Total nucleated cell counts are driving clinician's choice rather than cryopreservation period: Lesson for cord blood banks

According to the increase in both the number of cryopreserved cord blood (CB) units and the cryopreservation period for each CB unit in the largest public CB bank in Korea, we are pursuing greater efficiency in CB bank management. Thus, we analyzed whether the cryopreservation period has a negative impact on the selection of CB units for CB transplantation (CBT). Until December 2019, 468 CB units were used for transplantation. The cryopreservation period, total nucleated cell (TNC), and CD34+ cell counts were analyzed among the CB units according to the CBT-year and the donation year. The results showed that the cryopreservation period was increased in recent CBT-year groups. The transplanted CB units showed similar TNC counts irrespective of the donation year, and the mean TNC count was 13.9 × 10⁸/unit. CB units cryopreserved for a relatively long period were transplanted consistently. The mean TNC count of CB units cryopreserved for over 10 years was 16.4 × 10⁸/unit. The mean CD34+ cell counts were not significantly different among the CB units transplanted after CBT-2013 and among the CB units donated after CBT-2011. Through an analysis of the CB units selected by clinicians for CBT, this study revealed that clinicians placed more weight on the TNC counts than on the cryopreservation period of cryopreserved CB units. Therefore, the minimum TNC count of CB units suitable for cryopreservation should be increased up to 13.0 × 10⁸/unit to balance the satisfaction of clinicians’ needs with the efficiency of the CB bank.     

组配式股骨假体髋关节翻修治疗在全髋关节置换术后股骨假体周围骨折中的应用效果分析

目的 回顾性分析组配式股骨假体髋关节翻修治疗在全髋关节置换术后股骨假体周围骨折中的应用效果。方法 回顾性选取2018年4月至2019年4月上海市第八人民医院收治的股骨假体周围骨折患者60例作为研究对象。其中30例患者采用骨水泥假体髋关节翻修治疗,设定为对照组,采用组配式股骨假体髋关节翻修治疗的30例患者设定为观察组,并随访6个月。观察两组患者手术情况、手术前后哈里斯髋关节评分(Harris hip score,HHS)及帕克-帕尔莫移动评分(Parker Palmer mobility score,PPMS)、手术前后VAS评分及骨折愈合情况。结果 所有患者手术顺利,且无发生特殊并发症及不良事件。观察组手术时间、术中出血量、输血量、住院时间[(65. 12±22. 13) min、(1 986. 52±875. 63) ml、(955. 25±520. 17) ml、(12. 98±5. 11) d]显著优于对照组[(76. 38±24. 64) min、(2 437. 42±953. 87) ml、(1 226. 44±553. 09) ml、(15. 52±5. 83) d],差异有统计学意义(P <0. 05)。观察组术后HHS及PPMS评分分别为(73. 66±16. 05)分,(8. 37±2. 13)分,显著优于术前的(4. 46±1. 34)分、(0. 83±0. 27)分,差异具有统计学意义(P<0. 05);观察组术后HHS评分及PPMS评分显著优于对照组的(66. 42±14. 83)分、(7. 15±1. 96)分,差异有统计学意义(P <0. 05);观察组患者的骨折愈合时间为(14. 29±6. 3)周,与对照组的(15. 03±7. 5)周相比差异无统计学意义(P> 0. 05)。治疗后观察组患者的VAS评分为(2. 86±0. 99)分,显著优于对照组的(3. 73±1. 28)分,差异具有统计学意义(P <0. 05)。结论 组配式股骨假体髋关节翻修治疗全髋关节置换术后股骨假体周围骨折,术后患者HHS和PPMS评分明显提高,VAS评分明显降低,临床效果比较满意,值得临床推广应用。     

围术期康复护理对髋关节置换术患者睡眠状态及髋关节功能的影响

目的:研究围术期康复护理对髋关节置换术患者髋关节功能及睡眠状态的影响.方法:选取2016年8月至2018年10月福建医科大学附属三明第一医院骨科收治的髋关节置换术患者90例作为研究对象,随机分为观察组和对照组,每组45 例.分别进行系统化和常规康复护理.比较2组术前和术后匹兹堡睡眠质量指数量表(PSQI)和Harris评分.结果:与术前比较,术后2组Harris评分显著提高,观察组患者术后1,3,6个月髋关节功能评分均高于对照组(P<0.05).与术前比较,术后2组PSQI评分降低,观察组PSQI评分低于对照组(P<0.05).结论:系统化康复护理更有利于髋关节置换术后患者髋关节功能的康复以及睡眠质量的提高,具有重要的临床价值.     

Large pelvic mass arising from the cervical stump: A case report

BACKGROUND A large cervical cyst with a cervical high-grade squamous intraepithelial lesion arising from the cervical stump is rare.After supracervical hysterectomy,there is a risk of various lesions occurring in the cervical stump.We review the types and characteristics of cervical stump lesions and compare total hysterectomy with subtotal hysterectomy.Gynecologists should choose the most suitable surgical method based on both the patient’s condition and wishes.If the cervix is retained,patients require a close follow-up.CASE SUMMARY A 57-year-old woman was admitted to the Gynecology Department for a large pelvic mass.Her chief complaint was abdominal distention for two months.She had undergone subtotal supracervical hysterectomy for leiomyoma 14 years prior.Abdominal ultrasonography detected a 9.1 cm×8.5 cm×8.4 cm anechoic mass with silvery fluid in the pelvic cavity and high-risk human papilloma virus 53(HPV53)was positive.The admission diagnosis we first considered was a pelvic mass mimicking carcinoma of the cervical stump.We performed a laparotomy and a rapid frozen biopsy was suggestive of a fibrous cyst wall coated with a high squamous intraepithelial lesion.The pelvic mass was removed,and a bilateral adnexectomy was implemented.Final pathology confirmed that the pelvic mass was a large inflammatory cyst with a cervical high-grade squamous intraepithelial lesion.After successful intervention,the patient was discharged one week after surgery and there was no recurrence of the vaginal stump at 43 mo.CONCLUSION When addressing benign uterine diseases,gynecologists should pay adequate attention to retaining the cervix.If the cervix is retained,patients require a close follow-up.     

TIMELINE OF GAINS IN QUADRICEPS STRENGTH SYMMETRY AND PATIENT-REPORTED FUNCTION EARLY AFTER ACL RECONSTRUCTION

Background:Quadriceps weakness is a predictor of long-term knee function and strength recovery can vary from months to years after anterior cruciate ligament reconstruction (ACLR). However, few studies evaluate quadriceps strength and self-reported function within the first several weeks after ACLR.Hypothesis/Purpose:To examine changes over time in quadriceps strength symmetry, quadriceps peak torque, and self-reported knee function prior to and at six, 12, and 24 weeks post-ACLR. The hypotheses were 1) quadriceps strength symmetry, bilateral quadriceps peak torque, and patient-reported function would improve over time from pre-ACLR to 24 weeks post-ACLR and 2) significant improvements in patient-reported function, but not strength symmetry, would occur between time points.Study Design:Prospective, cohort studyMethods:Thirty participants completed four testing sessions: pre-surgery and six, 12, and 24 weeks post-ACLR. Isometric quadriceps strength testing was performed at six weeks and isokinetic quadriceps strength was measured at all other testing points. Quadriceps index was calculated to evaluate between limb quadriceps strength symmetry. The Knee injury and Osteoarthritis Outcome Score (KOOS) and International Knee Documentation Committee Subjective Knee Evaluation Form (IKDC) were administered at each time point. A repeated-measures analysis of variance evaluated changes over time, with post-hoc comparisons to determine at which time-point significant changes occurred.Results:Quadriceps strength symmetry, involved limb quadriceps peak torque and all patient-reported outcome scores increased over time (p<0.02). Post-hoc tests showed that neither self-reported outcomes, nor quadriceps index improved between pre-surgery and six-weeks post-ACLR. From six to 12 weeks post-ACLR, scores on IKDC and KOOS Pain, Symptoms, Quality of Life, and Sport subscales improved (p≤0.003). From 12 to 24 weeks post-ACLR, quadriceps strength symmetry, involved limb quadriceps peak torque, KOOS-Symptoms, Quality of Life, and Sport subscales and the IKDC improved (p≤0.01). Uninvolved limb quadriceps peak torque did not change across any time point (p≥0.18).Conclusion:Patient-reported knee function increased between six and 24 weeks post-ACLR, while increases in involved limb quadriceps strength and quadriceps strength symmetry were not noted until 12-24 weeks post-ACLR.Level of Evidence:2b, individual cohort study     

早期营养支持对急性重症胰腺炎病人机体组成的影响

目的:探讨早期肠内营养(EN)联合肠外营养(PN)对急性重症胰腺炎(ASP)病人机体组成的影响. 方法:将30例病人随机分成治疗组和对照组.治疗组病人采取早期EN加PN,并逐渐减少PN的用量,直至停止PN.对照组病人采取完全胃肠外营养(TPN).两组病人平均治疗15 d.用皮皱测量法测定两组病人机体密度,用MRI方法测量全身各部位脂肪变化情况,核素稀释法测定全身水量(TBW)、总脂肪量(TBF)、无脂肪体重(LBM)、细胞内液(ICM)、细胞外液(ECM)的变化.同时常规测定血清清蛋白、血红蛋白、转铁蛋白和淋巴细胞数量等. 结果:治疗组在TBW、TBF、LBM、ICM、ECM、血清清蛋白、血红蛋白、转铁蛋白与对照组差异无显著性意义(P＞0.05),治疗组的淋巴细胞数高于对照组(P＜0.05). 结论:早期EN联合PN可以改善ASP病人的机体免疫功能,对机体组成的影响,与TPN无差异.     

某市三所医院《国家基本医疗保险药品目录》使用现状分析

目的:通过对三所医院的医保患者的住院用药分析,探讨《国家基本医疗保险药品目录》的使用情况及存在的问题,并对相关政策提出建议. 方法:收集了2004-07/12某市三所大型综合性医院所有参保患者用药数据,并按药品所属类型和病种用药分类统计. 结果:三所医院六类药物与五种常见病《药品目录》内外药品费用情况存在差异(P《0.05). 结论:医保患者药费结构需要进一步调整或应进一步控制自付药费比例,以减轻参保职工的经济负担.     

公立医院基层党建考核体系的构建与实践

目的构建“党员—党支部书记—党支部工作”为一体的基层党建考核体系,推动公立医院基层党建质量持续提升。方法党员实施“100分+20分”积分量化考核,党支部书记实施“1+1”述职评议考核,党支部工作实施“532”量化考核,注重三者考核结果运用的深度融合。结果2020年党员积分量化考核中,总分100分以上者占比由2019年5...     

妇幼保健机构信用等级评价指标体系的构建

目的建立适用于我国妇幼保健机构的信用等级评价指标体系,为推进妇幼保健机构信用等级评价工作提供参考。方法综合运用文献分析法、专家咨询访谈、课题组研讨等方式构建初步指标体系,通过两轮德尔菲专家调查形成最终指标并赋予权重。结果建立了主要包含7个一级指标、38个二级指标的妇幼保健机构信用等级评价指标体系。结论研究建立的妇幼保健...     

“系统疾病诊断小组”工作模式对检验医师和技师能力提升的实践与思考

为了提高检验科工作人员的临床思维和检测结果的临床解读能力,本研究提出了一种新的工作模式,即“系统疾病诊断小组”模式。该模式借鉴“器官导向教学”和“PBL教学”的指导思想,组织科室检验医师和具有临床医学背景的检验技师从学习疾病诊疗指南和典型案例诊断路径入手,通过多种途径和形式参与临床沟通和合作。该模式的目的是打破检验亚专业界限,强调检验专业知识的融会贯通和相关临床诊疗知识的学习积累,最终具备将检验结果有效转换为诊疗信息的能力。该工作模式已经运行了2年多,科室陆续成立了8个系统疾病诊断小组,小组成员的临床诊疗参与意识和能力得到了显著提升。受邀参加临床会诊和加入多学科诊疗模式（multiple disciplinary team,MDT）团队的人次较前明显增多,并尝试创建具有诊疗意义的检验分析报告和疾病临床诊断检验路径等,受到临床广泛好评。然而,在运行过程中,仍存在需要制定小组成员个性化培养计划和完善系统疾病诊断小组工作制度和管理制度等问题。