the risk for severe sleepiness at the end of the shift in the following order: the night shift [odds ratio (OR): 4.7; 95% confidence interval (CI): 3.6–6.0)], daily overtime (OR: 2.2; 95% CI: 1.7–2.9), weekly overtime (OR: 1.6; 95% CI: 1.0–2.6), and night overtime (OR: 1.6; 95% CI: 0.8–3.0). Long working hours and shift work had a significant interactive effect for severe sleepiness at work. Night shift workers who worked for 12 h or more a day were exposed to a risk of severe sleepiness that was 7.5 times greater than day shift workers who worked less than 11 h. Night shifts and long working hours were the main risk factors for severe sleepiness among automobile factory workers in Korea. Night shifts and long working hours have a high degree of interactive effects resulting in severe sleepiness at work, which highlight the need for immediate measures to address these characteristics among South Korean labor force patterns.     

基于形态学的均值平移算法的神经元干细胞图像的自动分割

在神经元干细胞的图像分析中,准确快速的图像分割是干细胞分化增值自动追踪系统的基础。为了准确地分割低对比度的灰度神经元干细胞图像,本研究提出一种基于形态学运算和均值平移算法的神经元干细胞分割方法,称其为形态学的均值平移算法。此算法可以快速地获得任意形状细胞的图像,并且能检测到图像中多连接边缘不封闭的细胞。将此方法应用于神经元干细胞序列图像分割中并且将其与门限分割、水线分割和活动轮廓进行对比。实验结果证明,与其它的方法相比,此方法获得的细胞分割形状更接近于真实细胞的形状,并且能获得或接近于原始图像中准确的独立细胞数目。此方法可以获得正确的分割结果,为进一步图像处理奠定了良好的基础。     

心室晚电位检测中消除振铃方法的研究

ＦＧＩＲ作者着重研究了心室晚电位（简称ＶＬＰ）检测中，进行带通滤波时，振铃现象对晚电位检测的影响及消除。我们采用双向滤波、最小相移滤波及ＦＩＲ滤波等方法探讨了振铃对ＶＬＰ的影响，比较了各种方法对振铃的抑制作用。为了考察各种方法对ＶＬＰ检出能力，作者采用了在相干平均后的心电波形上桑加与真实情况一致的仿真ＶＬＰ形的方法，比较了上述方法对ＶＬＰ的检出作用，并在证实了传统双向滤波对振铃作用的基础上，从理论     

迁移率改变分析

本文介绍另一种检测特异顺序的DNA结合蛋白的理想方法——迁移率改变法。该方法是在特定的缓冲液中,蛋白质与带有同位素标记的DNA探针进行结合反应,然后在低离子强度的电泳缓冲液中,电泳分离DNA与蛋白质结合的复合物与未结合的游离DNA探针,进而鉴定特异顺序的DNA结合蛋白。