促甲状腺激素生物素-链亲和素TRFIA定量检测法的建立

用Eu3＋标记链亲和素（SA）,分别用两株识别人促甲状腺素（human thyroid stimulating hormone,hTSH）的单克隆抗体包被96微孔板并生物素化,建立了高灵敏的hTSH生物素-链亲和素（BAS）时间分辨荧光免疫分析（TRFIA）检测法。本法的灵敏度为0.02mIU/L;批内CV为2.7%~3.5%,批间CV为3.2%~3.8%;平均回收率为100.26%;与电化学发光免疫分析技术（ECLIA）比对,相关系数达0.9544,线性方程为Y=1.3133X-32.297;与ECLIA临床测定值也呈明显相关;正常值范围为0.33~3.09mIU/L。本文建立的hTSH-BAS-TRFIA具有灵敏度高,特异性强,稳定性好,测定范围宽,检测时间短和非放射性等优点,具有良好的应用价值。     

Atypical thyrotropin-secreting pituitary microadenoma revealed by severe osteoporosis in a young man

For 10 years, a young man was followed for a severe osteoporosis associated with a considerable reduction in height and a massive weight loss. The constant increase of signs of tissue impregnation with thyroid hormones and the molar ratios of α-TSH suggested an inappropriate secretion of thyrotropin. Magnetic resonance imaging finally revealed a thyrotropic microadenoma of the pituitary gland. This case gives some new additional information on thyrotropin-induced osteoporosis. To our knowledge such a case has never been reported in the literature.     

上皮钙粘附蛋白在甲状腺乳头状癌中的表达与血清TSH浓度相关性的临床研究

目的研究甲状腺乳头状癌中上皮钙粘附蛋白（E-cadherin）的表达与血清TSH浓度的相关性,探讨E-cadherin在甲状腺乳头状癌中的作用及E-cadherin与血清TSH浓度在甲状腺乳头状癌中的相互作用。方法回顾性分析30例甲状腺乳头状癌患者血清TSH浓度,手术切除标本病理切片免疫组化染色后E-cadherin的表达情况。结果（1）E-cadherin在不同性别、年龄、肿瘤大小的甲状腺乳头状癌患者之间的阳性表达率差异无显著性（P〉0.05）;（2）E-cadherin阳性表达率与血清TSH浓度1组（rs=0.287,P〈0.05）、2组（rs=0.298,P〈0.05）、3组（rs=0.474,P〈0.05）均呈正相关,且E-cadherin的阳性表达率随着血清TSH浓度升高而增加。结论甲状腺乳头状癌组织中E-cadherin的表达阳性率与血清TSH浓度之间存在正相关,血清TSH可增进E-cadherin在甲状腺乳头状癌中的表达。探讨二者在甲状腺乳头状癌中的作用与相互关系,对甲状腺乳头状癌患者的治疗及预后判断有深远的意义。     

Reference intervals for TSH and thyroid hormones are mainly affected by age, body mass index and number of blood leucocytes, but hardly by gender and thyroid autoantibodies during the first decades of life

Objectives

The purpose of our study was to establish reference intervals for thyroid function tests in children and adolescents and to identify factors that may influence the limits of these intervals.

Methods

TSH, FT3, FT4, T3, T4, t-uptake, TPO-antibody (TPO-Ab) and TG-antibody (TG-Ab) levels were determined in blood of 1004 infants, children and adolescents by the Elecsys system (Roche).

Results

A distinct overall age-dependent decrease of analyte levels was found for all parameters investigated. Puberty was accompanied by an increase of TSH, FT3 and T3 levels. Results of T4 and t-uptake were significantly higher in girls compared to boys. The exclusion of children with increased TPO-Ab and TG-Ab had no significant effect on the limits of the reference interval. We found that besides age, BMI-SDS but also white blood cells count and gender played a role in the prediction of analyte variation.

Conclusions

Covariates like BMI-SDS and white blood cell count should be taken into consideration when interpreting TSH and thyroid hormone measurements as well whereas gender and TPO-Ab or TG-Ab play a minor role.     

Repeated administration of a Fusarium mycotoxin butenolide to rats induces hepatic lipid peroxidation and antioxidant defense impairment

Butenolide, a mycotoxin elaborated by several toxigenic Fusarium species, frequently contaminates important agricultural products, and has been considered a potential health risk to humans and animals. However, many toxicology issues including toxicity targets and mechanisms of butenolide remain unclear. Previous study indicated that acute butenolide exposure produced hepatic oxidative toxicity, but its chronic toxicity is still unknown. The present study therefore attempted to reveal the adverse effects of repeated butenolide exposure from a viewpoint of oxidative damage focusing on the liver. Intragastic administration of rats with butenolide for seven consecutive weeks resulted in hepatic injury as shown by obvious changes of serum biochemistry parameters indicating liver function. Repeated butenolide exposure also induced oxidative stress as manifested by impairment of antioxidant defenses including depletion of sulfhydryl groups and reduction of glutathione peroxidase activity, and enhancement of lipid peroxidation both in serum and liver. In conclusion, the present study indicated that repeated butenolide exposure induced a significant liver injury, and oxidative damage may serve as a mediator in the toxicity of butenolide. The current findings contribute to the understanding of the toxic profile of butenolide.     

切除甲状腺后胎羊垂体前叶细胞的免疫组化观察

目的 观察切除甲状腺以后胎羊脑垂体中促甲状腺激素（TSH）、促生长激素（GH）和催乳素（LTH）细胞的变化。方法 实验用怀有双胎的母绵羊2只,分别在妊娠中期对其中1只胎羊行甲状腺切除术,另1只胎羊作为正常对照。术后绵羊继续妊娠,待足月后行剖腹产取胎羊。然后用免疫复合物染色（PAP）方法观察胎羊脑垂体细胞。结果 发现去甲状腺胎羊（去甲胎羊）垂体前叶出现肥大的去甲状腺细胞（去甲细胞）,并有两种类型,有的去甲细胞胞浆染色浅,胞浆内有空泡,有的去甲细胞胞浆染色深,无空泡。而在正常对照的胎羊则不出现上述类型细胞。GH细胞大多数较正常组的GH细胞直径小。结论 切除甲状腺以后的胎羊其脑垂体前叶细胞的形态有一定的改变。