家具行业苯对作业女工生殖系统的影响

目的了解家具行业苯对作业女工生殖系统影响.方法采用问卷调查、作业场所职业卫生学调查和工人健康体检的方法,对结果进行分析.结果接触组月经异常主要表现为经量过多、月经周期缩短,月经异常率与对照组比较差异有显著性(P＜0.05);已婚女工的妊娠贫血、恶阻等合并症的发生率也显著高于对照组(P＜0.05);神经衰弱综合征、白细胞减少等显著高于对照组(P＜0.05).结论苯作业对女工的生殖系统可能有一定影响,今后应加强这方面的劳动保护和做好工人的职业宣传教育.     

Systemic Autoimmune Diseases in Patients Hospitalized with COVID-19 in Spain: A Nation-Wide Registry Study

We aimed to evaluate the clinical outcome of Systemic Autoimmune Diseases (SADs) patients hospitalized with COVID-19 in Spain, before the introduction of SARS-CoV-2 vaccines. A nationwide, retrospective and observational analysis of the patients admitted during 2020, based on the ICD10 codes in the National Registry of Hospital Discharges, was performed. Among 117,694 patients, only 892 (0.8%) presented any type of SAD before COVID-19-related admission: Sjogren’s Syndrome constituted 25%, Systemic Vasculitides 21%, Systemic Lupus Erythematosus 19%, Sarcoidosis 17%, Systemic Sclerosis 11%, Mixed and Undifferentiated Connective Tissue Disease 4%, Behçet’s Disease 4% and Inflammatory Myopathies 2%. The in-hospital mortality rate was higher in SAD individuals (20% vs. 16%, p < 0.001). After adjustment by baseline conditions, SADs were not associated with a higher mortality risk (OR = 0.93, 95% CI 0.78–1.11). Mortality in the SADs patients was determined by age (OR = 1.05, 95% CI 1.04–1.07), heart failure (OR = 1.67, 95% CI 1.10–2.49), chronic kidney disease (OR = 1.29, 95% CI 1.05–1.59) and liver disease (OR = 1.97, 95% CI 1.13–3.44). In conclusion, the higher COVID-19 mortality rate seen in SADs patients hospitalized in Spain in 2020 was related to the higher burden of comorbidities, secondary to direct organ damage and sequelae of their condition. Whilst further studies should evaluate the impact of baseline immunosuppression on COVID-19 outcomes in this population, efforts should be focused on the optimal management of SAD to minimize the impact of the organ damage that has been shown to determine COVID-19 prognosis.     

混合现实技术与态势感知在心脏大血管外科临床教学中的应用

混合现实技术将患者的影像学检查数据进行三维重建,与真实场景进行有机的结合,模拟真实的手术场景应用于临床教学,结合大数据分析技术,构建态势感知数据模型,对教学互动和病情演绎提供数据能力支撑。本文就混合现实技术与态势感知在心脏大血管外科临床教学中的应用进行探索。该技术的应用可以更新带教手段、弥补传统教学模式的弊端、提升教学...     

混合性结缔组织病患者血清免疫球蛋白含量和补体C3C4的相关性的研究

目的 探讨混合性结缔组织病患者血清免疫球蛋白含量和补体C3C4相关性的关系及其临床意义. 方法用免疫速率比浊法测定68例混合性结缔组织病患者血清免疫球蛋白和补体C3C4含量,并与70名健康体检者进行对比分析.结果 患者组免疫球蛋白I gA、I gG和补体C3C4的含量与对照组相比有明显差异,差异具有统计学意义(P<0.05).患者组补体C3C4的含量具有直线相关性,(P<0.05).结论 混合性结缔组织病患者血清免疫球蛋白I gA、I gG和补体C3C4的含量与病情有关,可用于病情判断和预后评价.     

顶空气相色谱法测定注射用胸腺法新中5种有机溶剂残留量

目的 建立同时测定注射用胸腺法新中乙腈、二氯甲烷、醋酸乙酯、苯和苯甲醚5种有机溶剂残留量的顶空气相色谱法。方法 采用Agilent DB-624（30 m×0.53 mm×3 μm）毛细管色谱柱;火焰离子化检测器;进样口温度200℃;检测器温度250℃;载气为氮气;载气体积流量为2.0 mL·min^-1;分流比为10：1;升温程序：起始温度40℃,保持6 min,以8℃·min^-1的速率升温至90℃,保持2 min,再以20℃·min^-1的速率升温至200℃,保持5 min;采用顶空进样方式,顶空加热箱温度80℃,样品瓶平衡时间30 min。进行系统适用性、检测限（LOD）与定量限（LOQ）、线性关系和范围、加样回收率、精密度、稳定性、耐用性考察。结果 注射用胸腺法新中5种残留溶剂在各自线性浓度范围内与峰面积线性关系良好;平均加样回收率在95.7%~106.3%;精密度、稳定性、耐用性均符合要求。5批胸腺法新中均未检出5种有机溶剂。结论 建立的顶空气相色谱法操作简单、灵敏度和准确度高、重现性和耐用性好,可用于注射用胸腺法新中5种有机溶剂残留量的测定。     

灵芝多糖拮抗环孢素A,氢化可的松及抗肿瘤药的免疫抑制作用

灵芝多糖在50,100和200μg·ml~1时可完全拮抗环孢素A,丝裂霉素C,氟尿嘧啶和阿糖胞苷对小鼠混合淋巴细胞反应(MLR)的轻度抑制(抑制50％以下)作用,部分拮抗氢化考的松对MLR的严重抑制(抑制50％以上)作用。     

栀子提取物中大孔吸附树脂的苯残留量检查

目的测定栀子提取物中大孔吸附树脂残留的苯是否符合限量要求.方法使用气相色谱仪,采用DB-624毛细管色谱柱,以氮气为载气,FID检测器,测定苯.结果栀子提取物中未检出苯,检出限为0.06mg·L-1.结论该方法简便、准确、重复性好,可用于栀子提取物的质量控制.     

HPLC方法研究葛根素混合胶束在Beagle犬体内的药代动力学(英文)

采用高效液相色谱法测定Beagle犬口服葛根素原料药及自制葛根素混合胶束后葛根素的血药浓度, 并计算其药代动力学参数, 比较两者的生物利用度。Beagle犬随机分为两组, 采用单剂量灌胃给予葛根素原料药与葛根素混合胶束, 于不同的时间点取血并用HPLC法测定葛根素的血浆药物浓度。HPLC条件: 色谱柱为DIKMA ODS C18柱 (150 mm×4.6 mm, 5 μm), 保护柱为DIKMA ODS C18柱 (8 mm×4 mm); 流动相甲醇-水 (25:75, v/v); 柱温30 °C; 流速1 mL/min; 检测波长250 nm, 内标物为茶碱。采用WinNonlin 6.1软件求算药代动力学参数。葛根素原料药与葛根素混合胶束在Beagle犬体内的药物动力学参数Tmax分别为61.48和202.91 min, AUC分别为515.96和2796.43 min·μg/mL, CL/F分别为232.58和42.91 mL/min/kg。Beagle犬口服葛根素混合胶束与葛根素原料药的相对生物利用度为542.0%。混合胶束制剂明显提高了葛根素在Beagle犬体内的生物利用度, 且减缓消除速率, 延长达峰时间, 起到了长效、缓效的作用。     

TBL+PAL+翻转课堂混合式教学在本科生《护理心理学》教学中的应用

目的 探讨以团队为基础的学习（team-based learning,TBL）+同伴互助学习（peer assisted leaning,PAL）+翻转课堂混合式教学在护理本科生《护理心理学》课程教学中的应用效果。方法 将2018级4个班级146名护理本科生,整群抽样1~2班为试验组（73人）,3~4班为对照组（73人）。试验组采用TBL+PAL+翻转课堂混合式教学,对照组采用传统教学。课程结束后评价两组学生课程期末考试成绩及案例分析成绩,两组学生自评核心能力得分。采用SPSS 18.0进行t检验。结果 试验组学生的期末考试成绩为（82.30±7.77）分、案例分析考核成绩为（46.13±2.91）分,均高于对照组[（74.97±7.05）分、（40.36±4.25）分];试验组学生的自主学习能力得分[（8.03±0.91）分]、分析问题能力得分得分[（7.99±0.99）分、团队合作能力得分[（7.67±1.09）分]及评判性思维能力得分[（7.03±1.33）分]均高于对照组[（6.21±1.17）、（5.64±1.28）、（5.79±1.27）、（5.15±1.16）]。结论 TBL+PAL+翻转课堂混合式教学将3种教学形式取长补短、优势整合,促进学生对知识点的理解和掌握运用,提高学生自主学习能力、团队合作能力及分析问题能力,强化学生综合知识与技能的迁移及应用能力,提升教学效果,同时也拓展了《护理心理学》的教学思路。     

Ductal prostate cancer: Clinical features and outcomes from a multicenter retrospective analysis and overview of the current literature

ObjectiveThe aim of the study is to evaluate clinical features and outcomes after different therapeutic strategies for ductal prostate adenocarcinoma (DPC), a rare but aggressive subtype of invasive prostate cancer (PCa) accounting for, in the pure and mixed form, 1% or less and 5% or less, respectively, of all the newly diagnosed PCa.Materials and methodsPatients with a proven diagnosis of DPC undergoing surgery, radiotherapy, and androgen deprivation therapy, alone or in combination, were considered for this multicenter, retrospective study. The study assessed overall survival (OS), disease-free survival (DFS), and age-related disease-specific survival.ResultsEighty-one patients met the study inclusion criteria. Pure DPC was found in 29 patients (36%) and mixed ductal-acinar-PCa in 52 patients (64%). After a median follow-up of 63 months (range, 3–206 months), 3- and 5-year OS rates were 84% and 67%, respectively, and 3- and 5-year DFS rates were 54% and 34%, respectively. There were no significant differences in OS or DFS between the pure and mixed DPC groups. Pure DPC was associated with a higher rate of metastatic disease at onset. Patients 74 years or younger had better disease-specific survival (p=0.0019). A subgroup analysis favored radiotherapy as the primary treatment for nonmetastatic, organ-confined DPC (3- and 5-year DFS of 80% and 50%, respectively, compared with 5-year DFS of 35% for surgical patients; p = 0.023).ConclusionsOur study found DPC to be rarer, more aggressive, more likely to metastasize, and have a worse prognosis than the common acinar variant, especially in its pure form. Multicenter series are encouraged to obtain large data sets, or propensity score matching analyses with patients with conventional PCa are desirable to understand the best therapeutic approach and improve outcomes.