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161.
金桂红 《安徽卫生职业技术学院学报》2005,4(6):35-36
目的:探讨小儿腮腺炎并发中枢神经系统感染的临床特点.方法:对52例小儿腮腺炎并发中枢神经系统感染的临床资料进行回顾性总结分析.结果:3~7月份为小儿腮腺炎并发中枢神经系统感染的发病高峰季节(92.31%).发病年龄以6~12岁为主(82.69%),多发生于腮腺肿痛3天以上伴有持续发热者(78.85%),少数患者同时有其它脏器的损害,一般治疗疗效较好.结论:腮腺炎极易并发中枢神经系统感染,年长儿发生率高,应推广接种流腮活疫苗降低发病率. 相似文献
162.
Sundaram Hariharan Eric P. Cohen Brahm Vasudev Rimas Orentas Raphael P. Viscidi Justin Kakela Brian DuChateau 《American journal of transplantation》2005,5(11):2719-2724
We evaluated twenty renal transplant subjects at various stages of BKV nephritis (BKVN) for BKV-specific IgG and IgM antibodies using ELISA technique and BKV-DNA using PCR. They were divided as early onset (n = 7), stabilizing (n = 3), resolved (n = 8) and late onset (n = 2) BKVN. BKV-specific antibodies and BKV-DNA were simultaneously determined. The mean BKV-specific IgG level in early onset and stabilizing BKVN were 64 and 39 EIA units, and were significantly lower than 138 EIA units seen in resolved BKVN, P = 0.007, P = 0.008. The mean BKV-specific IgM levels in stabilizing BKVN was higher than resolved BKVN (130 vs 51 EIA units), P = 0.006. Mean plasma BKV loads for each group were 955,925, 5642 and 42 copies/mL of plasma, respectively. Prospective study in six BKVN cases revealed mean IgG, IgM levels and BKV-DNA at the time of diagnosis of BKVN as 39, 110 EIA units and 586,758 copies/mL of plasma, respectively. After a mean period of 5.2 months, IgG level increased to 120 EIA units (p = 0.0058) and had no detectable viral copies in circulation. Recovery from BKVN and elimination of BKV is associated with the development of BKV-specific IgG antibodies and this provides insight into the role of humoral immunity to BKV in the pathogenesis of BKVN. 相似文献
163.
目的:从血清生化,病毒学,肝纤维化指标以及肝脏组织病理学改变的角度分析拉米夫定(LMD)治疗慢性乙型肝炎的疗效.方法:慢性乙型肝炎患者21例,给予口服LMD100 mg/d,连用1 a,动态观察服药0,24和48 wk肝功能、乙肝五项、HBV-DNA定量、血清肝纤维化指标透明质酸(HA)、层黏蛋白(LN)、Ⅲ型前胶原(PⅢP)和Ⅳ型胶原(ⅣC)的变化,通过肝组织穿刺活检,观察用药前后肝脏组织病理学的改变.结果:LMD治疗48 wk,可显著抑制HBV-DNA(copy/L)复制(6.13×109±4.03×105 vs 9.01×105±4.89×103,P<0.01),使大多数患者肝功能(nkat/L,ALT:1697±907 vs550±503;AST:1787±717 vs 498±430)恢复正常(P<0.01),显著降低血清肝纤维化指标水平(P<0.01).减轻肝细胞坏死,汇管区炎细胞浸润及纤维化.结论:LMD是治疗慢性乙型肝炎的一种较为切实有效的措施. 相似文献
164.
Tae-Jin Song M.D. Ph.D. David P. Eisenberg M.D. Prasad S. Adusumilli M.D. Michael Hezel B.S. Yuman Fong M.D. 《Journal of gastrointestinal surgery》2006,10(4):532-542
The rising incidence of hepatocellular carcinoma (HCC) in western countries, along with the poor prognosis offered by present-day
treatment modalities, makes novel therapies for this disease necessary. Oncolytic herpes simplex viruses (HSV) are replication-competent
viruses that are highly effective in the treatment of a wide variety of experimental models of human malignancies. This study
seeks to investigate the effectiveness of oncolytic herpes viruses in the treatment of primary HCC cell lines. Sixteen commercially
available human HCC cell lines were studied. G207 is an attenuated, replication-competent, oncolytic HSV engineered to selectively
replicate within cancer cells. Cell lines were tested for viral sensitivity to G207 and their ability to support viral replication
using standard cytotoxicity and viral replication assays. Eleven of 16 cell lines were moderately to highly sensitive to G207
viral oncolysis. HCC cell lines additionally demonstrated the ability to support viral replication in vitro with as high as
800-fold amplification of the administered viral dose observed. G207 is cytotoxic to, and efficiently replicates within, HCC
cell lines in vitro. From these data, we suggest that oncolytic HSV therapy may have a role in the treatment of HCC, and in
vivo studies are warranted.
Presented in part at the 2005 American Hepato-Pancreato-Biliary Association Congress, Hollywood, Florida, April 14–17, 2005.
Supported by grants R01CA75461 and R01CA72632 from the National Institutes of Health, and by grant MBC-99366 from the American
Cancer Society (Yuman Fong). 相似文献
165.
人脑外伤后皮层核因子-κB的表达 总被引:5,自引:0,他引:5
目的 探讨核因子-κB(nuclear factor—κB,NF—κB)在人创伤性脑损伤(traumatic brain injury,TBI)后挫伤皮层中的表达情况,包括表达位置、表达强度和表达时相。方法 挫伤区皮层的标本来自24例TBI患者,取样时间为伤后5h-5d,利用凝胶电泳迁移分析法(electrophoretic mobility shift assay,EMSA)和免疫组化技术测定NF—κB的活性和表达强度。结果 在人TBI后的挫伤区皮层中,NF—κB表达明显上调,表达高峰为伤后48—72h,NF—κBP65主要在血管内皮细胞和神经胶质细胞中表达,NF—κBP50主要在神经胶质细胞和少量神经元中表达,NF—κBP65的表达强度高于NF—κBP50。结论 NF—κB在人TBI后的挫伤区皮层中表达上调,提示其可能在TBI后的病理生理过程中起着重要作用。 相似文献
166.
167.
Robert Motzer Howard Scher Dean Bajorin Cora Sternberg George J. Bosl 《Investigational new drugs》1990,8(4):391-392
Summary Twenty-three patients with advanced renal cell cancer were treated with Didemnin B. One partial response was achieved (5%) in 21 evaluable patients. An allergic reaction was noted in four patients including one patient with anaphylaxis. Didemnin B is not recommended in the treatment of renal cell carcinoma. 相似文献
168.
D. McCormick 《Neuropathology and applied neurobiology》1993,19(2):146-151
There is evidence from investigations of non-CNS neoplasms that secreted proteolytic enzymes may facilitate tumour invasion by partially degrading extracellular matrix (ECM). Among the enzymes which may be involved are members of the cysteine proteinase superfamily and especially cathepsin B (CB). In the present investigation we have studied CB in human gliomas in vitro , concentrating particularly on CB secretion, as extracellular enzyme is of prime importance in this context. We have found that CB is secreted by gliomas in vitro as a latent zymogen, requiring activation. This has been confirmed by gel chromatography which indicated that CB is secreted as a 42 kDa proenzyme which may be proteolytically processed to an enzymatically active 29 kDa molecule. The inactive, high molecular weight, latent CB is stable at extracellular pH in contrast to the activated low molecular weight form which rapidly loses activity at this pH. We have also measured secretion of cysteine proteinase inhibitors (CPI), as their presence would have a direct influence on the effective activity of CB, and found that all of the gliomas secreted significant amounts of a CPI as assessed by papain inhibition. Our experiments suggest that a number of factors are involved in the regulation of extracellular glioma-derived CB activity. These include: rate of secretion of pro-CB, rate of CB activation, destabilization of CB at neutral pH and the presence of cysteine proteinase inhibitors. 相似文献
169.
Zakiya Al-Lamki Eileen Thomas Nagwa El-Banna Norman Jaffe 《Pediatric blood & cancer》1995,24(2):137-140
We report an unusual case of anaphylaxis and hepatitic dysfunction in a child with the administration of the twenty-third course of high-dose methotrexate. The latter had been used as an adjuvant to prevent pulmonary metastases and the prior 22 courses had been well tolerated. An attempt to reinstate methotrexate after the twenty-third course was again followed by a similar reaction. © 1995 Wiley-Liss, Inc. 相似文献
170.
I. Tsunoda Yuzo Iwasaki Hiroshi Terunuma Kazuya Sako Yoshiro Ohara 《Acta neuropathologica》1996,91(6):595-602
Theiler’s murine encephalomyelitis viruses (TMEV) are divided into two subgroups on the basis of their different biological
activities. The GDVII strain produces acute polioencephalomyelitis in mice, whereas the DA strain produces demyelination with
virus persistence in the spinal cord. A comparative study of GDVII and DA strains suggested that low host immune responses
are responsible for the development of acute GDVII infection and that the persistence of infected macrophages plays a crucial
role in the development of chronic white matter lesions in DA infection. All 78 mice infected with GDVII died or became moribund
by day 13, while none of 54 mice infected with DA died. In the acute stage, the distribution of viral antigens in the central
nervous system (CNS) tissue was similar in both GDVII and DA infections, although the virus titer was higher in GDVII infection.
In DA infection, a substantial number of T cells were recruited to the CNS on day 6 when they were virtually absent in GDVII
infection. The titer of neutralizing antibody was already high on day 6 in DA infection but was negligible in GDVII infection.
Development of chronic paralytic disease from day 35 of the DA infection was accompanied by focal accumulation of viral antigen-positive
macrophages in the spinal white matter. In addition, white matter lesions comparable to those in chronic DA infection were
induced in the spinal cord within 7 days after intracerebral injection of DA-infected murine macrophages.
Received: 26 June 1995 / Revised, accepted: 27 December 1995 相似文献