Immunohistochemical study of the distribution of sodium-dependent vitamin C transporters in adult rat brain

Sodium-dependent vitamin C transporters (SVCTs) is known to transport the reduced form of ascorbic acid into the cell, whereas the oxidized form of vitamin C (VC) is moved through a facilitative sugar transporter, such as glucose transporter (GLUT). With regard to the distribution of SVCT1 and -2 within the various organs, they were reported to be expressed in different types of cells. Especially in the central nervous system, only SVCT2 mRNA was expressed mainly in neurons and some types of neuroglial cells. However, data on the expression of SVCT proteins in the brain are scant. Therefore, we tried to develop comprehensive data on the distribution of SVCT proteins in adult rat brain by using immunohistochemical techniques for the first time. In our study, SVCT2 immunoreactivities (IRs) were intensely localized in the neurons of cerebral cortex, hippocampus, and Purkinje cells of cerebellum, and much weaker SVCT2 IRs were found in the other brain regions. Judging from double-immunohistochemical data, most of the cells expressing SVCT2 IRs were likely to be neurons or microglia, even though the cells in choroids plexus or ependymal cells around the ventricles also exhibited SVCT2 IRs. Complete mapping of the distribution of SVCT2 IRs was available by using a semiquantitative method. The subcellular localization of SVCT proteins is necessary for understanding the exact role of the protein, so the current overall mapping of SVCT IRs in the rat brain could be the basis for further studies on related subjects.     

低分子肝素联合环磷酰胺治疗特发性间质性肺炎的临床研究

目的探讨低分子肝素联合环磷酰胺治疗特发性间质性肺炎的临床疗效。方法选取天津市海河医院2014年10月—2015年10月收治的特发性间质性肺炎患者60例,随机分为对照组(30例)和治疗组(30例)。对照组患者口服环磷酰胺片,25 mg/次,1次/d,每周增加25 mg,最大量150 mg/d,每治疗2周休息2周;治疗组治疗组在对照组的基础上皮下注射低分子肝素钙注射液,5 000 U/次,2次/d,每个月注射前两周,两组均治疗4个月。治疗后,观察两组临床–影像–生理评分(CRP)评分、动脉血氧分压(pO_2)和肺活量(VC)、血浆白介素-6(IL-6)和血清肿瘤坏死因子-α(TNF-α)水平以及治疗中不良反应发生情况。结果治疗后,两组的临床评分和生理评分均比治疗前显著降低(P0.05),且治疗组降低的更为明显,两组比较差异具有统计学意义(P0.05)。两组患者pO_2和VC均有所提高,同组治疗前后比较差异具有统计学意义(P0.05)。且治疗后治疗组的pO_2和VC明显高于对照组,两组比较差异具有统计学意义(P0.05)。两组的IL-6和TNF-α均较治疗前显著降低(P0.05),且治疗组较对照组降低更为明显,两组比较差异具有统计学意义(P0.05)。治疗组和对照组不良反应率分别为3.33%、16.67%,两组比较差异具有统计学意义(P0.05)。结论低分子肝素联合环磷酰胺治疗特发性间质性肺炎患者临床效果显著,且不良反应少,具有一定的临床推广应用价值。     

平均血小板体积与精索静脉曲张中炎症病理过程的相关性

目的探讨平均血小板体积(MPV)与精索静脉曲张炎症病理过程的相关性,以研究精索静脉曲张的发病机制。方法选取2013年1月至2019年1月在东莞市妇幼保健院诊治的83例精索静脉曲张患者作为研究对象。将这83例患者设为精索静脉曲张(VC)组。随机挑选健康男性101例设为对照组。比较两组MPV值,以受试者工作曲线(ROC)分析MPV对精索静脉曲张的诊断价值,用Spearman等级相关检验分析MPV在精索静脉曲张中炎症病理过程中的相关性。结果精索静脉曲张组MPV值高于对照组,两组间差异具有统计学意义(P<0.05)。ROC分析MPV的最佳截断值为9.75 fL。MPV与精索静脉曲张分级以及静脉曲张直径呈正相关。结论 MPV在精索静脉曲张组中的水平高于对照组,并且与曲张静脉的直径以及曲张的分级程度呈正相关趋势,MPV对于精索静脉曲张炎症病理机制的研究具有重要的意义。     

Heart rate variability during extracorporeal membrane oxygenation and recovery in severe neonatal disease

BACKGROUND: Heart rate variability (HRV) reveals information on the functional state of the autonomic nervous system (ANS) in neonates. During severe illness, heart rate variability is impaired. AIM: This study was initiated to measure the changes in HRV in neonates during extracorporeal membrane oxygenation (ECMO) and recovery from severe respiratory and circulatory failure. Moreover, we compared our data with HRV data of healthy newborns and we investigated the differences in HRV parameters between ECMO-survivors and non-survivors. STUDY DESIGN: This study is of an observational character. We performed short-term recordings of heart rate variability in 14 neonates during ECMO and recovery. We computed time- and frequency-domain HRV parameters. RESULTS: ECMO significantly affects time-domain HRV parameters. Severe neonatal illness causes a significant reduction of all calculated HRV parameters; clinical recovery is accompanied by an increase of HRV. In comparison with normative data of healthy newborns, however, HRV remains impaired. The ECMO-development ratio separated the non-survivors from the survivors during ECMO therapy. CONCLUSIONS: During severe neonatal illness, HRV is impaired. It remains to be clarified whether the impairment of HRV during severe illness can predict the neurological outcome. The ability of the E/D ratio as an HRV parameter to serve as a predictive tool has to be corroborated in larger group of patients.     

酸处理对采后香菇保鲜的影响

采用柠檬酸结合抗坏血酸对贮藏期间香菇进行保鲜研究,结果表明,柠檬酸结合抗坏血酸处理香菇,可有效抑制香菇呼吸强度的升高,抑制香菇失重率的增加,降低采后香菇细胞膜透性,减少香菇贮藏期抗坏血酸的损失;在4℃下采用0.05g/dLVC+0.10g/dL柠檬酸处理,可以较好地保鲜香菇,使香菇在10d内保持较好的品质.酸处理对香菇两种装载量包装薄膜袋中氧气和二氧化碳体积分数有一定的影响,装载量小的薄膜袋内,可以保持合适的氧气和二氧化碳体积分数,而装载量大的薄膜袋包装则相反.装载量小的薄膜袋包装更有利于香菇的保鲜.     

重组人生长激素治疗生长激素缺乏症对肺功能的影响

目的研究重组人生长激素(rhGH)治疗生长激素缺乏症(GHD)对肺功能的影响。方法10例GHD伴促性腺激素缺乏的男性患儿,年龄15.6±3.2岁,身高年龄(HA)8.82±1.81岁,给予rhGH(健高宁)治疗,剂量为0.11U·kg     

维生素C对神经胶质瘤的影响作用

神经胶质瘤是中枢神经系统(CNS)最常见的一类肿瘤,占了颅内原发肿瘤的35%～60%。2007年世界卫生组织(WHO)中枢神经系统肿瘤分类中将胶质瘤分为I～Ⅳ级,其中Ⅲ、Ⅳ级为恶性胶质瘤,大约占所有胶质瘤的77.5%。目前对神经胶质瘤的临床治疗采取以手术治疗为主,结合放疗、化疗等疗法的综合治疗,虽然能延长患者生存时间,但存在高复发率、高致残率、高病死率等问题,总体预后仍然较差。自20世纪70年代以来,维生素C(VC)抗肿瘤作用的研究日益进展,国外的医疗工作者已将其作为肿瘤治疗的补充和替代疗法之一,并报道了静脉注射维生素C治疗神经胶质瘤、卵巢癌、肾细胞癌、乳腺癌、大肠癌、非霍奇金淋巴瘤、前列腺癌、膀胱癌等肿瘤的临床病例,认为其能改善患者的临床症状、提高生存质量、延长生存期。本文就维生素C对神经胶质瘤的治疗作用做一综述。