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71.
母婴ABO血型不合与新生儿溶血病的关系 总被引:4,自引:0,他引:4
目的 探讨O型孕妇血清IgG抗体效价与新生儿溶血病的关系。 方法 对丈夫血型为A型 ,B型或AB型的 75 4例O型孕妇进行血清IgG抗A(B)抗体水平检测 ,同时对IgG抗A(B)≥ 6 4的孕妇所生的新生儿做HDN免疫血清学检查。 结果 75 4例O型孕妇中产前IgG抗A(B)≥ 6 4的有 2 87例 ,其所生新生儿发生新生儿溶血病的例数为 31例。结论 新生儿溶血病发病率与孕妇血清IgG抗A(B)效价的高低成正相关。 相似文献
72.
In enamel fluorosis model rats treated with sodium fluoride, secretory ameloblasts of incisor tooth germs exhibited disruption
of intracellular trafficking. We examined whether heterotrimeric G proteins participated in the disruption of vesicular trafficking
of the secretory ameloblast exposed to fluoride, using immunoblotting and pertussis toxin (IAP)-induced adenosyl diphosphate
(ADP)-ribosylation for membrane fractions of the cell. Immunoblotting of crude membranes, post supernatants of the ameloblast,
with anti-Gi3/o and anti-Gs antibodies showed that Gi3 or Go proteins existed in the secretory ameloblast, but Gs protein did not. Immunoblotting of the subcellular membrane fractions indicated that the Gi3 or Go proteins were located in the Golgi membrane, but were not in the rough endoplasmic reticulum (rER) membrane. Autoradiograph
of IAP-induced ADP-ribosylation, however, showed the existence of IAP-sensitive G proteins both in rER and Golgi membranes.
Fluoride treatment decreased the G proteins bound to both membranes. These findings indicate that different G proteins, both
of which are IAP-sensitive, are present in the rER and Golgi apparatus, and suggest that these G proteins participate in the
disturbance of intracellular transport of the secretory ameloblast exposed to fluoride.
Received: 24 June 1998 / Accepted: 8 September 1998 相似文献
73.
Carmelo Romano Michael A. Sesma Colin T. McDonald Karen O'malley Anthony N. van den Pol John W. Olney 《The Journal of comparative neurology》1995,355(3):455-469
The receptor mGluR5 is a metabotropic glutamate receptor with messenger RNA abundantly present throughout cortex, hippocampus, and caudate/putamen that is also coupled to phosphatidyl inositide hydrolysis and calcium mobilization. In this study, the distribution of mGluR5 was examined in rat brain by immunocytochemistry. The antibody utilized is highly specific and does not cross react with the most closely related other metabotropic glutamate receptor, as determined by Western blot analysis of nonneuronal cells transfected with metabotropic receptor coding sequences. The receptor mGluR5 is widely expressed with the highest density in olfactory bulb, caudate/putamen, lateral septum, cortex, and hippocampus, as confirmed with both immunocytochemistry and Western blot analysis. Electron microscopic studies in hippocampus and cortex indicate that the labeling is mostly on membranes of dendritic spines and shafts. Light and electron microscopic evidence indicates that some mGluR5 immunoreactivity is located in presynaptic axon terminals, suggesting that mGluR5 may function as a presynaptic receptor. 相似文献
74.
75.
高性能战斗机飞行员离心机高G训练方案 总被引:13,自引:5,他引:8
目的制定我国高性能战斗机飞行员离心机高G训练方案。方法拟定离心机高G训练方案,对47名飞行员进行训练,训练后检验训练效果,提出训练方案。结果训练后受试者采用综合抗荷措施时的G耐力比基础G耐力高4.3G~4.7G,比训练前提高了1.9G~3.3G(P<0.01)。结论按本研究提出的高G训练方案进行训练,可显著提高飞行员的G耐力,该训练方案有效可行。 相似文献
76.
κ及λ轻链测定在多发性骨髓瘤诊断中的应用 总被引:2,自引:0,他引:2
目的:寻求准确,可靠的免疫球蛋白及κ和λ轻链测定方法,提高多发性骨髓瘤分型和鉴别诊断水平。 相似文献
77.
78.
过氧化氢对白色念珠菌DNA破坏的研究 总被引:1,自引:0,他引:1
本文用热变性温度法研究一定浓度 H_2O_2对 C.albicans DNA 破坏作用。结果表明,经6%和10%H_2O_2处理后,C.albicans DNA G c Mol%(分别为28.1±0.82和14.4±1.51)显著低于对照 DNA G C Mol%(85.9±0.62),并推断 H_2O_2可能作用于 C.albicans DNA 碱基以及其间的氢键上;DNA 电泳图谱发现,经3%和6%H_2O_2处理的 C.albicans DNA 电泳带明显不同于对照,提示 H_2O_2更易造成 C.albicans DNA 核苷酸链的降解或断裂.H_2O_2对 C.albicans DNA 的破坏,增强了其杀菌作用。 相似文献
79.
Clinical efficacy of intravenous immunoglobulin in patients with severe inflammatory chest disease and IgG3 subclass deficiency
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E Bernatowska-Matuszkiewicz M Pac H Skopcynska M Pum M M Eibl 《Clinical and experimental immunology》1991,85(2):193-197
To investigate the efficacy of i.v. IgG treatment in pediatric patients with inflammatory lung disease, a prospective, controlled clinical trial was carried out over a 2-year study period. Patients were enrolled on the basis of severe clinical symptomatology. After 1 year of conventional treatment, the patients received 400 mg/kg per month of an i.v. IgG product containing only trace amounts of IgG3 in addition to their regular treatment throughout the second year. Significant clinical improvement, as documented by duration of hospital stay (first year 27.8 days, second year 4.9 days), use of antibiotics (132.8 versus 30.9 days) and use of steroids (21.4 versus 0.7 days) could be observed. Data obtained on a subgroup of patients with IgG3 deficiency were analysed separately. These results indicate that patients with severe chest disease who have IgG3 deficiency will also benefit from i.v. IgG treatment. The mode of action cannot be attributed to replacement of the respective isotypes, but is probably due to the effect of i.v. IgG in preventing repeated viral infections. 相似文献
80.
It was recently shown that streptokinase may induce clot formation in vivo by immunoglobulin G mediated platelet stimulation. We evaluated the in vitro effect of streptokinase on platelet function in 103 subjects, of whom 52 were < or = 30 years and 51 were > or = 50 years old. Although streptokinase inhibited platelet aggregation in the majority of cases, in nine the threshold concentration of ADP required to induce irreversible aggregation decreased with streptokinase (1 million Units. l-1) by 30% or more. This observation was confirmed in five of the nine by repeated measurements indicating reproducible streptokinase-induced platelet stimulation. Among the five, two were < or = 30, and three were > or = 50 years old. In none of the five subjects did the radio allergo sorbent test detect type E immunoglobulins directed against streptokinase in the serum. In contrast, in four of the five subjects, streptokinase-induced platelet hyperaggregability was suppressed by addition of goat antibodies against human immunoglobulin G, or F(ab')2-fragments of such antibodies. Acetylsalicylic acid did not prevent streptokinase-induced platelet stimulation, but in three of five cases, led to an increase in the control threshold concentration for ADP, so that after the decrease induced by streptokinase the threshold concentration for ADP was in the same range as before acetylsalicylic acid and streptokinase administration. Thus, streptokinase led to an inhibition of platelet aggregation in the majority of subjects evaluated. In a minority of five out of 103, however, streptokinase reproducibly caused platelet stimulation, presumably mediated by immunoglobulin G.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献