柴胡剂对单克隆抗体5—1—6引起蛋白尿的实验研究

静脉一次性注射单克隆抗体5-1-6可引起实验大鼠大量的、一过性的蛋白尿。抗体注射后第1、3、5、8天,柴胡剂治疗组与PBS对照组比较有显著性差异。免疫荧光所见,柴胡剂治疗组中沿肾小球毛细血管壁颗粒样沉积明显减少。口腔灌药柴苓汤对蛋白尿的作用未能得到证实。此实验提示柴胡剂可能通过某种途径改变了大鼠肾小球毛细血管壁的通透性。     

刺五加注射液治疗脑出血的临床评价

30例高血压脑出血患者在常规疗法基础上加用紫外线辐射血液疗法作为对照组,另30例在上述疗法基础上加用刺五加注射液静脉滴注为治疗组。结果治疗组显效率96.7％,总有效率100％,对照组显效率60.0％,总有效率83.3％,治疗组明显优于对照组(P<0.01)。表明刺五加注射液不仅用于缺血性脑梗塞的治疗,而且也可用于脑出血,对出血量在30ml以下的病例,疗效较好。     

制首乌中一种新脂肪酮

目的 :考察制首乌 (RadixPolygoniMultifloriPreparata)化学成分。 方法 :应用硅胶硅胶柱层析对制首乌氯仿萃取部分中化学成分进行分离纯化 ,应用理化常数测定和光谱 (IR ,MS ,H NMR ,¹³C-NMR)分析技术鉴定结构。结果 :分离并鉴定了2个单体化合物 ,分别为 :1,2- 二羟基十九酮-3(1,2 dihydroxynonadecone 3,Ⅰ)、没食子酸 (gallicacid ,Ⅱ)。结论 :Ⅰ为新化合物。     

Cardiovascular actions of Radix Stephaniae Tetrandrae: a comparison with its main component, tetrandrine

A comparison of the cardiovascular actions of the extract of Radix Stephaniae Tetrandrae (RST), the root of a Chinese herb Stephania tetrandra S Moore, in rats with those of tetrandrine (Tet), the best known active component of RST was reviewed. The RST extract inhibits Ca2 influx into the myocyte and reduces protein release during reperfusion with a Ca2 containing solution following perfusion with a Ca2 free solution (Ca2 paradox) , and arrhythmia during reperfusion in the isolated perfused heart. It also reduces the infarct size induced by ischemia/reperfusion in vitro and in vivo. In addition, the RST extract suppresses elevation of arterial blood pressure in DOCA-salt hypertensive rats. It does not further reduce the heart rate and coronary flow significantly during myocardial ischemia. The effects are similar to those of Tet. When compared with the same doses of Tet alone, the RST extract, of which 9 % is Tet, produces equally potent effects on infarction, arrhythmias, coronary flow and heart r     

黄芪水煎工艺优选试验

将黄芪等药物进行水煎提取正交试验,分别以水提取物得量和黄芪甲甙收率作考察指标,数据作方差分析。结果表明,各因素间无显著性差异。     

Suppressive effects of<Emphasis Type="Italic">Platycodon grandiflorum</Emphasis> on the progress of carbon tetrachloride-lnduced hepatic fibrosis

The suppressive effects of Platycodi Radix (Changkil: CK), the root of Platycodon grandiflorum A. DC (Campanulaceae), on the progress of acute carbon tetrachloride (CCl4)-induced hepatic fibrosis were investigated in the rat. CK significantly suppressed CCl4-induced hepatic necrosis and inflammation, as determined by the serum enzymatic activities of alanine and aspartate aminotransferase and serum tumor necrosis factor-alpha levels, in dose-dependent manners. In addition, the increased hepatic fibrosis after acute CCl4 treatment was suppressed by the administration of CK. CK also significantly prevented the elevation of hepatic alpha1 (I) procollagen (type I collagen) mRNA and alpha-smooth muscle actin (alpha-SMA) expressions in the liver of CCl4-intoxicated rats and also suppressed the induction of alpha-SMA and type I collagen in cultured hepatic stellate cells, in dose-dependent manners. These results suggest that the suppressive effects of CK against the progress of acute CCl4-induced hepatic fibrosis possibly involve mechanisms related to its ability to block both hepatic inflammation and the activation of hepatic stellate cells.     

十七种不同产地白头翁的质量考察

目的以白头翁皂苷B4对照品作为指标,考察不同产地白头翁的质量真伪优劣。方法TLC、UV、HPLC方法。结果白头翁皂苷B4含量≥5.0%有九种,≤5.0%有8种,其中伪品4种。结论安徽滁州产地道药材白头翁含量高达8.0%,从而证实了地道药材的名副其实。     

黄芪对低氧性肺动脉高压大鼠肺组织血栓素A_2及肺血管结构的影响

目的:探讨黄芪对低氧性肺动脉高压(HPH)大鼠肺腺泡内肺动脉结构重建及肺组织血栓素(TXA2)的影响。方法:雄性Wistar大鼠21只,随机分为3组,每组7只。对照组:室内空气正常饲养;低氧组:在常压低氧舱内(10±0.5)%饲养;治疗组:缺氧+黄芪(12g/kg)。低氧30天分别用右心导管法测定肺动脉平均压(mPAP),放射免疫法测定肺组织中TXA2含量,计算MA、PMA、NMA比例及肺中、小肌型动脉相对中膜厚度(RMT)与相对中膜面积(RMA)。结果:低氧组mPAP、RVHI、TXA2含量较对照组明显升高(P<0.01或P<0.05),治疗组较低氧组明显减少(P<0.05)。低氧组肺小动脉肌化程度、RMT(%)、RMA(%)较对照组明显增多(P<0.01),治疗组较低氧组明显减少(P<0.01)。结论:慢性低氧可引起大鼠肺血管结构重建,黄芪注射液可降低肺组织TXA2含量,可部分逆转肺血管结构重建,降低肺动脉高压。     

滇产三七固态生物转化产物的初步药理研究

目的:对三七生药生物转化后所提总皂苷进行药理研究,初步判断转化效果。方法:采用断头脑缺血、体内血栓形成、氯仿致心律失常及离体心脏灌流实验。结果:腹腔注射1号-5号样品100、200mg/kg,对断头脑缺血小鼠均有明显保护作用;1号200mg.kg-1.d-1×5d,能明显抑制胶原-肾上腺素诱导小鼠体内血栓形成;除1号100mg/kg外,各组均能明显对抗氯仿诱发小鼠心律失常,3、4号高剂量与5号高剂量组相比室颤发生率有所降低;与生理盐水对照组相比,1-4号保留了扩张冠脉,但对心肌收缩力和心脏前负荷的影响各有差异。结论:转化后样品与对照品在药理作用上发生了一定变化,提示转化后的三七总皂苷化学结构可能有所变化,与初步的化学分析结果相吻合。     

脂清胶囊质量标准的研究

目的建立脂清胶囊(虎杖,山楂,当归等)的质量标准控制方法。方法采用薄层色谱法(TLC)对处方中山楂、当归进行定性鉴别;采用高效液相色谱法(HPLC)对虎杖中大黄素进行含量测定。高效液相色谱条件:Agilent1100,ODSC18柱,流动相:甲醇-0.1%磷酸溶液(80∶20)。结果山楂、当归的薄层色谱图斑点清晰,具有专属性;大黄素在0.306~0.816μg范围内线性关系良好,r=0.999 9,平均回收率为99.22%,RSD为2.4%。结论该方法能准确可靠地进行定性、定量检测,能有效地控制脂清颗粒的质量。