牙本质内Ⅰ型胶原纤维形态的免疫标记初探

目的初步探讨进行免疫标记的牙本质脱矿后暴露的Ⅰ型胶原纤维。方法利用免疫组化技术和场发射扫描电镜(FEISEM)相结合,对暴露胶原纤维的形态以及结构完整性进行评价。结果通过FEISEM观察,牙本质脱矿后暴露的Ⅰ型胶原纤维,可以结合抗Ⅰ型胶原单克隆抗体以及胶体金标记的相应二抗,胶体金标记颗粒分布均匀,可与暴露的胶原纤维上的抗原表位相结合,实现免疫定位。结论免疫组化技术和FEISEM相结合,可以直观观察胶原纤维立体网络的超微结构,是一种准确的、可复制的、可行的方法 。     

The vial kit formulation for preparation of no‐carrier‐added 131I‐MIBG

We have developed a new set of lyophilized kits, composed of 3 different kits, for the instant preparation of no‐carrier‐added ¹³¹I‐MIBG in the clinic. We here discussed the formulation of the kits, optimization of radiolabelling, quality control of radiolabeled ¹³¹I‐MIBG, and studies of animal biodistribution. The no‐carrier‐added (nca) ¹³¹I‐MIBG injection could be prepared within 30 minutes in the clinic with the help of the lyophilized kits. The radiochemical purity and specific activity (SA) could achieve above 98% and 6700 MBq/mg, respectively.     

Synthesis,bioconjugation and stability studies of [18F]ethenesulfonyl fluoride

Fluorine‐18 labelled prosthetic groups (PGs) are often necessary for radiolabelling sensitive biological molecules such as peptides and proteins. Several shortcomings, however, often diminish the final yield of radiotracer. In an attempt to provide higher yielding and operationally efficient tools for radiolabelling biological molecules, we describe herein the first radiochemical synthesis of [¹⁸F]ethenesulfonyl fluoride ([¹⁸F]ESF) and its Michael conjugation with amino acids and proteins. The synthesis of [¹⁸F]ESF was optimised using a microfluidic reactor under both carrier‐added (c.a.) and no‐carrier‐added (n.c.a.) conditions, affording, in a straightforward procedure, 30‐50% radiochemical yield (RCY) for c.a. [¹⁸F]ESF and 60‐70% RCY for n.c.a. [¹⁸F]ESF. The conjugation reactions were performed at room temperature using 10 mg/mL precursor in aqueous/organic solvent mixtures for 15 min. The radiochemical stability of the final conjugates was evaluated in injectable formulation and rat serum, and resulted strongly substrate dependent and generally poor in rat serum. Therefore, in this work we have optimised a straightforward synthesis of [¹⁸F]ESF and its Michael conjugation with model compounds, without requiring chromatographic purification. However, given the general low stability of the final products, further studies will be required for improving conjugate stability, before assessing the use of this PG for PET imaging.     

Assessing the Average Sodium Content of Prepacked Foods with Nutrition Declarations: The Importance of Sales Data

Processed foods are recognized as a major contributor to high dietary sodium intake, associated with increased risk of cardiovascular disease. Different public health actions are being introduced to reduce sodium content in processed foods and sodium intake in general. A gradual reduction of sodium content in processed foods was proposed in Slovenia, but monitoring sodium content in the food supply is essential to evaluate the progress. Our primary objective was to test a new approach for assessing the sales-weighted average sodium content of prepacked foods on the market. We show that a combination of 12-month food sales data provided by food retailers covering the majority of the national market and a comprehensive food composition database compiled using food labelling data represent a robust and cost-effective approach to assessing the sales-weighted average sodium content of prepacked foods. Food categories with the highest sodium content were processed meats (particularly dry cured meat), ready meals (especially frozen pizza) and cheese. The reported results show that in most investigated food categories, market leaders in the Slovenian market have lower sodium contents than the category average. The proposed method represents an excellent tool for monitoring sodium content in the food supply.     

Synthesis of [18O2]‐curcumin

Curcumin, a pigment isolated from rhizomes of Curcuma longa, is a potent cancer chemopreventive and chemotherapeutic agent and is now evaluated in phase III human clinical trials. This report describes an efficient synthesis of [¹⁸O₂]‐curcumin. [¹⁸O₂]‐Curcumin was prepared in three steps from 1‐iodo‐2‐methoxy‐4‐methylbenzene in an overall yield of 53%.     

Amino Surface Modification and Fluorescent Labelling of Porous Hollow Organosilica Particles: Optimization and Characterization

Surface modification of silica nanoparticles with organic functional groups while maintaining colloidal stability remains a synthetic challenge. This work aimed to prepare highly dispersed porous hollow organosilica particles (pHOPs) with amino surface modification. The amino-surface modification of pHOPs was carried out with 3-aminopropyl(diethoxy)methylsilane (APDEMS) under various reaction parameters, and the optimal pHOP-NH₂ sample was selected and labelled with fluorescein isothiocyanate (FITC) to achieve fluorescent pHOPs (F-HOPs). The prepared pHOPs were thoroughly characterized by transmission electron microscopy, dynamic light scattering, FT-IR, UV-Vis and fluorescence spectroscopies, and microfluidic resistive pulse sensing. The optimal amino surface modification of pHOPs with APDEMS was at pH 10.2, at 60 °C temperature with 10 min reaction time. The positive Zeta potential of pHOP-NH₂ in an acidic environment and the appearance of vibrations characteristic to the surface amino groups on the FT-IR spectra prove the successful surface modification. A red-shift in the absorbance spectrum and the appearance of bands characteristic to secondary amines in the FTIR spectrum of F-HOP confirmed the covalent attachment of FITC to pHOP-NH₂. This study provides a step-by-step synthetic optimization and characterization of fluorescently labelled organosilica particles to enhance their optical properties and extend their applications.     

大鼠终纹床核向结合臂旁核的投射 Ⅲ.神经降压肽、促肾上腺皮质激素释放因子肽能通路研究

用荧光金逆行束路追踪结合免疫荧光双标记技术及HRP逆行束路追踪结合免疫细胞化学双标记技术证明,在大鼠终纹床核投射至结合臂旁核的神经元中,位于前外侧区卵圆核及其腹侧邻近区中的部分为神经降压肽及促肾上腺皮质激素释放因子免疫反应阳性,双标记细胞分别约占这个部位逆行标记细胞总数的40％及20％;在前腹侧区的梭形核及大细胞核中,部分为促肾上腺皮质激素释放因子免疫反应阳性。     

125I标记Toll样受体4-抗体与人单核细胞系THP1的体外结合研究

目的：125I标记Toll样受体4抗体（TLR4）研究其与人单核细胞系THP1细胞体外结合的特性。方法：用125I标记TLR4,以快速硅胶簿层层析纸（ITLC-SG）分析方法鉴定125I标记抗体的放射化学纯度,再用γ计数器测定125I标记抗体与THP1细胞的TLR4结合量,其中特异结合（SB）=总结合管（TB）-非特异管（NSB）。结果：125I标记TLR4-Ab的标记率为80.27%（n=7）。用快速硅胶簿层层析纸（ITLC-SG）分析方法鉴定其放射化学纯度都能〉95%,测定125I标记抗体的比活度为3.145TBq.mg-1。结论：125I标记的TLR4-Ab具有良好的标记率和放射化学纯度,且125I标记TLR4-Ab与THP1细胞在体外具有很高结合亲和力。     

Circadian rhythms in the number of gastrin cells,DNA synthesizing cells and labelling index of gastrin cells in the antral mucosa of the rat

Circadian variations of the gastrin cell (G-cell) number, the DNA synthesizing cell (S phase cell) number and labelling index of G-cell in antral mucosa were studied using the simultaneous double immunoenzymatic labelling method of gastrin and bromodeoxyuridine (BrdU) both in fed and 24 h fasted rats. No significant change was observed in the G-cell number. The S phase cell number and labelling index of G-cell showed significant circadian rhythms. Labelling index of G-cell markedly decreased in 24 h fasted rats in comparison to that in fed rats. These results suggest that DNA synthesis in G-cells has a circadian rhythm and that the activity is influenced by food ingestion.