细胞周期蛋白D1基因的表达与喉鳞癌相关性研究

目的：探讨细胞周期蛋白Ｄ１的表达与喉鳞癌（ＳＣＣＬ）的关系。方法：对３５例经病理证实ＳＣＣＬ患者的肿瘤组织及其邻近的正常粘膜,运用反转录多聚酶链反应（ＲＴ－ＰＣＲ）、光密度半定量法,分别检测其ｃｙｃｌｉｎ Ｄ１的表达程度。结果：发现几乎所有的肿瘤标本的表达量要高于正常粘膜（Ｐ〈０．０５）,有淋巴结转移者的表达量要明显高于无淋巴结转移者（Ｐ〈０．０５）;表达量与ＴＭＮ分期也有明显的相关性（Ｐ〈０．０     

Cav1.2和Cav3.1在犬左心室不同部位中表达的比较

目的：研究犬左心室三层心肌L型钙通道（I（Ca-L））、T型钙通道（I（Ca-T））主要亚单位mRNA的表达情况,探讨其复极异质性的可能分子基础及意义．方法：应用RT—PCR半定量分析犬左心室外、中、内三层心肌I（Ca-T）,α亚单位（Cavl．2）、I（Ca-T）主要的亚单位（Cav3,1）mRNA的表达量．结果：Cavl．2 mRNA的表达在左心室中层、内层均明显高于外层（P〈0,05）,但在中层和内层之间无统计学差异（P〉0．05）,Cav3．1mRNA在外层几乎缺如,在中层、内层也只有少量表达且无明显统计学差异（P〉0,05）,结论：Cavl,2,Cav3,1 mRNA在犬左心室三层心肌中表达存在明显的差异,这种差异可能构成左心室三层心肌I（Ca-L）及I（Ca-T）分布差异的分子基础,在折返性心律失常的产生及药物治疗中可能起着重要的作用．     

应用基因芯片筛选人高、低转移肺巨细胞癌细胞株差异表达基因的初步探讨

目的应用基因芯片探讨人高、低转移肺巨细胞癌细胞株95D和95C的基因表达差异,筛选肺癌转移相关基因。方法提取人高转移肺巨细胞癌细胞株95D和人低转移肺巨细胞癌细胞株95C的mRNA并反转录为cDNA,分别用Cy5-dUTP和Cy3-dUTP进行标记后与基因芯片杂交,然后用Scan Array 3000扫描仪及Im aGene3.0软件处理杂交信号得到95D和95C的表达差异基因。对部分有差异表达的基因进行RT-PCR分析鉴定。结果在被检测的人高、低转移肺巨细胞癌细胞株95D和95C中,共有466条基因出现表达差异,其中具有同一GenBank ID号的Doub le基因共108对,包括上调基因47对,下调基因61对。有表达差异的F ln29、RUVBL2、C14orf3等基因RT-PCR结果与基因芯片一致。结论多基因参与了肺癌的转移过程,基因芯片技术是筛选肺癌转移相关基因的有效方法。     

Survivin和PTEN蛋白在肝癌中表达关系探讨

目的:探讨Survivin和PTEN蛋白与肝癌临床病理因素的关系以及两蛋白表达之间的可能关系。方法:免疫组化方法检测51例肝癌组织石蜡切片中Survivin和PTEN蛋白的表达。分析蛋白的表达情况与肝癌临床病理因素的关系。结果:(1)Survivin蛋白在肝癌中阳性表达33例(64.70%),阳性表达率和表达强度与肝癌的病理分级有关。(2)PTEN蛋白在肝癌中阳性表达24例(47.06%),其阳性表达率和表达强度与肝癌的病理分级和癌栓形成有关。(3)在同一组织中,Survivin高表达者,其PTEN蛋白表达较低。结论:肝癌中Survivin激活与上调,PTEN失活、下调或缺失,共同参与调控肝癌分化和转移。     

IP-10和IL-6在口腔扁平苔藓中表达的研究

目的:检测正常口腔黏膜与口腔扁平苔藓(OLP)黏膜的上皮和固有层结缔组织中干扰素诱导蛋白10(IP-10)、细胞因子白细胞介素-6(IL-6)mRNA的表达,探讨其在OLP中的作用。方法:应用荧光实时定量RT-PCR技术检测10例OLP黏膜和6例正常口腔黏膜的上皮和固有层结缔组织中IP-10、IL-6mRNA的表达量。结果:OLP黏膜上皮和固有层结缔组织中IP-10、IL-6mRNA的表达均较正常口腔黏膜明显升高(P<0.05),但IP-10、IL-6mRNA的表达量没有明显差异(P>0.05)。结论:OLP组织中趋化因子IP-10、细胞因子IL-6表达量改变可能与其发生发展有一定关系。     

TLR4、MyD88mRNA在胆脂瘤型中耳炎中的表达

目的建立一种实时荧光RT-PCR方法,定量检测中耳胆脂瘤组织中TLR4、MyD88的表达水平。方法选取中耳胆脂瘤组织25份为对照组(其中13份为炎症组,12份为非炎症组);10份正常外耳道皮肤作为正常组。采用实时荧光RT-PCR与Lightcyeler荧光PCR仪定量检测TLR4、MyD88 mRNA的表达水平;采用2-ΔΔCT法处理实时荧光定量RT-PCR数据。结果炎症组TLR4、MyD88mRNA表达水平较正常外耳道组分别上升了4倍和3倍,且差异有统计学意义(P值均<0.05);炎症组TLR4、MyD88mRNA表达水平较非炎症组分别上升了2倍和1倍,且差异有统计学意义(P值均<0.05)。结论中耳胆脂瘤组织中,尤其是炎症反应明显的胆脂瘤组织,TLR4及其下游信号转导分子的表达量发生改变,提示TLR4-MyD88信号传导途径可能参与了胆脂瘤型中耳炎的发病。     

The re-emergence of pertussis in Tunisia

Objective

The authors had for aim to analyze pertussis epidemiology in Tunisia by studying nasopharyngeal specimens of infants hospitalized in Tunis.

Methods

Between march 2007 and march 2008, clinical nasopharyngeal samples were collected from infants with a suspected diagnosis of whooping cough, pertussoid cough, or pertussis-like syndrome, admitted at the Tunis children's hospital. The laboratory diagnostic criteria were culture isolation of Bordetella species on Bordet-Gengou medium and real-time PCR.

Results

Fifty-nine percent of the 74 investigated children with suspected pertussis were less than two months of age. The diagnosis of pertussis was proved positive by real-time PCR for 41%. Culture was negative in all cases.

Conclusions

Whooping cough is still prevalent in Tunisia despite an important vaccination coverage. Real-time PCR is an invaluable tool for the rapid diagnosis of pertussis, however culture must also be associated.     

Minnelide reduces tumor burden in preclinical models of osteosarcoma

Osteosarcoma is the most common bone cancer in children and adolescents with a 5-year survival rate of about 70%. In this study, we have evaluated the preclinical therapeutic efficacy of the novel synthetic drug, Minnelide, a prodrug of triptolide on osteosarcoma. Triptolide was effective in significantly inducing apoptosis in all osteosarcoma cell lines tested but had no significant effect on the human osteoblast cells. Notably, Minnelide treatment significantly reduced tumor burden and lung metastasis in the orthotopic and lung colonization models. Triptolide/Minnelide effectively downregulated the levels of pro-survival proteins such as heat shock proteins, cMYC, survivin and targets the NF-κB pathway.