ANALYSIS OF SERUM LIPIDS IN PSORIASIS

Serum lipids were determined in 100 psoriatics and the results indicated (1) the incidences of hypercholesterolemia (9%) and hypertriglyceridemia (17%) in psoriatics were significantly higher than in controls; (2) the mean values of serum TC, LDL-C and TC/HDL-C in psoriatics were significantly higher as compared with normal healthy controls matched for sex and age; (3) serum TC, LDL-C and TG values were significantly higher in cases whose disease was progressive or whose lesions involved more than 20% of body surface area than in cases whose disease stationary or lesions less than 20%. As the incidences of hypertension and coronary heart disease were also significantly higher in psoriatics than in controls, the authors proposed that some integrated relationship would exist.     

The “New” Drug-Free Therapeutic Community: Challenging Encounter of Classic and Open Therapeutic Communities

In view of a rapidly changing society, reflected in many changes within the drug-free Therapeutic Communities (TCs), the question has been raised: “What can and cannot be changed in this modality?” This question was addressed at a European conference of experienced therapeutic community workers, who concluded that many changes have occurred and will continue to occur, but some basic concepts should be preserved. The changes inherent in postmodern society are examined here in an effort to foresee their impact on the evolution of the TC. Necessary changes and additions are discussed.     

3,5-di-t-butylcatechol (DTCAT) as an activator of rat skeletal muscle ryanodine receptor Ca2+ channel (RyRC)

In the present study, the effects of 3,5-di-t-butylcatechol (DTCAT) on ryanodine receptor Ca(2+) channel (RyRC) of skeletal muscle sarcoplasmic reticulum (SR) vesicles were investigated, both by monitoring extravesicular Ca(2+) concentration directly with the Ca(2+) indicator dye arsenazo III and by studying the high-affinity [(3)H]ryanodine binding. DTCAT stimulated Ca(2+) release from junctional (terminal cisternae) vesicles in a concentration-dependent manner, with a threshold activating concentration of 30 microM and a pEC(50) value of 3.43+/-0.03 M. The release of Ca(2+) induced by DTCAT was antagonized in a concentration-dependent manner by ruthenium red, thus indicating that RyRC is involved in the mechanism of stimulation. A structure-activity relationship analysis carried out on a limited number of compounds suggested that both hydroxy and t-butyl groups in DTCAT were important for the activation of RyRC. DTCAT inhibited [(3)H]ryanodine binding to SR vesicles with a K(i) of 232.5 microM, thus indicating that it acted directly at the skeletal muscle ryanodine receptor binding site to stimulate Ca(2+) release. In conclusion, the ability of DTCAT to release Ca(2+) from TC vesicles of skeletal muscle is noteworthy in view of its possible use as an alternative compound to either caffeine or halothane for performing the "In vitro contracture test" to diagnose the susceptibility of some patients to develop malignant hyperthermia under particular pharmacological treatments.     

花粉Ⅰ、Ⅱ号及复方花粉片对大鼠实验性高脂血症的影响

借助大白鼠实验性高胆固醇血症模型,以传统降血脂药物烟酸肌醇为对照,观察了花粉Ⅰ,Ⅱ号及复方花粉片对实验性高胆固醇血症的影响。结果表明,复方花粉片能抑制大鼠实验性高胆固醇血症。     

Premovement slow cortical potentials on self-paced hand movements and thalamocortical and corticocortical responses in the monkey

Chronically implanted electrodes on the surface and in the depth of the motor cortex in the monkey could record slowly increasing, surface negative-depth positive potentials that precede self-paced hand movements. Such premovement slow cortical potentials were interpreted to be composed mainly of currents due to excitatory postsynaptic potentials in superficial parts of the apical dendrites of pyramidal neurons through certain thalamocortical projections. To test the interpretation, the same electrodes were utilized to record cerebellothalamocortical responses and corticocortical responses evoked by electrical stimulation of the cerebellar nucleus and of the cerebral cortex. These results were compared with the laminar field potentials of cerebellothalamocortical and corticocortical responses recorded with glass microelectrodes in acute experiments with monkeys. The present study revealed a similarity of the cortical depth profile of premovement slow cortical potentials to that of the dentatothalamocortical responses, and supported the interpretation.     

Mechanisms of CML hyporesponsiveness in long-term renal allograft recipients

Long-term, HLA disparate, renal allograft recipients were assessed by a dose-dependent CML (cell-mediated lympholysis) assay for evidence of donor-specific CML hyporesponsiveness. The frequency of cytolytic T-cell (CTL) precursors capable of responding to donor alloantigen was also examined by a limiting-dilution assay and application of Poisson distribution statistics. Four recipients were found to have depressed CML responses against donor class I HLA antigens (mean 2-5% specific lysis), whereas significant responses against third party targets were noted (15-60% specific lysis). These data are consistent with an antigen-specific defect in the recipient CTL effector mechanism. To determine whether or not this may be due to a deletion of anti-donor alloreactive cells, a sensitive limiting-dilution assay was developed in conjunction with Poisson distribution statistics to obtain the frequency of both anti-donor and anti-third party CTLp present in recipient PBLs. A simultaneous reduction in the number of alloreactive clones and the presence of an active suppressor population were defined, suggesting that several mechanisms may operate concurrently in long-term renal allograft recipients with well-functioning grafts.     

~（99m）Tc标记卵巢癌单抗的实验研究

用９９ｍＴｃ标记卵巢癌单抗，对标记方法、纯化手段、还原后抗体存放时间与标记率关系、标记物免疫活性以及用该标记物对荷瘤裸鼠放免显象进行探讨，提示９９ｍＴｃ标记卵巢癌单抗的可行性。     

小鼠高脂血症模型建立方法的改进

目的:改进小鼠高脂血症模型的建立方法。方法:对脂肪乳处方及配制方法进行改进,采用脂肪乳灌胃建立小鼠高脂血症模型,用酶比色法测定模型组与正常组血清总胆固醇(TC)、甘油三脂(TG)含量。结果:模型组小鼠死亡率低,与正常组相比较,TC和TG具有显著差异。结论:本造模方法成功率高,切实可行。