补肾中药改善高龄不孕女性卵母细胞氧化应激损伤的研究进展

越来越多的高龄不孕女性选择辅助生殖技术(ART)治疗,但这部分女性卵巢储备下降、窦卵泡数减少,卵母细胞质量下降,优质胚胎少,助孕结局并不理想。卵母细胞质量是影响妊娠成功的关键因素,氧化应激是引起颗粒细胞凋亡和卵母细胞质量下降的主要原因。补肾中药在减轻高龄不孕女性卵母细胞氧化应激损伤、抑制颗粒细胞凋亡、改善卵子质量、提高临床妊娠率等方面发挥了重要作用。补肾中药已广泛应用于体外受精-胚胎移植(IVF-ET)的各个阶段,为高龄不孕女性治疗提供了新的有效路径。     

HCG扳机后血清HCG水平对IVF结局的影响

目的 探讨体外受精-胚胎移植(IVF-ET)过程中人绒毛膜促性腺激素(HCG)扳机后12 h的血清HCG水平对IVF结局的影响.方法 选择2015年3月至2019年10月于我中心行长方案IVF-ET助孕的854例患者.根据血清HCG水平分为5组:A组:HCG≤58.78 U/L(n= 42);B组:58.78 U/L＜...     

乌萸汤改善小鼠卵巢储备功能及卵巢反应性的实验研究

目的通过观察卵巢储备功能下降(diminished ovarian reserve,DOR)小鼠促排卵情况及卵巢储备功能,探讨乌萸汤对DOR小鼠的治疗作用。方法以动情周期异常为DOR造模成功标准,将12只造模成功的24周龄C57BL/6J雌性小鼠随机分为高龄组和治疗组,6周龄C57BL/6J雌性小鼠为青年组。治疗组予乌萸汤灌胃8周,青年组和高龄组予等量蒸馏水灌胃。用药第56天起,小鼠腹腔序贯注射妊马雌酮及绒促性素,取卵进行体外受精实验观察其卵母细胞数、成熟卵数及受精卵数,血清抗苗勒管激素检测,卵巢HE染色观察卵巢内各级卵母细胞数量和质量、免疫荧光染色检测α-tubulin及Ki67表达。结果 (1)治疗组小鼠血清抗苗勒管激素较高龄组有明显好转(P<0.05),且与青年组相比无统计学差异(P>0.05);(2)促排后,高龄组卵母细胞数、成熟率、受精率较青年组明显减少(P<0.05),治疗组与高龄组相比均有所升高(P<0.05);(3)高龄组卵巢略萎缩、髓质组织多,其内各级卵母细胞数量减少形态不佳,闭锁卵泡较多;治疗组髓质有所减少,生长卵泡细胞完整,形态有所好转;(...     

Benzodiazepine modulation of the rat GABAA receptor α4β3γ2L subtype expressed in Xenopus oocytes

The effects of benzodiazepines on GABA_A receptors are dependent largely on the particular α subunit isoform that is present in the receptor pentamer. The inclusion of either the α4 or α6 subunit is generally thought to render the receptor insensitive to classical benzodiazepines. We expressed the rat α4β3γ2L subtype in Xenopus oocytes and observed that both diazepam and flunitrazepam significantly potentiated GABA-gated currents. This potentiation occurred at nanomolar concentrations similar to those seen at the most abundant “diazepam-sensitive” receptor i.e., the α1β2γ2 subtype. In the α4β3γ2L receptor, the effects of diazepam and flunitrazepam were inhibited by nanomolar concentrations of the benzodiazepine site antagonists, Ro15-1788 and ZK93426. The presence of the β3 subunit appears to be important for this modulation since diazepam did not affect GABA responses mediated by recombinant α4β1γ2L or α4β2γ2L receptors. Interestingly, when the α4β3γ2L receptor was expressed in HEK293 cells, diazepam and flunitrazepam displaced the relatively non-selective benzodiazepine site ligand, [³H]Ro15-4513, only at high concentrations (>10 μM) demonstrating a lack of high affinity binding for these classical benzodiazepines. Functional studies of the cell-expressed receptors using whole cell recording techniques showed that neither diazepam nor flunitrazepam potentiated GABA-evoked currents although currents were enhanced by nanomolar concentrations of Ro15-4513. These results suggest that the observed benzodiazepine modulation of the α4β3γ2L subtype depends on the expression system used and may be specific for expression in Xenopus oocytes.     

人卵母细胞玻璃化冷冻技术在辅助生殖中的应用

目的:探讨人卵子玻璃化冷冻的可行性及临床应用价值。方法:回顾性分析2011年8月至2012年7月在取卵日因男方因素行卵子玻璃化冷冻的6例患者,解冻卵子后行卵胞浆内显微注射,记录卵子复苏、受精、卵裂及移植后妊娠情况。结果:6例患者共解冻卵子52枚,复苏卵子31枚,复苏率59.62%。对31枚卵子行ICSI,受精21枚,形成胚胎20枚。1例患者取消移植,5个移植周期共移植胚胎9枚,临床妊娠2例,临床妊娠率40%。结论:卵子的玻璃化冷冻是目前保存女性生育力的有效措施。     

Comparative analysis of perinatal outcome of intracytoplasmic sperm injection and frozen-thawed embryo transfer between donor and autologous oocytes北大核心CSCD

Objective To investigate the obstetric outcomes of intracytoplasmic sperm injection and frozen-thawed embryo transfer (ICSI-FET) between donor and autologous oocytes. Methods A retrospective cohort study was conducted to analyze the clinical data of pregnant patients who underwent ICSI-FET in the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University from June 2016 to January 2020. Totally 73 patients with donor oocytes, and 550 patients who used autologous oocytes in the same period, and the patients were matched at 1∶3 with propensity score matching (PSM), then there were 47 patients in donor group and 131 patients in autologous group. The general conditions and obstetric outcomes were compared among donor group and autologous group. Multivariate logistic regression and linear regression were applied to analyze the factors affecting perinatal complications. Results The bilateral antral follicle count (3.08±4.78) and basal estradiol level [(71.55±45.29) pmol/L] in donor group were significantly lower than those in autologous group [14.95±6.42, (132.84±74.89) pmol/L, all P<0.001]. The birth weight of singleton in donor group [(2 916.48±537.55) g] was lower than that in autologous group [(3 326.67±503.43) g], and there was significant difference (P<0.001). There were no significant differences in premature birth rate [21.28% (10/47) vs. 16.03% (21/131), P=0.416] and incidence of hypertensive disorder complicating pregnancy [12.77% (6/47) vs. 7.63% (10/131), P=0.448] between donor group and autologous group, but both of them had an increasing trend in donor group. Oocyte-donated ICSI-FET reduced the birth weight of singleton (MD=-388.225, 95% CI= -625.914-150.537, P=0.002). Conclusion The perinatal outcome of oocyte-donated ICSI-FET is relatively safe, but the birth weight of singleton is lower than that of self-oocyte ICSI-FET. © 2022 Chinese Journal of Endocrine Surgery. All rights reserved.     

促黄体生成素在卵泡发育及辅助生殖促排卵中的作用

促黄体生成素(luteotropic hormone,LH)对于辅助生殖中诱发排卵或超促排卵具有重要作用,该文就LH在支持卵泡发育中的作用作简单综述.     

Number of oocytes and IVF outcomes: Real-world evidence

Randomized controlled trials (RCTs) are gold standard to study the effect of an intervention and establish causation between the treatment and outcomes. However, RCTs have the disadvantage of being expensive, entailing high resource investments and involving only selected patient populations under experimental settings. Real-world evidence (RWE) from real-world data (RWD) involves a heterogenous patient population in real-world settings. RWE is less expensive and quicker than RCTs; it can provide complimentary evidence if methodological challenges, such as residual confounding and susceptibility to bias, are considered when interpreting the findings. This review examines RWE regarding the association between the number of oocytes following ovarian stimulation and IVF outcomes into shaping current IVF practices.     

Transvaginal combined with transabdominal oocyte retrieval in IVF/ICSI cycle: a case report北大核心CSCD

Objective. To improve the technique of oocyte retrieval in in vitro fertilization/ intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) cycles and reduce the cancellation rate of IVF/ICSI cycle. Methods. The clinical data of a case of IVF/ICSI cycle using transvaginal combined with abdominal oocyte retrieval was reported. Results. The right ovary of one infertile patient who received ICSI due to male factors was not detected repeatedly by transvaginal ultrasound during ovulation induction. The right lower abdominal pain occurred day 5 after using gonadotropin. We considered that the position of the right ovary was high due to pelvic adhesion after previous appendectomy, and was further confirmed by abdominal ultrasound. Two mature oocytes were obtained from the left and right ovaries respectively. Four embryos were obtained after ICSI insemination, and biochemical pregnancy occurred after transplantation. Conclusion. Transabdominal ultrasound-guided oocyte retrieval is relatively safe and effective, and can obtain similar clinical outcomes as conventional transvaginal ultrasound-guided oocyte retrieval. For IVF/ICSI women with difficulty in obtaining oocytes through vagina, transabdominal ultrasound-guided oocyte retrieval can be tried. During IVF/ICSI treatment cycles, it is necessary to improve the quality of medical history inquiry, pre-cycle evaluation, ovulation induction monitoring, oocyte retrieval and embryo transfer. © 2022 Chinese Journal of Psychiatry. All rights reserved.