��ѧ��΢������Һ�����ǶԶ�ͯѪ��λ������õ�̽��

目的探讨应用普通光学显微镜检查尿红细胞形态和UF-50尿液全自动分析仪2种方法对儿童血尿定位诊断的作用和影响因素。方法研究对象为1977-01—2006-01首都儿科研究所肾内科病房收治的临床出现血尿患儿125例,均经肾活检证实为肾小球源性疾病。应用普通光学显微镜对经肾脏病理证实为肾小球疾病和非肾小球疾病患者的晨尿进行红细胞形态的检查;同时应用UF-50尿液全自动分析仪对其中部分肾小球和非肾小球疾病患者的同一标本进行尿红细胞参数测定。结果(1)肾小球疾病组患者均经病理检查所证实。(2)以RBC严重变形≥30%或变形≥70%两者之一作为肾性血尿的诊断标准,其特异度均达到90%以上,但全自动尿液分析仪的灵敏度较低。(3)晨尿和随意尿标本经上述2种方法检查,其肾性血尿阳性率差异无显著性。结论资料显示以RBC变形≥70%或严重变形≥30%作为诊断肾性血尿的标准,其灵敏度和特异度较高;光镜检测尿红细胞形态与UF-50全自动尿液分析仪用于肾小球性血尿检测,其特异度均达到90%以上,但UF-50全自动尿液分析仪的灵敏度较低;儿童可选用随意尿作为2种方法的检测标本。     

光学显微镜和尿液分析仪对儿童血尿定位诊断作用的探讨

目的探讨应用普通光学显微镜检查尿红细胞形态和UF 50尿液全自动分析仪2种方法对儿童血尿定位诊断的作用和影响因素。 方法研究对象为1977 01—2006 01首都儿科研究所肾内科病房收治的临床出现血尿患儿125例，均经肾活检证实为肾小球源性疾病。应用普通光学显微镜对经肾脏病理证实为肾小球疾病和非肾小球疾病患者的晨尿进行红细胞形态的检查；同时应用UF 50尿液全自动分析仪对其中部分肾小球和非肾小球疾病患者的同一标本进行尿红细胞参数测定。 结果(1)肾小球疾病组患者均经病理检查所证实。(2)以RBC严重变形≥30%或变形≥70%两者之一作为肾性血尿的诊断标准,其特异度均达到90%以上，但全自动尿液分析仪的灵敏度较低。(3)晨尿和随意尿标本经上述2种方法检查，其肾性血尿阳性率差异无显著性。 结论资料显示以RBC变形≥70%或严重变形≥30%作为诊断肾性血尿的标准，其灵敏度和特异度较高；光镜检测尿红细胞形态与UF 50全自动尿液分析仪用于肾小球性血尿检测，其特异度均达到90%以上，但UF 50全自动尿液分析仪的灵敏度较低；儿童可选用随意尿作为2种方法的检测标本。     

Assessment of an immature platelet fraction (IPF) in peripheral thrombocytopenia

A new automated method to reliably quantify reticulated platelets, expressed as the immature platelet fraction (IPF), has been developed utilizing the XE-2100 blood cell counter with upgraded software (Sysmex, Kobe, Japan). The IPF is identified by flow cytometry techniques and the use of a nucleic acid specific dye in the reticulocyte/optical platelet channel. The clinical utility of this parameter was established in the laboratory diagnosis of thrombocytopenia due to increased peripheral platelet destruction, particularly autoimmune thrombocytopenic purpura (AITP) and thrombotic thrombocytopenic purpura (TTP). Reproducibility and stability results over 48 h were good. An IPF reference range in healthy individuals was established as 1.1-6.1%, with a mean of 3.4%. Patients in whom platelet destruction might be abnormal, were studied and two of these patients followed serially during the course of treatment. The IPF was raised in several disease states. The most significant increases in IPF values were found in patients with AITP (mean 22.3%, range 9.2-33.1%) and acute TTP (mean 17.2%, range 11.2-30.9%). Following patients during treatment demonstrated that as the platelet count recovered the IPF% fell. These results show that a rapid, inexpensive automated method for measuring the IPF% is feasible and should become a standard parameter in evaluating the thrombocytopenic patient.     

Determination of peripheral blood stem cells by the Sysmex SE‐9500

The Sysmex SE‐9500 automated haematology analyser provides an estimate of immature cells, referred to as ‘haematopoietic progenitor cells’ (HPC). The aim of this study was to evaluate the reliability and usefulness of the SE‐9500 HPC parameter as compared with the CD34 + cell count and to determine whether the HPC count was of value in predicting the optimal harvesting time for peripheral blood stem cells (PBSC). Studies were performed on 112 samples from 21 patients with haematological malignancies and 13 healthy donors undergoing progenitor cell mobilisation. Coefficients of variation for the HPC count were 30%, 23.8%, 12.4% and 8.3% respectively for samples with low (4 × 10⁶/l), medium (13 × 10⁶/l), high (250 × 10⁶/l) and very high (2413 × 10⁶/l) counts. There was good linearity for HPC measurement in both peripheral blood (PB) and purified CD34 + cell suspensions (r > 0.995), and no detectable carryover was observed. There was an acceptable correlation between HPC and CD34 + cell counts for PB samples (r=0.669) and for CD34 + cell suspensions (r=0.859). Analysis of purified CD34 + cells using the SE‐9500 HPC mode revealed that they appear both in the blast cell area and the immature granulocyte area of the analyser cell display. Quantitation of CD34 + cells and HPC during PBSC mobilisation showed good agreement between these parameters with regard to the optimal time for PBSC harvesting. These findings suggest that HPC counting with the Sysmex SE‐9500 may be clinically useful for optimising the timing of PBSC collection.     

Sysmex CS-5100与CA-7000全自动血凝仪抗脂血和溶血干扰能力的初步比较

目的比较Sysmex CS-5100和CA-7000全自动血凝仪抗脂血和溶血干扰的能力。方法以两血凝仪分别测定双水平质控品PT、APTT、Fbg、TT的批内和天间不精密度(CV%)。混合血浆中分别加入终浓度(0~5.0%)甘油三酯(TG)脂肪乳剂和0~32g/L血红蛋白(Hb)溶液,以两台血凝仪测定PT、APTT、Fbg、TT,计算相对偏差(%),偏差以5.0%(PT和APTT)和6.67%(TT和Fbg)为标准。结果 CS-5100和CA-7000 4项测定的批内CV%为0.77%~1.31%,天间CV%为2.00%~3.31%。TG 0~5.0%范围内,两仪器的PT偏差均<5.0%;CA-7000在Hb 16g/L时PT偏差>5.0%(6.32%),CS-5100在Hb(0~32)g/L范围内PT偏差均<5.0%。CA-7000和CS-5100分别在TG 1.0%和2.0%、Hb 1g/L和2g/L时APTT偏差>5.0%(6.90%和7.66%、-8.74%和-7.69%)。CA-7000和CS-5100分别在TG 1.0%和3.0%、Hb1g/L时TT偏差>6.67%(7.37%和7.25%、8.38%和6.45%)。CA-7000在TG 3.0%时Fbg偏差>6.67%(-7.89%),CS-5100在TG 0~5.0%、两仪器在Hb 0~32g/L时Fbg偏差均<6.67%。各浓度TG和Hb时CA-7000的APTT、TT和Fbg测定偏差均大于CS-5100。结论Sysmex CS-5100抗脂血和溶血干扰的能力比CA-7000更强。     

全自动血液分析仪Sysmex XE-2100与Abbott CD-3700的比较分析

目的 比较分析Sysmex XE-2100和Abbott CD-37002类全自动血液分析仪各项检测参数结果的精确度和相关性。方法 选取EDTA-K2抗凝静脉全血,对2台仪器进行精密度和相关性的检测。结果 精密度测定,Sysmex XE-2100血细胞计数各参数变异系数（CV）值位于0．24％～3．56％之间,白细胞分类各参数凹值位于0．66％～37．04％之间;Abbott CD-3700血细胞计数各参数CV值位于0．30％～4．37％之间,白细胞分类各参数CV值位于0．92％～27．91％之间。2台仪器间血细胞计数各参数相关系数的平方（r^2）位于0．9312～0．9987之间。结论 2台仪器各参数精密度符合设计范围,两者之间相关性较好。     

Influence of transport and time on blood variables commonly measured for the athlete biological passport

Some recent studies have characterized the stability of blood variables commonly measured for the Athlete Biological Passport. The aim of this study was to characterize the impact of different shipments conditions and the quality of the results returned by the haematological analyzer. Twenty‐two healthy male subjects provided five EDTA tubes each. Four shipment conditions (24, 36, 48, 72 h) under refrigerated conditions were tested and compared to a set of samples left in the laboratory also under refrigerated conditions (group control). All measurements were conducted using two Sysmex XT‐2000i analyzers. Haemoglobin concentration, reticulocytes percentage, and OFF‐score numerical data were the same for samples analyzed just after collection and after a shipment under refrigerated conditions up to 72 h. Detailed information reported especially by the differential (DIFF) channel scatterplot of the Sysmex XT‐2000i indicated that there were signs of blood deterioration, but were not of relevance for the variables used in the Athlete Biological Passport. As long as the cold chain is guaranteed, the time delay between the collection and the analyses of blood variables can be extended. Copyright© 2015 John Wiley & Sons, Ltd.     

静脉血样品放置时间对Sysmex XE-2100全自动血细胞分析仪测定结果的影响

目的：探讨静脉血样品放置时间对SysmexXE．2100全自动血细胞分析仪测定结果的影响。方法：对38例静脉血标本分别于0、1、2、4、8、24、48h用Sysmex XE-2100进行测定，并将测定结果进行统计比较。结果：大部分参数在8h内没有显著性改变，24h改变明显，而平均血小板体积、血小板分布宽度、大血小板比率和血小板压积在2h内已明显增高。结论：静脉血标本应于8h内用Sysmex XE-2100测定完成。