淡豆豉与黑豆提取物抗癌细胞增殖作用及4种异黄酮成分的含量测定

目的:探讨淡豆豉和黑豆提取物对乳腺癌MCF-7细胞增殖的调节作用,并检测提取物中4种异黄酮成分的含量。方法:MTT法观察淡豆豉和黑豆提取物对乳腺癌细胞株MCF-7细胞增殖率的影响,Annexin/PI法流式细胞仪分析检测细胞的早期凋亡,高效液相色谱法检测淡豆豉和黑豆提取物中4种异黄酮成分大豆苷、染料木苷、大豆苷元和染料木素的含量变化。结果:淡豆豉和黑豆提取物对MCF-7细胞的增殖均有抑制作用且诱发癌细胞凋亡,但高浓度淡豆豉提取物的抑制癌细胞增殖作用明显高于黑豆(P0.05)。淡豆豉提取物中大豆苷、大豆苷元和染料木素的含量显著高于黑豆提取物(P0.01),但染料木苷的含量未见明显变化。结论:淡豆豉提取物与黑豆相比具有较强的抑制癌细胞增殖作用,淡豆豉中含量较高的异黄酮成分大豆苷、大豆苷元和染料木素,可能是其较强的抗癌细胞增殖作用的物质基础。     

大豆卵磷脂对大鼠调节血脂作用的实验研究

目的研究大豆卵磷脂对大鼠调节血脂的作用。方法将雄性SD大白鼠根据TC水平随机分为空白对照组、高脂模型对照组和大豆卵磷脂低、中、高3个剂量组,剂量组给予高脂饲料和不同剂量的受试物。45 d后取血,测血清TC、TG、HDL-C三项指标。结果0.67 g/kg BW、2.00 g/kg BW剂量组的大豆卵磷脂能使大鼠血清中TC、TG降低,与高脂模型对照组比较差异有统计学意义(P<0.05)。结论大豆卵磷脂对大鼠具有降血脂作用。     

大豆蛋白与动物蛋白对糖尿病肾病患者肾功能影响的Meta分析

目的通过Meta分析评价大豆蛋白与动物蛋白对糖尿病肾病患者肾功能的不同影响,为临床实践提供参考。方法收集1980年1月—2011年11月间公开发表的关于糖尿病肾病摄取大豆蛋白和动物蛋白的研究文献,应用RevMan5．0录入相应资料,对相关肾脏指标进行Meta分析。结果共有5篇文献纳入研究,对相关指标进行异质性检验,根据结果分别采用随机效应模型或固定效应模型,Meta分析结果显示：大豆蛋白与动物蛋白相比,可以改善内生肌酐清除率[WMD=-11．51,95％ci（-16．37,6．65）,P〈0．05]、蛋白尿[WMD=-0．96,95％c1（-1．36,-0．58）,P〈0．05]、尿肌酐[WMD=-1．11,95％CI（-1．91,-0．30）,P〈0．05],而对肾小球滤过率[WMD=0．28,95％CI（-0．12,0．68）,P〉0．05]、血清尿素氮[WMD=-0．20,95％CI（-0．60,0．19）,P〉0．05]、血清肌酐[WMD=-0．22,95％CI（-0．54,0．10）,P〉0．05]的影响差异无统计学意义。结论大豆蛋白与动物蛋白相比,在改善肾脏尿液内生肌酐清除率、尿肌酐、蛋白尿等方面具有一定优势,而在肾小球滤过率方面逊于动物蛋白。     

围绝经期综合征中西医临床研究进展

本综述回顾了围绝经期综合征的中医西医病因病机、治疗现状,并展望了该病的可能研究方向。特殊的身体时期下丘脑一垂体一卵巢这个内分泌轴发生变化,导致一系列植物神经紊乱为主的症状。中医认为本病肾虚为本,同时肝气郁结、痰瘀内生等密切相关。西医治疗以激素替代疗法为主,大豆异黄酮治疗以及心理治疗也取得一定的研究进展。中医以辨证治疗为主,专方专药、针灸疗法也广泛应用于临床。中医治疗可能有一定的治疗效果,但是低质量的随机对照研究充斥,尚无充分的循证医学证据肯定其疗效。严格的中药治疗围绝经期综合征的随机双盲对照试验是未来的研究重点。     

Effects of parenteral soybean oil lipid emulsion on the long-chain polyunsaturated fatty acid profile in very-low-birth-weight infants

Aim: Conventional soybean lipid emulsions contain no docosahexaenoic acid (DHA) or arachidonic acid (AA). We investigated the relationship between blood DHA and AA status in 27 very‐low‐birth‐weight (VLBW) infants with or without parenteral lipid emulsion. Methods: Sixteen infants received parenteral lipid emulsion, and 11 infants were control group. The fatty acid composition of the erythrocyte membrane was analysed at birth and at 2 weeks of age. Results: No significant difference in AA levels was observed in the lipid emulsion group between the two time points, whereas the AA levels at 2 weeks were significantly lower than at birth in the control group. The DHA levels in both groups at 2 weeks were significantly lower than at birth, but no group differences were observed at both time points. Conclusion: The use of parenteral soybean oil lipid emulsions in VLBW infants in the postnatal period may prevent the decline in the AA level but does not appear to influence the DHA level.     

Synergistic Effects of High-dose Soybean Intake with Iodine Deficiency, but Not Sulfadimethoxine or Phenobarbital, on Rat Thyroid Proliferation

The specificity and dose dependence of the synergistic effects of soybean intake with iodine deficiency on the induction of thyroid proliferation were investigated in female F344 rats. In the first experiment, rats were divided into 6 groups, each consisting of 5 annuals, and fed a basal diet containing 20% gluten, an iodine-deficient basal diet alone or an iodine-deficient diet containing 0.2%, 1.0%, 5.0% or 25% defatted soybean for 5 weeks. Soybean feeding synergistically induced thyroid hyperplasias with iodine deficiency only at the 25% dose. In the second experiment, rats were also divided into 6 groups, each consisting of 5 animals, and fed a basal diet, a diet containing 20% defatted soybean, 0.025% Sulfadimethoxine (SDM), 20% defatted soybean+0.025% SDM, 0.05% phenobarbital (PB) or 20% defatted soybean+0.05% PB for 5 weeks. The SDM treatments significantly (P<0.05-0.01) increased the thyroid weights, but this increase rate was less prominent in the SDM+soybean group than in the SDM alone group. The PB treatment was also associated with a tendency for increase in thyroid weight, but again this was smaller in the PB+soybean group than in the PB alone group. Although the SDM or PB treatments reduced the serum triiodothyronine and thyroxine levels and consequently increased the serum thyroid-stimulating hormone (TSH) levels, the soybean feeding did not affect or rather attenuated these changes. Our results clearly indicate that soybean feeding does not synergistically enhance the effects of SDM or PB on the rat thyroid. Thus it can be concluded that soybean intake specifically interacts with iodine deficiency in induction of thyroid proliferative lesions in rats, only at high doses.     

醇变性大豆蛋白的结构特征及物化性质的研究

圆二色性分析结果表明，醇变性使大豆蛋白α－螺旋构象的含量增加，结构更加有序；同时，荧光偏振测定显示大豆蛋白分子的链运动自由度发生变化。另一方面，大豆蛋白紫外差示光谱未出现负峰，荧光发射波长不变，说明蛋白质的疏水核未被破坏，因而是一种局部变性。运用″溶剂吸附″模型对大豆蛋白的醇变性过程作了理论阐述。     

大豆异黄酮对去势大鼠脂质过氧化及肝超微结构的影响

目的 研究大豆异黄酮(soybean isoflavone,SI)对去势大鼠脂质过氧化及肝超微结构的影响.方法 70只雌性SD大鼠按血清总胆固醇水平随机分为7组:高脂、雌激素,低、中、高剂量SI干预、假手术及正常对照组.前5组摘除双侧卵巢,恢复1周后开始干预,实验周期为12周,灌胃给药,每周称重1次,分别在去卵巢、给药4、8周、处死时抽取尾静脉血,实验结束后取肝脏标本,分别测定胆固醇(TC)、甘油三酯(TG)、血清高、低密度脂蛋白胆固醇(HDL-C、LDL-C)、脂蛋白(a)及抗氧化酶活性等.结果 SI干预组LDL-C水平显著低于高脂组但仍高于正常对照组;干预可使脂蛋白(a)水平发生变化但对HDL-C影响不显著;高剂量SI能缓解因去卵巢造成的肝细胞超微结构破坏,维护其完整性,全视野下以高剂量干预组与正常组结构最为接近,高脂组镜像损坏明显.结论 SI对去卵巢大鼠的脂蛋白(a)、脂质过氧化及肝超微结构存在影响,高剂量持续干预能降低去卵巢大鼠的LDL-C,并能维持肝超微结构.     

大豆纤维对实验性高脂大鼠动脉粥样硬化的预防作用和抗氧化效果

研究大豆纤维（ＳＦ）对实验性高脂大鼠的血液和肝组织脂质过氧化物（ＬＰＯ）水平、谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性和血脂指标的影响。结果表明，大豆纤维膳能明显降低血清总胆固醇（ＴＣ）、低密度脂蛋白胆固醇（ＬＤＬ－Ｃ）和致动脉粥样硬化指数（ＡＩ）（Ｐ＜０．０５）。血清和肝组织ＬＰＯ水平明显下降，ＧＳＨ－Ｐｘ活力显著提高（Ｐ＜０．０５）．ＳＦ对血脂降低和对氧化低密度脂蛋白（Ｏｘ－ＬＤＬ）的阻断作用可能对防止动脉粥样硬化（ＡＳ）的形成起主要作用。     

膳食蛋白质对大鼠血浆脂类代谢的影响

分别用含酪蛋白、大豆蛋白及花生蛋白的饲料和等量胆固醇喂养大鼠28天,各组大鼠体重增加无明显差别,酪蛋白组大鼠血清总胆固醇(T-CH)、甘油三脂(TG)及极低密度和低密度脂蛋白-胆固醇(VLDL LDL)-CH/T-CH的百分比较大豆蛋白组和花生蛋白组明显升高(P<0.01),血清卵磷脂胆固醇转脂酰基酶(LCAT)的活性和高密度脂蛋白-胆固醇(HDL-CH)/T-CH的百分比明显低于大豆蛋白组和花生蛋白组(P<0.01)。