疏络化纤颗粒的质量标准研究

目的:建立疏络化纤颗粒的质量控制方法。方法:对制剂中的百合、灵芝、茜草、地龙、丹参采用薄层色谱(TLC)法进行定性鉴别;采用高效液相色谱(HPLC)法对制剂中的疏络化纤颗粒所含成分芍药苷进行含量测定,以C18柱为色谱柱,乙腈-0.1%磷酸水溶液(1486)为流动相,检测波长为230 nm。结果:用薄层鉴别百合、灵芝具有较好的专属性;在0.313~0.626μg范围内芍药苷与峰面积呈良好的线性关系,r2=0.9999,回收率均在95%与105%之间,RSD小于5%(n=6)。结论:所建立的方法专属性强,简便准确,该制剂标准可有效控制其质量。     

Localization and variable expression of G alpha(i2) in human endometrium and Fallopian tubes

BACKGROUND: Heterotrimeric G proteins take part in membrane-mediated cell signalling and have a role in hormonal regulation. This study clarifies the expression and localization of the G protein subunit G alpha(i2) in the human endometrium and Fallopian tube and changes in G alpha(i2) expression in human endometrium during the menstrual cycle. METHODS: The expression of G alpha(i2) was identified by Polymerase chain reaction (PCR), and localization confirmed by immunostaining. Cyclic changes in G alpha(i2) expression during the menstrual cycle were evaluated by quantitative real-time PCR. RESULTS: We found G alpha(i2) to be expressed in human endometrium, Fallopian tube tissue and in primary cultures of Fallopian tube epithelial cells. Our studies revealed enriched localization of G alpha(i2) in Fallopian tube cilia and in endometrial glands. We showed that G alpha(i2) expression in human endometrium changes significantly during the menstrual cycle, with a higher level in the secretory versus proliferative and menstrual phases (P < 0.05). CONCLUSIONS: G alpha(i2) is specifically localized in human Fallopian tube epithelial cells, particularly in the cilia, and is likely to have a cilia-specific role in reproduction. Significantly variable expression of G alpha(i2) during the menstrual cycle suggests G alpha(i2) might be under hormonal regulation in the female reproductive tract in vivo.     

诺舒子宫内膜去除术治疗子宫腺肌病致月经过多的临床研究

目的探讨针对子宫腺肌病致月经过多患者采用诺舒(NovaSure)子宫内膜去除术治疗的临床意义。方法选取2012年3月至2013年6月因子宫腺肌病在番禺中心医院行NovaSure子宫内膜去除术的患者29例为研究组,行宫腔镜下子宫内膜电切术的患者27例为对照组,所有患者术后门诊定期随访,根据月经失血评估图(pictorial blood loss assessment chart,PBAC)评分判定术前出血情况、手术时间、术中出血量、术后闭经率、月经减少率和月经改善率。结果研究组手术时间、术中出血量少于对照组(P0.05);两组术后并发症率、治疗有效率比较差异无统计学意义(P0.05)。结论 NovaSure子宫内膜去除术是治疗子宫腺肌病致月经过多的有效方法。     

A novel multivariate approach using science-based calibration for direct coating thickness determination in real-time NIR process monitoring

This study demonstrates an approach, using science-based calibration (SBC), for direct coating thickness determination on heart-shaped tablets in real-time. Near-Infrared (NIR) spectra were collected during four full industrial pan coating operations. The tablets were coated with a thin hydroxypropyl methylcellulose (HPMC) film up to a film thickness of 28 μm. The application of SBC permits the calibration of the NIR spectral data without using costly determined reference values. This is due to the fact that SBC combines classical methods to estimate the coating signal and statistical methods for the noise estimation. The approach enabled the use of NIR for the measurement of the film thickness increase from around 8 to 28 μm of four independent batches in real-time. The developed model provided a spectroscopic limit of detection for the coating thickness of 0.64 ± 0.03 μm root-mean square (RMS). In the commonly used statistical methods for calibration, such as Partial Least Squares (PLS), sufficiently varying reference values are needed for calibration. For thin non-functional coatings this is a challenge because the quality of the model depends on the accuracy of the selected calibration standards. The obvious and simple approach of SBC eliminates many of the problems associated with the conventional statistical methods and offers an alternative for multivariate calibration.     

稀碱法提取金线莲多糖的研究

目的：研究稀碱法提取金线莲多糖的最佳条件。方法采用DNS法测定多糖含量,以多糖含量为指标,对金线莲多糖的提取沉淀过程中的影响因素进行考察。结果稀碱法提取金线莲多糖的最佳条件是提取温度70℃、提取时间1.5h、料液比1∶35、提取次数2次,静置时间5h、离心转数7000r· min -1、离心时间5min。结论稀碱法提取金线莲多糖是一种效率高且稳定可行的提取方法。     

腋窝皱纹切口薄皮瓣法负压引流根治术与皮下搔刮术治疗腋臭的临床对比研究

目的 比较腋窝皱纹切口薄皮瓣法负压引流根治术与皮下搔刮术治疗腋臭的疗效及安全性.方法 选取2005年12月～2013年10月收治的286例腋臭患者分为两组,其中薄皮瓣组144例采用腋窝皱襞切口薄皮瓣法负压引流根治术治疗,皮下搔刮组142例采用皮下搔刮术治疗,术后随访6～12个月,比较两种术式的疗效及并发症情况.结果 薄皮瓣组和皮下搔刮组的总有效率分别为100.00％、88.03％ （P＜0.01）,复发率分别为4.86％、17.61％（P=0.001）.两组的皮下血肿、切口感染发生率比较差异无统计学意义（P＞0.05）;两组的异味残留、瘢痕及皮肤坏死发生率比较差异有统计学意义（P＜0.05）.结论 两种术式均为治疗腋臭的有效方法,但腋窝皱襞切口薄皮瓣法负压引流根治术疗效好,术后并发症少,值得临床推广应用.     

药物流产不全清宫后服用屈螺酮炔雌醇片的临床疗效评价

目的探讨药物流产不全清官术后即日口服屈螺酮炔雌醇片的临床疗效。方法将64例药物流产不全清宫后患者随机分为两组,观察组32例．在预防感染及促进子宫收缩的基础上于清宫后即日口服屈螺酮炔雌醇片,连续服用21d;对照组32例,仅术后口服同剂量同种抗生素预防感染及促进子宫收缩3d。结果观察组子宫内膜恢复明显优于对照组（P〈0．05）,出血量明显减少,出血时间明显缩短,月经恢复快,宫颈管粘连及官腔粘连的发生率低。结论药物流产不全清宫后即日口服屈螺酮炔雌醇片可以促进子宫内膜修复,月经复潮快,可降低药物流产不全清宫后并发症的发生率,可作药物流产清宫后的常规用药。     

Liquid fixation of endometrial brush cytology ensures a well-preserved,representative cell sample with frequent tissue correlation

Endometrium was obtained by brushing 656 hysterectomy specimens with a MedScand cytobrush (Hollywood, FL) in order to evaluate linking an easy-to-use liquid fixative with brush-sampling of the endometrium, and to determine the fixative's operating characteristics when applied to brush-sampling the endometrium. Liquid-fixed brush-samples yielded 4 to 6 slides per case, and any one slide accurately represented the case diagnosis. Tissue fragments were incidentally collected by the brush in 524 cases (79.9%), were least often obtained from senile and weakly proliferative endometria, and were always obtained from high-grade endometrial hyperplasias and carcinomas. With tissue fragments obtained by brush-sampling, the diagnosis matched that of the hysterectomy endometrium. Cytology alone separated benign endometrium from high-grade atypical hyperplasia and carcinoma, showing that clinical decision-making can be based on endometrial brush cytology collected into a liquid fixative. Furthermore, the addition of tissue-fragment histology increased the diagnostic accuracy of the endometrial brushing procedure to 92.5% overall and 100% for high-grade atypical hyperplasias and carcinomas. At this juncture, we advocate endometrial brushing as a sensitive and generally specific case-finding technique; and perhaps as experience teaches us to distinguish low-grade nontypical hyperplasias from purely physiological changes of the endometrium, it may become accepted as a definitive diagnostic method. The advantages of examining a liquid-fixed suspension are that 1) because these preparations are homogeneous from slide to slide, with any one slide affording the same diagnosis as any other slide from the same case, the number of slides examined may be greatly reduced, and 2) tissue fragments can be used to quality-control the cytology diagnosis in the majority of cases, especially in cases of endometrial hyperplasia and carcinoma. Diagn Cytopathol 1996;14:367–373. © 1996 Wiley-Liss, Inc.     

置铜宫内节育器前后子宫内膜微血管密度及VEGF检测

目的:检测置铜宫内节育器(Cu-IUD)前后妇女子宫内膜组织中微血管密度(MVD)及血管内皮生长因子(VEGF)的变化.方法:免疫组化法检测10例放置Cu-IUD妇女置器前后子宫内膜VEGF的表达.以CD34标记血管内皮细胞,计数置器前后妇女子宫内膜MVD.结果:置器后子宫内膜VEGF的表达及MVD均高于置器前(P＜0.05),且2者的表达呈正相关(r=0.847,P＜0.05).结论:Cu-IUD可能通过促进妇女子宫内膜VEGF的表达,引起子宫内膜微血管结构和功能的改变,参与置器后异常出血的发生.