全文获取类型
收费全文 | 48834篇 |
免费 | 3669篇 |
国内免费 | 2468篇 |
专业分类
耳鼻咽喉 | 254篇 |
儿科学 | 1361篇 |
妇产科学 | 471篇 |
基础医学 | 11262篇 |
口腔科学 | 387篇 |
临床医学 | 3679篇 |
内科学 | 13570篇 |
皮肤病学 | 578篇 |
神经病学 | 1215篇 |
特种医学 | 621篇 |
外国民族医学 | 7篇 |
外科学 | 2107篇 |
综合类 | 6324篇 |
现状与发展 | 18篇 |
预防医学 | 7336篇 |
眼科学 | 386篇 |
药学 | 2469篇 |
13篇 | |
中国医学 | 852篇 |
肿瘤学 | 2061篇 |
出版年
2024年 | 47篇 |
2023年 | 607篇 |
2022年 | 1861篇 |
2021年 | 2146篇 |
2020年 | 1693篇 |
2019年 | 1477篇 |
2018年 | 1493篇 |
2017年 | 1466篇 |
2016年 | 1731篇 |
2015年 | 2033篇 |
2014年 | 2822篇 |
2013年 | 2991篇 |
2012年 | 2756篇 |
2011年 | 3237篇 |
2010年 | 2684篇 |
2009年 | 2445篇 |
2008年 | 2397篇 |
2007年 | 2532篇 |
2006年 | 2274篇 |
2005年 | 2002篇 |
2004年 | 1775篇 |
2003年 | 1547篇 |
2002年 | 1240篇 |
2001年 | 1204篇 |
2000年 | 989篇 |
1999年 | 880篇 |
1998年 | 755篇 |
1997年 | 770篇 |
1996年 | 627篇 |
1995年 | 631篇 |
1994年 | 594篇 |
1993年 | 473篇 |
1992年 | 354篇 |
1991年 | 302篇 |
1990年 | 269篇 |
1989年 | 244篇 |
1988年 | 231篇 |
1987年 | 167篇 |
1986年 | 142篇 |
1985年 | 287篇 |
1984年 | 180篇 |
1983年 | 134篇 |
1982年 | 120篇 |
1981年 | 98篇 |
1980年 | 68篇 |
1979年 | 58篇 |
1978年 | 51篇 |
1977年 | 31篇 |
1976年 | 31篇 |
1975年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Sundaram Hariharan Eric P. Cohen Brahm Vasudev Rimas Orentas Raphael P. Viscidi Justin Kakela Brian DuChateau 《American journal of transplantation》2005,5(11):2719-2724
We evaluated twenty renal transplant subjects at various stages of BKV nephritis (BKVN) for BKV-specific IgG and IgM antibodies using ELISA technique and BKV-DNA using PCR. They were divided as early onset (n = 7), stabilizing (n = 3), resolved (n = 8) and late onset (n = 2) BKVN. BKV-specific antibodies and BKV-DNA were simultaneously determined. The mean BKV-specific IgG level in early onset and stabilizing BKVN were 64 and 39 EIA units, and were significantly lower than 138 EIA units seen in resolved BKVN, P = 0.007, P = 0.008. The mean BKV-specific IgM levels in stabilizing BKVN was higher than resolved BKVN (130 vs 51 EIA units), P = 0.006. Mean plasma BKV loads for each group were 955,925, 5642 and 42 copies/mL of plasma, respectively. Prospective study in six BKVN cases revealed mean IgG, IgM levels and BKV-DNA at the time of diagnosis of BKVN as 39, 110 EIA units and 586,758 copies/mL of plasma, respectively. After a mean period of 5.2 months, IgG level increased to 120 EIA units (p = 0.0058) and had no detectable viral copies in circulation. Recovery from BKVN and elimination of BKV is associated with the development of BKV-specific IgG antibodies and this provides insight into the role of humoral immunity to BKV in the pathogenesis of BKVN. 相似文献
82.
Tae-Jin Song M.D. Ph.D. David P. Eisenberg M.D. Prasad S. Adusumilli M.D. Michael Hezel B.S. Yuman Fong M.D. 《Journal of gastrointestinal surgery》2006,10(4):532-542
The rising incidence of hepatocellular carcinoma (HCC) in western countries, along with the poor prognosis offered by present-day
treatment modalities, makes novel therapies for this disease necessary. Oncolytic herpes simplex viruses (HSV) are replication-competent
viruses that are highly effective in the treatment of a wide variety of experimental models of human malignancies. This study
seeks to investigate the effectiveness of oncolytic herpes viruses in the treatment of primary HCC cell lines. Sixteen commercially
available human HCC cell lines were studied. G207 is an attenuated, replication-competent, oncolytic HSV engineered to selectively
replicate within cancer cells. Cell lines were tested for viral sensitivity to G207 and their ability to support viral replication
using standard cytotoxicity and viral replication assays. Eleven of 16 cell lines were moderately to highly sensitive to G207
viral oncolysis. HCC cell lines additionally demonstrated the ability to support viral replication in vitro with as high as
800-fold amplification of the administered viral dose observed. G207 is cytotoxic to, and efficiently replicates within, HCC
cell lines in vitro. From these data, we suggest that oncolytic HSV therapy may have a role in the treatment of HCC, and in
vivo studies are warranted.
Presented in part at the 2005 American Hepato-Pancreato-Biliary Association Congress, Hollywood, Florida, April 14–17, 2005.
Supported by grants R01CA75461 and R01CA72632 from the National Institutes of Health, and by grant MBC-99366 from the American
Cancer Society (Yuman Fong). 相似文献
83.
84.
I. Tsunoda Yuzo Iwasaki Hiroshi Terunuma Kazuya Sako Yoshiro Ohara 《Acta neuropathologica》1996,91(6):595-602
Theiler’s murine encephalomyelitis viruses (TMEV) are divided into two subgroups on the basis of their different biological
activities. The GDVII strain produces acute polioencephalomyelitis in mice, whereas the DA strain produces demyelination with
virus persistence in the spinal cord. A comparative study of GDVII and DA strains suggested that low host immune responses
are responsible for the development of acute GDVII infection and that the persistence of infected macrophages plays a crucial
role in the development of chronic white matter lesions in DA infection. All 78 mice infected with GDVII died or became moribund
by day 13, while none of 54 mice infected with DA died. In the acute stage, the distribution of viral antigens in the central
nervous system (CNS) tissue was similar in both GDVII and DA infections, although the virus titer was higher in GDVII infection.
In DA infection, a substantial number of T cells were recruited to the CNS on day 6 when they were virtually absent in GDVII
infection. The titer of neutralizing antibody was already high on day 6 in DA infection but was negligible in GDVII infection.
Development of chronic paralytic disease from day 35 of the DA infection was accompanied by focal accumulation of viral antigen-positive
macrophages in the spinal white matter. In addition, white matter lesions comparable to those in chronic DA infection were
induced in the spinal cord within 7 days after intracerebral injection of DA-infected murine macrophages.
Received: 26 June 1995 / Revised, accepted: 27 December 1995 相似文献
85.
86.
JIN-TOWN WANG TEH-HONG WANG JAW-TOWN LIN CHA-ZE LEE JIN-CHUAN SHEU DING-SHINN CHEN 《Journal of gastroenterology and hepatology》1995,10(4):454-458
A national screening programme for antibody to hepatitis C virus (HCV) in blood donors in Taiwan began in July 1992 using a second-generation immunoassay. To study the impact of this screening on post-transfusion hepatitis in Taiwan, a prospective study on post-transfusion hepatitis, that was started in 1987, was continued. As of June 1994, 245 patients who received a blood transfusion after July 1992 had completed a follow-up period for more than 6 months post-transfusion. Of them, seven (2.8%) recipients developed acute post-transfusion hepatitis. The hepatitis in six cases could not be attributed to infection by hepatitis A, B, C, D, E viruses or cytomegalovirus (CMV) or Epstein-Barr virus (EBV). The remaining patient seroconverted to both IgG and IgM anti-CMV. All seven patients recovered in 6 months without development of chronicity, and the mean peak alanine aminotransferase level was lower compared with that of the cases before anti-HCV screening (i.e. pre-July 1992). These results indicate that the current anti-HCV screening has effectively interrupted HCV transmission through blood transfusion in Taiwan. 相似文献
87.
Kurt H. Chau George J. Dawson Khalif M. Bile Lars O. Magnius Maria H. Sjogren Isa K. Mushahwar 《Journal of medical virology》1993,40(4):334-338
A newly developed assay for IgA class antibody to hepatitis E virus (IgA anti-HEV) was used to study 145 serum samples collected during an outbreak of an enterically transmitted hepatitis that occurred in 3 villages in the lower Shebeli region of Southern Somalia between January, 1988 and November, 1989. A total of 52.4% of the afflicted patients were found positive for IgA anti-HEV, and 73.1% of these were also positive for IgM. Both antibodies disappeared during the convalescence period. Similar results were also seen in serum obtained from sporadic cases of acute waterborne hepatitis in Pakistan. © 1993 Wiley-Liss, Inc. 相似文献
88.
89.
HBV-M定量检测与HBV-DNA含量水平关系的初步探讨 总被引:2,自引:0,他引:2
目的 研究乙型肝炎病毒标志物定量检测结果与其HBV-DNA含量的临床关系。方法 采用时间分辨免疫定量和荧光定量-PCR技术,对HBV-DNA和HBV-DNA含量进行检测。结果 在HBsAg/HBeAg/HBcAb,HBsAg/HBcAb/HBcAb,HBeAg/IC及单纯HBcAb阳性的四个组别中,HBV-DNA阳性率分别是97.17%、30.56%、100%和25%;在HBsAg,HBeAg,HBeAb,HBcAb定量检测高,低值两组HBV-DNA含量对比中,HBV-DNA阳性率分别为76.92%、58.14%;100%、97.26%;20.00%、11.42%;64.62%、27.63%。结论 HBV-DNV比HBV-M更能及时准确反映乙型肝炎病毒感染者的病程情况。 相似文献
90.
LightCycler实时监测PCR定量分析血清HBV DNA 总被引:2,自引:0,他引:2
目的 检验LightCycler实时监测PCR(real-time detection PCR,RTD-PCR)对血清中HBV DNA定量检测的灵敏性和可重复性,探讨HBV血清标志物与HBV DNA定量的关系。方法 HBV定量按深圳匹基公司乙肝PCR荧光检测试剂盒使用说明,对乙肝标志物已明确的773例血清中HBV DNA定量结果进行统计分析。结果 实时监测PCR对血清中HBV DNA定量检测的灵敏性高,可检测低至1000拷贝/ml血清;可重复性好,批间误差<20%;各种标志物类型的血清HBV DNA含量分布情况表明,HBV血清标志物中HBeAg与HBV DNA含量有明显的关系,一般HBeAg阳性血清HBV DNA含量较高,但也有相当一部分例外。结论 LightCycler实时监测PCR对血清中HBV DNA定量检测灵敏性高可重复性好;仅根据HBV血清标志物往往不能确定乙肝患者HBV DNA复制水平的高低,HBV DNA定量检测具有十分重要的临床意义。 相似文献