Naturally occuring antibodies against nerve growth factor in human and rabbit sera: comparison between control and herpes simplex virus-infected patients

Antibodies against nerve growth factor (NGF) in sera were detected by enzyme-linked immunosorbent assays (ELISA), by their isolation after passage of sera through NGF immunoadsorbent columns and by their specificity to bind and immunoprecipitate mouse NGF as well as to stain by immunohistochemical methods cellular sites of NGF synthesis. Increased levels of anti-NGF antibodies were found in sera of herpes simplex virus (HSV)-infected patients but not in HSV-inoculated rabbits. As HSV latency is known to be promoted by NGF in vitro, these results may suggest that anti-NGF antibodies modulate the cytokine function of NGF and thus might play a role in HSV infection. The biological function of circulating antibodies against NGF, in general, is now open to future investigation.     

不同分级肝硬化患者血清甲状腺素水平的意义

目的探讨肝硬化患者血清中甲状腺素水平的变化与肝硬化病变程度及其临床实验室检查之间的相关性。方法用放免法测定肝硬化患者血清FT3、FT4、PC-Ⅲ、Ⅳ-C、LN及HA，并按Child—Pugh方法将肝功能分为A、B、C三级。结果随着肝硬化病变程度的加重，血清肝纤维化指标逐渐增高，甲状腺素呈下降趋势，同时血清甲状腺素水平与肝硬化的低蛋白血症、腹水及凝血酶原时间密切相关。结论肝硬化患者体内存在不同程度的甲状腺素水平紊乱，紊乱程度与肝功能受损程度有明显相关性，联合检测血清FT3、FT4、PC-Ⅲ、Ⅳ—C、LN及HA对估计肝硬化病变程度及判断预后有十分重要的临床价值。     

脂膜微泡结合凝血酶原复合物的制备

目的:制备结合凝血酶原复合物(PCC)的阴离子脂膜微泡,评价微泡的理化性质。材料和方法:采用机械振荡直接连接法,分两组(于机械振荡之前或之后加入),制备结合PCC的阴离子脂膜微泡,观察并检测洗涤前后微泡的理化性质。结果:微泡与PCC的结合率及Ⅸ因子活性:两组间比较无明显差异(P>0.05),但洗涤后结合率由洗涤前的95%降为80%,活性由90%降为19%。结论:直接连接法可制备结合PCC的阴离子脂膜微泡,微泡与PCC结合率较高,且洗涤前能保持Ⅸ因子较高的活性。     

中草药醛糖还原酶抑制剂筛选模型的建立

目的：通过观察中药知母对醛糖还原酶活性的抑制作用，建立一种适用于筛选中草药醛糖还原酶抑制剂的模型。方法：大鼠12只随机分成3组：空白对照组、知母组、阳性药物组，分别给予生理盐水、知母水提液、依帕司他水溶液灌胃。采用“通法”给药方案制备含药血清，部分血清经过丙酮预处理。将知母水提液和含药（知母）血清分别加人体外建立的以DL-甘油醛为底物、NADPH为辅酶的醛糖还原酶活性测定体系。结果：丙酮处理过的空白血清较未处理的空白血清对酶活性的影响更小；知母水提液对醛糖还原酶抑制率达99％，含药（知母）血清对醛糖还原酶抑制率达67％。结论：运用血清药理学实验方法建立的筛选模型能有效、可靠的进行中草药醛糖还原酶抑制剂的筛选。     

Lipid peroxidation in several brain regions during development of post-stress depressions in rats with different strategies of adaptive behavior

Lipid peroxidation in the brain cortex, striatum, hippocampus, and hypothalamus of rats of the KLA and KHA lines which were distinguished by their strategies of adaptive behavior was investigated following emotional-painful stress. Significant long-term changes in lipid peroxidation were shown to occur in the brain. These had a phase character and depended on the behavioral characteristics of the animals. The investigated brain regions were characterized by different reactions of lipid peroxidation to the stress. The induced depressive-like states (on the 21st day after the stress) in rats of the KLA and KHA lines were distinguished by the largest changes in lipid peroxidation in the striatum and hypothalamus and in the striatum and hippocampus, respectively. One could conclude on the basis of these results that both identical and different mechanisms of formation and development of depression existed in the animals with different behavioral strategies.     

血清瘦素在男生青春发育期的动态观察

目的 探讨血清瘦素在男生青春期发育中的作用。方法 健康男生170名，调查首次遗精时间，测定血清瘦素、睾酮、促卵泡成熟激素、黄体生成激素水平，采用多元Logistic回归模型分析男生青春期发育的影响因素；同时，连续两年观察，分析血清瘦素在青春期的动态变化。结果 末次研究已遗精组血清瘦素、睾酮、促卵泡成熟激素、黄体生成激素水平均明显高于未遗精组。多因素分析显示血清瘦素、睾酮、促卵泡成熟激素、体重为男生青春期发育的独立影响因素。初次资料分析，遗精前组血清瘦素、睾酮明显高于未遗精组。结论 血清瘦素、睾酮、促卵泡成熟激素、体重为男生青春期发育的独立影响因素，男生遗精前期血清瘦素、睾酮已有明显改变。     

应用抑制消减杂交和cDNA表达谱芯片筛选肝星形细胞持续活化的相关基因

目的：利用抑制消减杂交（suppression subtractive hybridization,SSH）和cDNA表达谱芯片筛选早期活化的肝星状细胞与大鼠正常肝脏组织、轻度肝纤维化组织、重度肝纤维化组织中差异表达的基因.方法：从SSH构建的大鼠轻度和重度肝纤维化中两个差异cDNA文库中挑选1 000条上调显著的基因,与正常大鼠4 136条基因克隆制作成一张芯片,筛选持续期活化的肝星状细胞相关基因.结果：获得与HSC持续活化相关的上调基因633条,下调基因715条,其中血清和糖皮质激素调节蛋白激酶（SGK）在正常大鼠基因克隆和2、8周SSH上调差异基因中均呈上调信号.结论：联合应用SSH和cDNA表达谱芯片是筛选和鉴定不同样本中差异表达基因的快速、经济和有效方法;SGK可能作为多种细胞信号传导通路和细胞磷酸化级联反应的一个功能性交汇点,参与了肝星状细胞的早期活化和信号传导.     

绞股蓝多甙对家兔脂质代谢的影响

绞股蓝多甙１００ｍｇ／ｋｇ·ｄ－１×４ｗｋ灌胃给药，观察结果表明该物具有降低实验性高脂血症家兔的血脂水平及血清过氧化脂质、心肌褐脂质含量的作用。     

车前子对高脂血症大鼠脂质过氧化的影响

目的 :　探讨车前子对高脂血症大鼠脂质过氧化的影响。方法 :　采用大鼠血清及多脏器组织 ,观察车前子对高脂血症大鼠脂质过氧化的影响。结果 :　高脂对照组血清和心肌超氧化物歧化酶 ( SOD)活性显著降低 ,而脂质过氧化物 ( LPO)含量明显升高 ,血清和肝脏的过氧化氢酶( CAT)、谷胱苷肽过氧化物酶 ( GSH-Px)活性明显降低。补充车前子后显著升高血清及心肌组织SOD活性 2 2 %和 2 8% ,以及肝脏 GSH-Px活性 2 2 %。而使血清及心肌的 LPO含量分别下降 3 2 %和 1 7%。结论 :　 1 5 g/kg车前子可提高高脂血症大鼠体内抗氧化能力并免受自由基损伤