Monoamine oxidase-dependent metabolism of dopamine in the striatum and substantia nigra of

Various factors that might influence the development of the independent secondary focus ("mirror" focus) in the monkey were investigated. Not 1 of 29 monkeys developed independent secondary discharges when we varied: area and length of boundary of the primary epileptogenic agent; ease of passage of agent through pia; nature of chemical agent; mechanical manipulation of homotopic cortex; or site of application of primary agent. The possible reasons for the discrepancy between this negative outcome and our earlier success in obtaining independent foci are discussed.     

The Development of Independent Secondary ("Mirror") Discharges in the Monkey: Failure to Replicate Earlier Findings

Various factors that might influence the development of the independent secondary focus ("mirror" focus) in the monkey were investigated. Not 1 of 29 monkeys developed independent secondary discharges when we varied: area and length of boundary of the primary epileptogenic agent; ease of passage of agent through pia; nature of chemical agent; mechanical manipulation of homotopic cortex; or site of application of primary agent. The possible reasons for the discrepancy between this negative outcome and our earlier success in obtaining independent foci are discussed.     

EEG operant conditioning in a monkey model: II. EEG spectral analysis.

EEG power spectral analysis was studied from 14 (alumina-gel) chronically epileptic, undrugged monkeys during an EEG operant conditioning experiment. The composite profile of the average epileptic monkey shows the majority of power to be below 10 Hz. Because of the large variance in the data, no significant changes in the EEG power spectra could be detected as a function of conditioning. The possible reasons for this large variance are discussed. Hypothesis from previous human "biofeedback" studies would allow the prediction that those frequencies corresponding to the mu and sensory-motor-rhythm should negatively covary with seizure frequency. Data from this study did not support such assertions. The method of using spectral analysis for quantifying changes in the EEG which covary with operant conditioning is evaluated.     

3-氯丙二醇和2、3-氧丙醇合并长期、间歇给药对恒河猴及大鼠毒性作用的观察

本文介绍了3-氯丙二醇和2,3-氧丙醇合并长期间歇用药对猴及大鼠的毒性观察,结果表明,以日剂量5mg/kg 3-氯丙二醇和75mg/kg 2,3-氧丙醇合并应用,可导至大鼠可逆性不育;对猴及大鼠的内脏器官、骨髓细胞增生度及微核数量、末梢血液细胞计数、分类,以及猴的染色体常规检查未见明显影响。表明合并用药有用药量小,给药时间短、起效快、毒性低等优点。     

SERUM-FREE CULTURE OF RHESUS MONKEY EMBRYONIC STEM CELLS

Previous studies have shown that the maintenance and proliferation of undifferentiated rhesus monkey embryonic stem (rES) cells requires medium supplemented with fetal bovine serum (FBS). Due to the uncharacterized composition and variation in serum nature, the present study aimed to replace the serum-containing medium with a serum-free medium in the rES cell culture. The results showed that after the initial 48-h culture in the routinely used serum-containing medium, rES cells can grow and proliferate for a prolonged period in the serum-free medium composed of DMEM supplemented with a cocktail of BSA, IGF-1, TGF- &#102, bFGF, aFGF, estradiol, and progesterone. rES cells cultured in the serum-free medium maintained high level of alkaline phosphatase activity and OCT4 level. There was no indication of differentiation as judged by the marker gene expression of all three embryonic germ layers and trophoblast. In addition, serum-free culture would not affect the passage capacity and differentiation potential of rES cells. This work will facilitate the future study of induced differentiation of rES cells and other applications.     

猕猴B病毒囊膜蛋白gD基因真核细胞表达载体? 的构建及表达

目的 构建猕猴B病毒囊膜蛋白gD的真核表达载体, 并且检测其在293T细胞内的表达情况。 方法 首先通过基因合成手段获得含B病毒gD蛋白的基因片段, 在经由PstⅠ和NotⅠ双酶切后连接到pEGFP-N3载体, 随后将构建的pEGFP-N3-GD重组质粒转染到人胚胎肾上皮细胞系293T细胞。再用Western blot检测所提蛋白其在细胞内的表达情况, 并用激光共聚焦分析其在细胞内的表达定位情况。 结果 成功获得携带gD基因的阳性重组质粒pEGFP-N3-GD, 且pEGFP-N3-GD重组质粒能在293T细胞的表面正常表达。 结论 利用真核表达系统, 既能够在细胞表面产生B病毒gD蛋白的特异性重组抗原, 而且可用于B检测抗原的制备。     

Role of Purkinje cells in the ventral paraflocculus in short-latency ocular following responses

We describe the simple-spike activity of Purkin je cells (P cells) in the ventral paraflocculus (VPFL) of behaving monkeys in association with movements of the visual scene that evoke short-latency ocular following re sponses. One group of P cells discharged maximally for downward motion, and the other for motion toward the side of the recording. The onset of the simple-spike re sponse was measured in relation to the onset of ocular following in 24 P cells. The majority of P cells (79%) led by 1–9 ms. At the site of each recording, electrical stimuli (single negative pulses, 1.5–45 A; 0.2 ms in width) were applied and 60% (18/30) of the sites elicited eye movements in the preferred direction of the P cells. The latency of the single-pulse-evoked response in the ipsilateral eye ranged from 8.6 to 10.9 ms. These data suggest that the P cells in the VPFL play a role in ocular following; some discharge early enough to generate the very earliest eye movements.     

猴B病毒抗体不同检测方法的比对

目的猴B病毒(monkey B virus,BV),也称猴疱疹病毒I型(Cercopithecine herpesvirus 1),是重要的人兽共患病原。根据国家标准,猴B病毒作为抗原检测抗体,但由于生物安全问题,抗原制备受到很大限制,因此使用替代抗原进行抗体血清学检测并进行比对验证。方法应用2种ELISA方法(抗原分别为BV和HVP2)和1种免疫酶法EIA方法(抗原为HSV-1)对本实验室135份送检恒河猴血液样品进行筛查,对阳性及可疑样品再以免疫荧光法IFA和Western blot方法(抗原为HSV-1)以及以HSV-1 g C1纯化糖蛋白为抗原的免疫印迹方法验证。结果HVP2-ELISA、BV-ELISA和HSV-1-EIA阳性检出率分别为32.6%、37.8%和34.8%;3种方法检测结果一致的样品占91.1%(123/135),阳性结果可被IFA和WB确证;可疑样品12份,33.3%(4/12)的样品经验证检验为阳性。结论与BV抗原相比,替代抗原HSV-1的敏感性和特异性较HVP2更为接近;阳性样品及可疑样品的确证检验应使用多种方法,避免漏检。