全文获取类型
收费全文 | 254981篇 |
免费 | 20478篇 |
国内免费 | 8219篇 |
专业分类
耳鼻咽喉 | 1707篇 |
儿科学 | 8192篇 |
妇产科学 | 1699篇 |
基础医学 | 22255篇 |
口腔科学 | 4491篇 |
临床医学 | 28153篇 |
内科学 | 64426篇 |
皮肤病学 | 3376篇 |
神经病学 | 27769篇 |
特种医学 | 6911篇 |
外国民族医学 | 5篇 |
外科学 | 19450篇 |
综合类 | 35507篇 |
现状与发展 | 38篇 |
一般理论 | 12篇 |
预防医学 | 20424篇 |
眼科学 | 2775篇 |
药学 | 18131篇 |
217篇 | |
中国医学 | 13254篇 |
肿瘤学 | 4886篇 |
出版年
2024年 | 798篇 |
2023年 | 5287篇 |
2022年 | 9270篇 |
2021年 | 13129篇 |
2020年 | 12498篇 |
2019年 | 9704篇 |
2018年 | 9478篇 |
2017年 | 9603篇 |
2016年 | 10073篇 |
2015年 | 9756篇 |
2014年 | 17794篇 |
2013年 | 19569篇 |
2012年 | 14806篇 |
2011年 | 16065篇 |
2010年 | 12658篇 |
2009年 | 12108篇 |
2008年 | 11994篇 |
2007年 | 11763篇 |
2006年 | 10432篇 |
2005年 | 8710篇 |
2004年 | 7383篇 |
2003年 | 6357篇 |
2002年 | 5441篇 |
2001年 | 4719篇 |
2000年 | 3928篇 |
1999年 | 3317篇 |
1998年 | 3119篇 |
1997年 | 2794篇 |
1996年 | 2507篇 |
1995年 | 2301篇 |
1994年 | 2107篇 |
1993年 | 1778篇 |
1992年 | 1677篇 |
1991年 | 1470篇 |
1990年 | 1173篇 |
1989年 | 1014篇 |
1988年 | 933篇 |
1987年 | 838篇 |
1986年 | 736篇 |
1985年 | 897篇 |
1984年 | 737篇 |
1983年 | 461篇 |
1982年 | 542篇 |
1981年 | 479篇 |
1980年 | 343篇 |
1979年 | 288篇 |
1978年 | 221篇 |
1977年 | 195篇 |
1976年 | 166篇 |
1975年 | 61篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
31.
目的 评价单克隆IgH基因重排检测在恶性淋巴瘤 (B NHL)临床中的应用价值。方法 用半巢式PCR检测单克隆IgH基因重排。病例组为B NHL ,包括 6 9例石蜡包埋组织切片、治疗前 16例骨髓和 2 9例外周血、阳性者治疗后复查骨髓和外周血 ;对照组为 10例慢性淋巴结炎、3例T NHL和 2例HD。结果 对照组均阴性。病例组 :切片中单克隆IgH基因重排阳性率为 6 3.8% (44 / 6 9) ;骨髓和外周血阳性率分别为 43 .8%(7/ 16 )和 41.4% (12 / 2 9) ,细胞形态学检查未见异常细胞者阳性率分别为 33 .3% (3/ 9)和 31.3% (5 / 16 )。 16例同时采集骨髓和外周血者 ,阳性率分别为 43 .8% (7/ 16 )和 37.5 % (6 / 16 ) ,两者无统计学差异。治疗前单克隆IgH重排阳性者 ,6例完全缓解 (CR)后转阴 ,处于持续缓解状态 ,1例临床缓解后 13个月仍阳性 ,现在继续随访中 ,另 1例CR后持续阳性者 ,6个月后复发。结论 切片、骨髓和外周血中检测单克隆IgH基因重排可以作为B NHL诊断和随访微小残留病灶的辅助手段 相似文献
32.
用酶联免疫吸附法对30例急性脑血管病(CVD)患者及32位正常人血清髓鞘碱性蛋白(MBP)含量进行检测。结果表明:急性CVD组患者血清MBP含量显著高于正常人组(P<0.01);血清MBP含量与急性CVD的严重程度相关。提示检测血清MBP含量对急性CVD诊断及预后判断有重要价值 相似文献
33.
本实验复制了莱姆病实验家兔模型,对血液生化23项进行了动态观察。结果表明,血液中谷丙转氨酶、γ-谷氨酰转肽酶、乳酸脱氢酶、谷草转氨酶、β-羟丁酸脱氢酶、磷酸肌酸激酶、胆固醇、尿素氮随病情加重而升高。葡萄糖、尿酸、磷随病情加重而减低。 相似文献
34.
Abstract Perioscan requires a plaque sample to detect the presence of enzymes capable of degrading N-benzoyl-DL-arginine-2-naphthylamide (BANA) from relatively few anaerobic periodontal pathogens. Periocheck assays the presence of neutral proteases in crevicular fluid. The aim of this study was to compare these test kits with traditional clinical methods of detecting periodontal disease and to monitor the ability of the kits to reflect the response to initial therapy. 19 patients with moderately severe chronic periodontitis were seen before and after a course of oral hygiene and root instrumentation consisting of 4 appointments. Clinical measurements and test assays were collected at 5 diseased sites and 2 healthy sites in each subject. Complete data from 125 sites were available for statistical analysis. At baseline Periocheck had a sensitivity of 88% and a specificity of 61% whereas Perioscan had a sensitivity of 99% and a specificity of 55%, when related to the clinical diagnosis. A composite clinical assessment, based on improvement or deterioration of one whole unit change of the subjective clinical indices and 2mm changes or greater in probing depth or probing attachment level, revealed 75 sites which improved following treatment, whereas 45 sites did not change and 5 sites deteriorated. The probability that the tests agreed with the clinical outcome after treatment, was calculated as 50.4% for Periocheck and 52% for Perioscan. The diagnostic kits did not reliably reflect the clinical assessment of periodontal disease in the cross sectional study, or the outcome following treatment. 相似文献
35.
Data on 232 members of a single pedigree, descended from two pairs of original parents, were made available to the participants of Genetic Analysis Workshop 8 (GAW8). In addition to information concerning age and sex, measurements for 10 quantitative traits and genotypes at 22 polymorphic marker loci were also provided for a subset of 193 of these family members. © 1993 Wiley-Liss, Inc. 相似文献
36.
Etiological heterogeneity in Hodgkin's disease: HLA linked and unlinked determinants of susceptibility independent of histological concordance 总被引:2,自引:0,他引:2
Forty-one multiplex families, from published sources and new data from the National Cancer Institute, segregating for Hodgkin's disease and HLA, have been studied. A reanalysis of these data strongly suggests a recessive mode of inheritance for susceptibility to Hodgkin's disease. The HLA haplotype sharing data between affected relatives demonstrate that approximately 60% of cases in multiplex families are due to an HLA-linked susceptibility gene, the remaining 40% being due to other familial factors. The data clearly support the hypothesis of etiological heterogeneity for Hodgkin's disease, with both HLA-linked and HLA-unlinked factors being responsible. Finally, there is an increased concordance of histological types between affected relatives, but this concordance seems independent of HLA sharing. 相似文献
37.
38.
Frederic T. Pender 《Journal of human nutrition and dietetics》1989,2(6):423-427
The study described tested the hypothesis that increasing amounts of dietary fibre (DF) in the diet of patients on haemodialysis (HD) may achieve positive clinical benefit without adversely affecting serum potassium and plasma phosphate. The current diet of 20 home HD patients was supplemented with 15g unprocessed wheat bran incorporated into three 'bran muffins' eaten daily for a trial period of 28 days. During this period patients reported an improvement in bowel habit. Serum potassium decreased slightly but not significantly ( P =0.242) but there was a significant rise in plasma phosphate ( P =0.004). These findings suggest that when increasing DF in devising HD dietary regimes, plasma phosphate is possibly the more sensitive biochemical variable following introduction of wheat bran. 相似文献
39.
R. N. KALARIA ‡‡ P. G. GALLOWAY† G. PERRY‡ ‡‡ 《Neuropathology and applied neurobiology》1991,17(3):189-201
Amyloid P (AP) component is present in all types of systemic amyloid deposits. Recently, it has been shown to be also present in cerebral amyloid lesions of Alzheimer's disease (AD). In this study, we used immunocytochemical methods to extend these findings at the electron microscope level and characterize the spectrum of AP immunoreactivity in neurofibrillary pathology (NFP) of AD and other neurodegenerative disorders including Down's syndrome (DS), Creutzfeldt-Jakob, Parkinson's, Pick's and diffuse Lewy body diseases and progressive supranuclear palsy. In AD and DS, AP immunoreaction product was evident in all the classical amyloid lesions and NFP in a large sample of all cortical areas examined. The distribution and relative intensity of immunostaining was similar to that of thioflavin S staining in serial sections. In many cases, however, plaques and vessels stained by anti-AP serum were not apparent with thioflavin S. Serial sections immunostained with antiserum to amyloid A, C-reactive protein or to other proteins involved in systemic amyloidoses and the acute phase response showed no evidence of staining in any of the cerebral lesions. Electron microscopy confirmed that AP immunoreactivity was associated with the abnormal filaments characteristic of NFP as well as amyloid fibrils found in plaques and vessels showing congophilic amyloid angiopathy. Plaques of Creutzfeldt-Jakob disease, Pick bodies of Pick's disease, tangles and Lewy bodies in Parkinson's disease and a subpopulation of Lewy bodies in the diffuse Lewy body disease coexistent with AD were also stained. With the exception of vessels in two of the five cases, AP was not detected in age-matched controls. Our observations indicate AP to be a consistent feature of cerebral NFP and amyloid deposits. 相似文献
40.
Neuropsychiatric disturbances are extremely common in Alzheimer’s disease (AD), and represent integral features of the illness,
as well as appropriate targets for therapy. We are interested in designing trials aimed at preventing or delaying the emergence
of psychopathology in AD. For symptomatic treatment of agitation, mood stabilizers, particularly sodium valproate, have proved
to be beneficial in some patients. Since these effects take several weeks to emerge, we considered that they might be dependent
on potentially neuroprotective actions of valproate, such as inhibition of apoptosis and slowing of neurofibrillary tangle
formation. In this article we present the rationale for testing the neuroprotective potential of valproate experimentally
in mouse models of tauopathy and in a clinical trial of patients with AD who lack psychopathology at baseline. Together, these
studies will provide important tests of the hypothesis that valproate, either through inhibition of tau phosphorylation or
some other mechanism, is a useful therapeutic agent to modify disease progression in AD. 相似文献