Monoammonium glycyrrhizinate inhibited the inflammation of LPS-induced acute lung injury in mice

Monoammonium glycyrrhizinate (MAG) was the aglycone of glycyrrhizin derived from licorice. In this study, the anti-inflammatory effects of MAG on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and the possible mechanisms involved in this protection were investigated. Pretreatment with MAG prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight/dry weight ratio, in total leukocyte number and neutrophil percent in the BALF, and in myeloperoxidase (MPO) activity of lung in dose-dependent manners. At the same time, pretreatment with MAG also significantly improved the super oxide dismutase (SOD) activity and induced the malondialdehyde (MDA) content in the bronchoalveolar lavage fluid (BALF). Importantly, pretreatment with MAG prevented an increase in cyclic adenosine monophosphate-phosphodiesterase (cAMP-PDE) activity of lung in a dose-dependent manner. In addition, it can up-regulate the interleukin-10 (IL-10) level and down-regulate the tumor neurosis factor-α (TNF-α) level in the lung tissue of ALI mice. These results showed that anti-inflammatory effects of MAG against the LPS-induced ALI may be due to its ability of primary inhibition of cAMP-PDE activity, oxidative stress and its regulation of cytokine effects. Thus the results support that use of MAG is beneficial in the treatment of ALI and ARDS.     

甘草酸二铵对脊髓缺血再灌注损伤后IL-4/IL-8/TNF-α表达的影响

目的初步探讨甘草酸二铵（Diammonium Glycyrrhizinate,DG）对脊髓缺血再灌注损伤后脊髓组织中炎性细胞因子IL-4、IL-8、TNF-α表达的影响。方法雄性SD大鼠90只,随机分为空白对照组、阴性对照组、DG干预组三组,通过外科手术法造成大鼠腰段脊髓的缺血再灌注损伤,组织匀浆后采用ELISA检测IL-4、IL-8、TNF-α的表达。结果与阴性对照组比较：空白对照组IL-4在24、72、168h有统计学意义（P〈0.05）,DG干预组IL-4在3、24h时间段有统计学意义（P〈0.05）;阴性对照组IL-8各时间段均有统计学意义（P〈0.05）;DG干预组IL-824、72h时间段有统计学意义（P〈0.05）;空白对照组TNF-α各时间段均有统计学意义（P〈0.05）;DG干预组TNF-α全部时间段均有统计学意义（P〈0.05）。结论脊髓缺血再灌注损伤后IL-4、IL-8、TNF-α的表达明显发生变化,DG可能通过影响IL-4、IL-8、TNF-α的表达发挥保护作用。     

甘利欣治疗支气管肺发育不良的疗效评价

目的:评价甘利欣在支气管肺发育不良(BPD)治疗中的临床疗效。方法:2004年6月～2007年12月入住本院新生儿科的BPD患儿52例,随机分为治疗组和对照组,治疗组(27例)在对照组(25例)常规治疗基础上加用甘利欣治疗,观察两组患儿经皮氧饱和度(TcSaO2)、血气分析变化情况以及氧疗时间、肝肾功能、电解质紊乱、高血压、高血糖和感染等情况,以及纠正胎龄37周时神经行为评分(NBNA)。结果:治疗组在应用甘利欣治疗7天后各项监测指标变化与对照组差异无统计学意义(P>0.05),应用14天后,PO2明显升高、PCO2明显降低,同时氧疗时间明显缩短,差异具有统计学意义(P<0.05),肝肾功能异常、电解质紊乱、高血压、高血糖和感染等的发生率及NBNA评分结果两组间差异无统计学意义。结论:甘利欣能改善BPD患儿的肺脏功能,减少对氧的依赖,利于临床症状的改善,且无明显毒副作用,值得应用。     

Deformable liposomes for dermal administration of methotrexate

Deformable liposomes were prepared to investigate the effectiveness of dermal administration of methotrexate (MTX). The phospholipids used to prepare the liposomes were soybean lecithin (PC) or hydrogenated lecithin (HPC) and dipotassium glycyrrhizinate (KG) as surfactant. The lipid/KG ratio (w/w) was 2:1 and 4:1. Liposomes size, entrapment efficiency and MTX release through dialysis membrane were determined and the interaction between MTX and liposomes was investigated using differential scanning calorimetry. The MTX amount permeated through pig skin were three- to four-fold higher using liposomes containing KG compared to those from water solution or normal liposomes. No significant differences were observed between PC-KG liposomes and HPC-KG liposomes. At the end of the skin permeation assay using deformable liposomes, up to 50% of the administered dose was found in the skin. This capability depends on the self-regulating carrier deformability. These results suggest that liposomes containing KG may be of value for the topical administration of MTX in the treatment of psoriasis.     

前列腺素E_1脂微球注射液联合甘草酸二铵治疗重型肝炎疗效观察

目的:观察在综合治疗的基础上联合应用前列腺素E1脂微球(Lipo-PGE1)注射液和甘草酸二铵注射液治疗重型肝炎的疗效。方法:选取病毒型肝炎(乙型)、重型肝炎患者65例,在必要的综合治疗(对照组)基础上加用Lipo-PGE1注射液与甘草酸二铵注射液(治疗组)治疗4周,比较2组的临床疗效及生化指标。结果:与对照组比较,治疗组治疗2周、4周后临床症状明显减轻(P<0·01);治疗4周时总胆红素、凝血酶原时间、胆固醇、丙氨酸氨基转移酶显著好转(P<0·05),肝脏损害减轻。结论:在综合治疗基础上联用Lipo-PGE1与甘草酸二铵是治疗重型肝炎的有效方法。     

微针对白脉软膏经皮渗透性及应用安全性研究

目的 为改善白脉软膏经皮渗透性,采用微针技术进行促透,考察其对白脉软膏经皮渗透性和应用安全性的影响,以筛选安全、有效的微针应用条件。方法 以小鼠腹皮作为实验材料,采用改良的Franz扩散池进行体外渗透性评价,计算不同微针条件下白脉软膏3个主要成分甘草酸铵、甘松新酮、姜黄素的24 h累积经皮渗透量及稳态透皮速率,评估促透性能;采用光学相干断层扫描法（optical coherence tomography,OCT）记录微针处理后0、10、20、30、40、50、60、90、120min时的微孔道深度,进而对皮肤损伤及恢复程度进行量化评分,作为安全性评价依据。综合体外渗透性、皮肤损伤及恢复程度为评分指标（权重分别占40%、30%、30%）,运用正交试验设计将微针长度、作用力度和作用时间作为考察因素进行条件优化,筛选适宜的微针条件。结果 3个因素对综合评分的影响大小为微针长度>作用时间>作用力度,最终筛选出的微针条件为微针长度250μm、作用力度5 N、作用时间5 min。结论 综合考虑了微针对白脉软膏体外渗透性、皮肤损伤及恢复程度的影响,为白脉软膏及微针促透的临床合理安全应用提...     

基于AMPK/mTOR自噬通路研究甘草酸二铵对柯萨奇病毒B3病毒性心肌炎小鼠的保护作用

目的 基于单磷酸腺苷活化蛋白激酶（adenosine monophosphate-activated protein kinase,AMPK）/哺乳动物雷帕霉素靶蛋白（mammalian target of rapamycin,m TOR）自噬通路探究甘草酸二铵（diammonium glycyrrhizinate,DG）对病毒性心肌炎（viral myocarditis,VMC）小鼠心肌的保护作用及机制。方法 50只BALB/c小鼠随机分为对照组、模型组、DG(19.5mg/kg)组、DG(19.5 mg/kg)+Compound C(20 mg/kg)组和DG(19.5 mg/kg)+Compound C(20 mg/kg)+雷帕霉素（2.0 mg/kg）组,每组10只。采用试剂盒检测血清中肌酸激酶同工酶（creatine kinase-MB,CK-MB）活性、心肌钙蛋白Ⅰ（cardiac troponinⅠ,c TnⅠ）水平及心肌组织中白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平;...     

硫胺素治疗慢性乙型肝炎临床疗效观察

目的：观察硫胺素治疗慢性乙型肝炎的临床疗效。方法：64例慢性乙型肝炎患者，随机分成甘草酸二铵组32例，硫胺素组32例。在治疗第8周、第48周结束时分别检测肝功能、HBV DNA定性和HBeAg阴转情况。结果：甘草酸二铵组和硫胺素组肝功复常率在第8周两组间差异有显著性(P＜0．05)；血清HBVD门A、HBeAg转阴率两组间差异无显著性(P＞0．05)；硫胺素组与甘草酸二铵组比较肝功能复常率明显降低，差异有显著性(P＜0．01)。连续用药48周后，硫胺素组肝功能复常率、血清HBV DNA、HBeAg阴转率与治疗第8周结束时比较差异有显著性(P＜0．05)，肝功复常率两组间差异无显著性(P＞0．05)，血清HBV DNA、HBeAg阴转率硫胺素组与甘草酸二铵组比较差异有显著性(P＜0．05)。结论：硫胺素可作为慢性乙型肝炎的基本治疗药物，长期服用硫胺素可降低病毒含量，稳定肝脏内环境，促进肝功能恢复。     

甘草酸二铵治疗拉米夫定致HBV变异后的肝功能异常

目的:探讨甘草酸二铵治疗拉米夫定致HBV变异后的肝功能异常的疗效。方法:将1999年10月以来门诊使用拉米夫定治疗1年后发生病毒变异的40例患者,随机分为治疗组和对照组各20例,两组均接受一般保肝药治疗,治疗组在一般保肝治疗的基础上加用甘草酸二铵治疗,疗程8周。结果:治疗8周,肝功能恢复正常者,治疗组为15例(75%),对照组为9例(45%),治疗组疗效明显优于对照组(P<0.05)。结论:甘草酸二铵对治疗拉米夫定变异后的肝功能异常有较好的疗效。     

甘草酸单铵盐脂质体的试制及稳定性研究

采用薄膜水化超声-冷冻干燥法制备甘草酸单铵盐脂质体,建立RP-HPLC法测定脂质体含量及包封率,并从外观形态、粒径分布、含量、包封率等方面进行质量考察.所得制品平均粒径为146.4nm,包封率＞75%,初步稳定性实验表明在4℃密闭保存3个月,稳定性良好.