体外构建色素化皮肤类似物模型

目的 探寻构建色素化皮肤类似物的方法.方法 以包皮组织作为细胞来源,采用消化法获得角质形成细胞、黑素细胞;采用组织块法获得成纤维细胞,以鼠尾胶原为支架,构建色素化皮肤类似物.HE染色、Fontana Masson染色检测构建的色素化皮肤类似物的大体结构及其中黑色素细胞的分布和状态,以透射电镜观察类似物的超微结构.结果 构建的色素化皮肤类似物结构完整,细胞状态良好,与皮肤结构相似.结论 成功构建了色素化皮肤类似物模型.     

Resveratrol Inhibits Secretion of Interleukin 8 by Regulation of Autophagic Flux in Ultraviolet B-stimulated Keratinocytes

Objective:The plant polyphenol resveratrol(3,4',5-trihydroxystilbene)(RSV)has been proposed for use because of its protective effect on ultraviolet(UV)-induced ...     

Particulate Matters Induce Apoptosis in Human Hair Follicular Keratinocytes

BackgroundParticulate matters (PM) comprise a heterogeneous mixture of particles suspended in air. A recent study found that urban PMs may penetrate into hair follicles via transfollicular and transdermal routes in dorsal skin.ObjectiveTo investigate the effects of PM on ex vivo cultured human scalp hair follicles and hair follicular keratinocytes in vitro.MethodsTUNEL staining was employed to check cells undergoing apoptosis in cultured hair follicles after PM treatment. MTT assay was employed to check cell viability after PM treatment. Quantitative real-time PCR analysis was employed to quantitate the expression of inflammatory genes, matrix metalloproteinases (MMPs), and Duox1. Inflammatory cytokine levels were measured by ELISA after PM treatment. The level of reactive oxygen species (ROS) production was measured using a chemical fluorescent probe by a fluorescence plate reader.ResultsAbundant TUNEL-positive cells were observed in the keratinocyte region of hair including the epidermis, sebaceous gland, outer root sheath (ORS), inner root sheath (IRS), and bulb region. The viability of follicular cells, including the ORS, was found to be decreased upon PM exposure. mRNA expression and protein levels of inflammatory response genes and MMPs were upregulated in a dose-dependent manner by PM treatment. ROS levels were also increased by PM.ConclusionThese data strongly suggest that penetrated PMs from air pollution may cause apoptotic cell death to follicular keratinocytes by increased production of ROS and inflammatory cytokines, which could impair hair growth.