Lunasin potentiates the effect of oxaliplatin preventing outgrowth of colon cancer metastasis, binds to α5β1 integrin and suppresses FAK/ERK/NF-κB signaling

The effect of lunasin on colon cancer metastasis was studied using three human colon cancer cell lines in vitro and a liver metastasis model of colon cancer in vivo. Lunasin bound with α₅β₁ integrin and internalized into the nucleus of KM12L4 human colon cancer cells. Lunasin (10 μM) inhibited the activation of focal adhesion kinase (FAK) by 28%, 39% and 60% in RKO, HCT-116 and KM12L4 human colon cancer cells, respectively. Lunasin caused an increase in the expression of the inhibitor of kappa B alpha (IκB-α), a decrease in nuclear p50 NF-κB and a reduction in the migration of cancer cells. Lunasin (4 mg/kg bw) inhibited metastasis and potentiated the effect of oxaliplatin by reducing the expression of proliferating cell nuclear antigen. Liver metastatic nodules were reduced from 28 (PBS) to 14 (lunasin, P = 0.047) while combination of lunasin and oxaliplatin to 5 (P = 0.004). The tumor burden was reduced from 0.13 (PBS) to 0.10 (lunasin, P = 0.039) to 0.04 (lunasin + oxaliplatin, P < 0.0001). Moreover, lunasin potentiated the effect of oxaliplatin in modifying expression of proteins involved in apoptosis and metastasis including Bax, Bcl-2, IKK-α and p-p65. Lunasin inhibited metastasis of human colon cancer cells by direct binding with α₅β₁ integrin suppressing FAK/ERK/NF-κB signaling, and potentiated the effect of oxaliplatin in preventing the outgrowth of metastasis.     

Biological and molecular characterization of Soybean yellow common mosaic virus, a new species in the genus Sobemovirus

A novel soybean-infecting sobemovirus termed Soybean yellow common mosaic virus (SYCMV) was characterized. The virus has a single, positive-strand RNA genome of 4152 nucleotides. The virus contains four putative open reading frames encoding P1 (78-566 nt), polyprotein ORF2a (524-2248 nt), polymerase domain ORF2b (1852-3417 nt), and CP (3227-4030 nt). The entire nucleotide sequence of SYCMV showed 31.2-71.3% nucleotide identity with the previously known eleven species of sobemovirus. In host range analysis of SYCMV, in which twenty one species and three different Nicotiana tabacum cultivars belonging to seven families were inoculated with the virus, SYCMV had a narrow host range, infecting only Glycine max and G. soja. Based on the obtained sequence, full-length clones of SYCMV were constructed. Symptoms produced by inoculation with clones were indistinguishable from those produced by inoculation with sap from symptomatic plants. Viral RNA accumulation of SYCMV was detected in the upper leaves by Northern blotting. This indicated that full-length clones of SYCMV were sufficient to produce disease symptoms. Genomic organization, the predicted amino acid sequence, and phylogenetic analyses with known sobemoviruses confirmed the assignment of SYCMV as a new member of the genus Sobemovirus.     

固体发酵产纳豆激酶工艺条件的研究

本文以大豆为原料,采用固体发酵法制备纳豆激酶,并对其发酵工艺条件进行了研究。实验结果表明,固体发酵产纳豆激酶的最佳发酵条件为:发酵时间48小时,发酵温度30～37℃,培养基初始pH值7.0,培养基的含水量70%。     

饲喂不同水平热处理大豆对肉牛体组织和血清中类胰岛素生长因子-Ⅰ、生长激素、胰岛素和瘦素水平的影响

本试验选用12头24月龄杂交肉牛(利木赞×鲁西黄牛,平均体重为532.6±20.9kg)作为试验牛,研究了不同水平的热处理大豆对肉牛背最长肌、背部脂肪、肝脏和血液中类胰岛素生长因子-Ⅰ和生长激素的影响。试验牛被随机分为三组,每组包括4头动物,试验日粮分别含有3.60%、10.06%和15.72%的粗粉碎热处理大豆,记为处理1、处理2和处理3。试验期为28d,试验结束后采集背最长肌、背部脂肪、肝脏和血液样品,测定类胰岛素生长因子-Ⅰ、瘦素、生长激素和胰岛素水平。试验结果表明,三个处理组肝脏和背部脂肪中的类胰岛素生长因子-Ⅰ水平之间没有显著差异(P>0.05);添加10.06%大豆组的背最长肌的胰岛素样生长因子-Ⅰ水平显著高于其他两个处理组(P<0.05);三个处理组的肝脏和背部脂肪中的瘦素含量没有显著差异(P>0.05)。处理1和处理2背最长肌中的瘦素水平显著高于处理3(P<0.05)。粗脂肪的采食量与肝脏中IGF-I的水平之间存在显著的二次曲线关系。试验结果说明,如果热处理大豆的添加量恰当(在本试验中为10.06%),可以提高背最长肌中的IGF-Ⅰ水平,进而提高肉牛生产性能。试验还发现,粗脂肪采食量是影响肉牛...     

嗜水性气单孢菌利用豆油生产3-羟基丁酸和3-羟基己酸共聚物

以嗜水性气单孢菌Aeromonas hydrophila 4AK4，利用含有豆油的碳源为底物，实现了聚3－羟基丁酸和3－羟基己酸共聚物（PHBHHx)的发酵生产，克服了用月桂酸的生产中碳源成本高、起泡、产物难分离等问题。当以豆油为唯一碳源，经48h培养，通过限制氮、磷、氧3种营养条件，可获得菌体细胞干重（CDW）19.5g/L,PHBHHx质量浓度10.8g/L；若以豆油和月桂酸为混合碳源，获得CDW42.2g/L,PHBHHx质量浓度16.8g/L。发酵进行12h后，3－羟基己酸（3－HHx)在PHBHHx中含量稳定，仅在10％－13％之间变化。结果表明，混合碳源更适合PHBHHx的工业化生产。     

逆转录病毒载体介导人载脂蛋白与卵磷脂胆固醇酰基转移酶基因在骨骼肌的异源性共表达

为探讨人载脂蛋白AI和卵磷脂胆固醇酰基转移酶基因在肌源性细胞中异源共表达的可能性,构建含上述基因和新霉素磷酸转移酶基因的多顺反子重组逆转录病毒载体,以此制备重组病毒颗粒并转染小鼠原代肌母细胞及C2C12肌源性细胞株。酶联免疫吸附法和免疫组织化学检测证实转染后的细胞均具有异源共表达人载脂蛋白AI与卵磷脂胆固醇酰基转移酶的能力,经G418筛选则获得稳定转化的C2C12细胞株,60天后仍能有效共表达人载脂蛋白AI与卵磷脂胆固醇酰基转移酶,聚合酶链反应法检测显示人载脂蛋白AI cDNA与IRES序列均有效整合于靶细胞基因组中,提示以重组逆转录病毒为载体对肌源性细胞进行遗传修饰,再移植回骨骼肌使之在体内长期高效表达载脂蛋白AI和卵磷脂胆固醇酰基转移酶,可能是一种值得探讨的通过促进胆固醇逆转运途径来防止或减轻高脂血症和动脉粥样硬化的方法。