异噻唑啉酮类衍生物抑菌及防腐效果的研究

目的研究异噻唑啉酮类衍生物(KF-88)的抑菌效果，观察其在洗涤剂中的防腐性能。方法采用营养肉汤稀释法，测定该抑菌剂对金黄色葡萄球菌、大肠埃希菌、白色念珠菌和黑曲霉的最小抑菌浓度(MIC)，并在此基础上进行模拟现场试验。结果KF-88对金黄色葡萄球菌和白色念珠菌的MIC为0．0016‰；对大肠埃希菌和黑曲霉的MIC为0．03‰；KF-88在洗洁剂中浓度为1‰时，可抑制其中的金黄色葡萄球菌生长。结论异噻唑啉酮类衍生物对细菌、霉菌和酵母具有很强的抑菌力。其在洗涤剂工业及其他多种生产领域具有较好的应用前景。     

桂枝茯苓胶囊治疗精索静脉曲张临床观察

目的：观察桂枝茯苓胶囊治疗精索静脉曲张的临床疗效。方法：将143例门诊患者根据静脉曲张程度分为Ⅰ度49例、Ⅱ度62例、Ⅲ度32例3组,给予相同的药物,同等剂量,分别治疗3个疗程,疗程结束后比较疗效。结果：Ⅰ度、Ⅱ度、Ⅲ度在第1个疗程的有效率分别是34.7%、有效率29.03%、12.5%（P=0.08）;在第2个疗程有效率分别是Ⅰ度有效率58%、Ⅱ度有效率51.61%、Ⅲ度有效率21.88%（P〈0.05）;第3个疗程：Ⅰ度有效率74%、Ⅱ度有效率58.05%、Ⅲ度有效率28.22%（P〈0.05）。结论：桂枝茯苓胶囊治疗精索静脉曲张有较好的效果,尤以Ⅰ度和Ⅱ度精索静脉曲张为著。     

乙型肝炎病人污染器械清洗方法及其清洗效果

目的研究多酶洗液对乙型肝炎病毒污染器械的清洗消毒效果。方法采用杰力试纸测试方法和单克隆抗-HBs包被反应板方法 ,对清洗后器械清洗质量进行了检测。结果实验组器械经清洗消毒后残留血迹检测阳性1件,阳性率1.0%;对照组中阳性21件,阳性率21.0%。实验组器械清洗消毒后乙型肝炎病毒残留检测6件阳性,阳性率6.0%,而对照组阳性26件,阳性率为26.0%。2组器械清洗消毒后残留血迹检测和乙型肝炎病毒残留检测的差异均有统计学意义。结论采用多酶浸泡清洗对HBsAg阳性患者使用后的器械可以明显提高清洗质量和消毒效果。     

Acute myeloblastic leukemia with active tyrosine protein kinase

High level of tyrosine protein kinase activity was found in a membrane fraction isolated from an acute myeloblastic leukemia, out of 24 leukemias of different origin investigated. The major substrate for tyrosine phosphorylation in vitro was a 58 kDA protein (p58). The phosphorylation proceeded actively at 0 degrees C and was strongly stimulated by Mn2+ ions. Comparison by partial proteolysis of the p58 with similar phosphoproteins from a T-lymphoma line (KE37) and from lectin stimulated lymphocytes showed high similarity. The possible role of the tyrosine kinase activity in this leukemia is discussed.     

白猫洗洁精杀菌效果的实验观察

试验表明，０．５％白猫洗洁精溶液作用１０分钟，可将悬浮于溶液中的金黄色葡萄球菌与大肠杆菌杀灭９９．９％以上；以其浸泡５分钟，可杀灭餐具表面自然菌８０．３６％．     

亚甲蓝法测定雪糕中掺洗衣粉含量的探讨

用亚甲蓝－分光光度法测定雪糕中掺入洗衣粉的含量，该法平均回收率为９４．８％；标准曲线相关系数为０．９９８；相对标准偏差为３．０５％；最低检出量为２．５μｇ；方法不需特殊仪器，具有准确、稳定、操作简单的特点。     

岛津CL8000全自动生化分析仪酸碱清洗剂的研制与应用

目的 配制岛津CL8000全自动生化分析仪酸、碱清洗剂。方法 根据原装瓶上提供的成分，筛选强碱、有机酸以及在强碱、有机酸的溶液中有较好的渗透、乳化、分散和洗涤性能的非离子表面活性剂；检测研制的酸、碱清洗剂理化稳定性；使用原装和研制清洗剂对比做水空白后1号杯不同波长的毫吸光度(mAbs)变化；做部分检测项目的精密度和准确度试验。结果 理化性稳定，1号杯不同波长的毫吸光度(mAbs)均值在一个标准差内，CV＜2％，均满足要求，用研制的清洗剂作洗液检测A1b、G1u、Cr、ALT的质控液20次，均值在一个标准差内，CV分别为2．88％、3．01％、4．77％和4．83％。结论 研制的酸、碱清洗剂达到实验要求，可以代替原装试剂对反应杯的清洗。     

亚甲蓝简单快速测定阴离子合成洗涤剂的方法

目的：亚甲蓝分光光度法是测定生活饮用水中阴离子合成洗涤剂的国家标准方法之一，但是实验操作中需要反复多次萃取，繁琐耗时，试剂用量大。本文以简化操作步骤，改进实验方法为目的。方法：经过试验，可以直接使用50ml比色管，一次性萃取，虹吸有机相直接进行比色分析。结果：该方法操作简单快速，减少了试剂用量，而且精密度和准确度都能满足实验要求。结论：该方法是可行的，值得推广使用。     

Short-term effects of alcohol-based disinfectant and detergent on skin irritation

The most important risk factor for occupational contact dermatitis in hospital personnel is the exposure to irritants such as water, detergents and alcohol-based solutions. This study was undertaken to evaluate the short-term effects of repeated exposure to an alcohol-based disinfectant, to a detergent and to an alcohol-based disinfectant/detergent alternately. The hardening effect in preirritated skin after a 4-week interval was also evaluated. Detergent, disinfectant and disinfectant/detergent alternately were applied daily every 15 min for 6 h for 2 days to the flexor upper arms and forearms of 15 volunteers. A control area was included. After 4 weeks, a sodium lauryl sulfate patch was applied to each area. Irritant reactions were quantified by visual score, transepidermal water loss (TEWL) and skin colour at baseline, D3, D8, D35 and D37. As evaluated by clinical assessment, detergent caused more redness of the skin than both disinfectant applied alone and disinfectant/detergent alternately at D3 and D8, P < 0.001 and P < 0.001, respectively. An increased irritant response for detergent as compared to disinfectant alone and disinfectant/detergent was confirmed by TEWL and colour evaluations, P = 0.001 and P = 0.001 and P = 0.006 and P = 0.009, respectively. No hardening effect in preirritated skin was found after a 4-week interval. In conclusion, hand disinfection with alcohol-based disinfectant or alternate use of disinfectant/detergent causes less skin irritation than hand disinfection with a detergent. This study evaluated the short-term effects of disinfectant and detergent exposure only, and more long-term studies are necessary before recommendations can be made.