工厂两级计算机系统之间的通讯

论述了IBM-PC及其兼容机之间的点点通讯,加装调制解调器通讯距离可达数公里。采用该技术可完成厂部与车间两级计算机系统的分级管理与控制。     

Contribution of Monoamine Oxidase(MAO) to the Binding of Tertiary Basic Drugs in Isolated Perfused Rat Lung

The effect of tertiary basic drugs on mitochondrial MAO activity and the effect of MAO inhibitors (MAOIs) on basic drug accumulation in the isolated perfused rat lung were studied to clarify the role of MAO in drug binding to lung tissue. In the perfused lung preparation, the inhibition of MAO by basic drugs correlated well with their lipid solubilities and followed competitive kinetics. The inhibitory rank order (imipramine diphenhydramine > quinine > metoclopramide > procainamide) also correlated with their accumulation in the perfused lung. Moreover, MAOI treatment decreased the accumulation of basic drugs in the lung, and the potency of MAOIs to inhibit drug accumulation in the lung correlated with their MAO inhibitory activity. These results indicate that lung MAO has specific binding sites for basic drugs and may function as a drug reservoir.     

Survival of Purkinje Cells in Cerebellar Cultures is Increased by Insulin-like Growth Factor I

Insulin-like growth factor I (IGF-I) is a trophic factor for both neurons and glia. Its presence in the developing and adult cerebellum suggests a role for this growth factor in this area of the brain. Recently, we have described the existence of an IGF-I-containing pathway in afferents of Purkinje neurons arising from the inferior olive. In addition, IGF-I receptors are present in the molecular layer of the cerebellar cortex. These observations prompted us to investigate whether the Purkinje cell is a target for IGF-I. Addition of IGF-I to rat cerebellar cultures produced a 7-fold increase in the number of Purkinje cells (calbindin-positive) together with an increase in the calbindin content of the cultures. IGF-I also doubled the number of surviving neurons and produced a moderate, non-significant increase in [³H]thymidine incorporation by the cultures. On the other hand, basic fibroblast growth factor (bFGF), which is also present in the cerebellum, produced a dramatic increase in both the proportion of astrocytes and in the mitotic activity of the cultures, without affecting neuron survival. We conclude that IGF-I is a specific promoter of Purkinje cell survival and that its effects differ from those produced by bFGF in fetal cerebellar cultures. These findings reinforce our hypothesis that the Purkinje cell is a target neuron for IGF-I action in the developing cerebellum.     

Regulation of the biosynthesis and processing of chromogranins in organotypic slices: influence of depolarization, forskolin and differentiating factors

Slices from rat hippocampus in organotypic culture were used to study the biosynthesis regulation of chromogranins A and B and secretogranin II. Additionally, we investigated the proteolytic conversion of secretogranin II and the levels of prohormone convertases putatively involved. Forskolin treatment and depolarization with potassium plus BayK 8644 led to significant increases in secretogranin II mRNA in the principal cells of the hippocampus. Enhanced expression of secretogranin II was also reflected by a rise in peptide levels. Despite this induction of biosynthesis the extensive processing to secretoneurin normally observed in brain was maintained. Both forskolin and depolarization upregulated the prohormone convertase (PC)1, but not PC2, indicating that PC1 levels are critical for secretoneurin production under stimulating conditions. Results obtained for chromogranins A and B were less consistent. For chromogranin A mRNA, changes were restricted to granule cells; for chromogranin B, a response in granule cells was observed to depolarization but not to forskolin, and effects in pyramidal neurons were weak. Accordingly, we were unable to detect alterations in chromogranin A and B protein levels. Furthermore, we tested several neurotrophic growth factors and found that only basic fibroblast growth factor raised secretogranin II expression without affecting chromogranins A and B. The hippocampal slice preparation allowed well controlled treatment with identification of neuronal subpopulations and yielded data largely matching experiments in vivo and in cell culture. The pronounced regulation of secretogranin II and its effective processing underlines the importance of the resulting peptide secretoneurin as an active neuropeptide in the nervous system.     

碱性成纤维细胞生长因子对胎鼠宫内窘迫脑损害的疗效观察

目的探讨母鼠应用碱性成纤维细胞生长因子（ｂＦＧＦ）是否对胎鼠宫内窘迫脑损害有保护作用。方法将５３只胎龄为２０天的ＳＤ大鼠胎鼠随机分为五组，即正常对照组（Ｃ组）１１只，窘迫对照组（Ｅ组）１０只，ｂＦＧＦ治疗组Ⅰ（Ｄ１组）１０只，ｂＦＧＦ治疗Ⅱ组（Ｄ２组）１０只，ｂＦＧＦ治疗Ⅲ组（Ｄ３组）１２只。在不同时间内静脉注射ｂＦＧＦ，监测静脉注射ｂＦＧＦ后胎鼠脑细胞内外钙、钠、钾含量的变化。结果在钳夹血管前２０分钟静脉注射ｂＦＧＦ后胎鼠脑细胞胞游离钙离子浓度为４７５±９１ｎｍｏｌ／Ｌ；再灌注同时静脉注射ｂＦＧＦ后游离钙离子浓度为４７４±４９ｎｍｏｌ／Ｌ。表明ｂＦＧＦ干预组均明显高于正常对照组（３１５±８７ｎｍｏｌ／Ｌ）（Ｐ＜００１），但亦均低于窘迫对照组（５５２±９４ｎｍｏｌ／Ｌ）（Ｐ＜００５）；同时这两组的脑组织总钙、总钠及总钾含量亦有不同程度的下降。结论无论是在窘迫前还是在缺血再灌注同时静脉注射ｂＦＧＦ，ｂＦＧＦ均可减轻宫内窘迫胎鼠脑细胞钙超载，减轻脑水肿，保护缺氧脑细胞；提示对产妇或窒息新生儿应用ｂＦＧＦ可能有助于减轻窒息新生儿脑损伤     

Influence of intratumoral basic fibroblast growth factor concentration on survival in ovarian cancer patients

Since basic fibroblast growth factor (bFGF) is considered as a potent mitogen that stimulates the growth of ovarian cancer cells, we evaluated the role of bFGF as a prognostic marker in patients with epithelial ovarian cancer. bFGF was quantified from the tumor cytoplasm of 76 patients with FIGO stage I–III ovarian cancer by a human FGF basic immunoassay (R&D Systems). After a mean follow-up period of 42 months, 50 patients were found to be free of tumor while 26 patients had died of the disease. The median bFGF concentration was 352.9 pg/mg (range 27.4–26 600 pg/mg). After dichotomization cytoplasmic expression of bFGF was found to be low in 44 tumors (≤500 pg/mg) and high in 32 tumors (>500 pg/mg). The probability of overall survival was 38.8 and 58.5% in the low bFGF and high bFGF groups, respectively (log-rank P=0.0066). In multivariate analysis, residual tumor after initial surgery and bFGF, but not histologic grade or stage of the disease, independently influenced the overall survival probability. Furthermore, tumors with high cytoplasmic expression of bFGF revealed a much greater stromal content. Therefore, we hypothesize that bFGF may induce a fibroblastic response which causes tumors with a high bFGF to be less aggressive than those with less stromal tissue.     

社区卫生服务及基本卫生服务主要内容探讨

本文分两部分：第一部分主要介绍了国内外社区卫生服务的发展模式及主要内容；第二部分主要介绍了国外基本卫生服务的界定原则、界定方法及主要内容。     

对医学类高职高专院校计算机基础教育的思考

探讨了目前医学类高职高专院校计算机教育问题,通过分析医学高职高专院校计算机教学的目的和现状,对解决目前存在的一些问题做了初步思考.     

碱性成纤维细胞生长因子对新生鼠缺氧缺血性脑损伤胶质纤维酸性蛋白表达的影响

目的探讨外源性碱性成纤维细胞生长因子（bFGF）对新生大鼠缺氧缺血性脑损伤（HIBD）后海马胶质纤维酸性蛋白（GFAP）表达的影响。方法通过结扎并剪断7日龄新生Wistar大鼠右侧颈总动脉，吸入8％氧气和92％氮气2h制备新生鼠HIBD模型，设假手术组、生理盐水对照组、bFGF治疗组。通过免疫组织化学方法和计算机图像分析技术检测3组大鼠不同时点（术后d4、7、10、17、24）海马CAI区GFAP表达强度变化。结果假手术组海马内GFAP阳性细胞数量和染色强度术后d7达高峰：对照组、治疗组GFAP表达较假手术组增多，治疗组增多更明显，术后d10达高峰，GFAP阳性细胞主要分布于海马CAI、CA3区．术后d4、10、17组比较差异有显著性（P均〈0．05）。结论1．新生鼠HIBD后脑缺血易损伤区GFAP表达增加，可能与脑损伤后神经细胞再生有关；2．外源性给予bFGF可增强新生鼠脑缺氧缺血后中枢神经系统GFAP表达，在神经细胞损伤的修复中发挥一定保护作用。