Autoradiographic Studies of 3H-estramustine in the Rat Ventral Prostate

Abstract The alkylating agent ³H-estramustine was administered to castrated male rats and found to accumulate in the epithelium of the ventral prostate 5 and 20 min. following intravenous administration, as judged from autoradiography. Four hrs. after intravenous injection of this isotope, the radioactivity was recovered in the secretion of the prostatic lobuli. A preferential accumulation of radioactivity in prostatic secretion was also observed 2 hrs. after intramuscular administration of ³H-estramustine to intact or castrated rats. In contrast, ³H-oestradiol and ³H-testosterone, which were also taken up by the ventral prostatic epithelium, were not recovered in prostatic secretion. The present results indicate that ³H-estramustine is secreted from the prostatic cells into the lumina of the prostatic lobuli. It is speculated that a recently detected estramustine-binding protein in rat ventral prostate may be involved in this process and that the present finding may be of relevance in the understanding of the mechanism of action of the chemotherapeutic agent Estracyt® (estramustine phosphate) used in the treatment of advanced prostatic carcinoma.     

WHO西太平洋地区医学索引收录期刊的数据提交能力分析

简要介绍WHO西太平洋地区医学索引（WPRIM）和当前WPRIM数据采集的途径及存在的问题,基于问卷调查,对WPRIM中国期刊从期刊信息数据提交能力、提交各方式倾向性和工作建议等方面进行分析,针对调查结果所反映的问题进行讨论,包括改进调查方式,提高WPRIM数据完整性,提升编辑部数据提交能力,推进WPRIM宣传工作等。     

Prevention of spinal cord ischemia in surgery of choraco-abdominal aneurysms The Bio Medicus pump, the recording of somatosensory evoked potentials and the impact on surgical strategy

Over a 15-month period 14 consecutive patients underwent surgery for extensive thoracoabdominal aneurysms with a nonheparinized centrifugally pumped (Bio Medicus) bypass and somatosensory evoked potential (SEP) recording. Six patients had a femorofemoral and eight patients a left atrial-common femoral artery bypass. The study aimed to assess the contribution of this approach to the prevention of spinal cord ischemia and the impact of the approach on surgical strategy. All patients survived operation; the 30-day and hospital mortality was 21% (three patients). Two patients became paraplegic. No major cardiac and renal complications occurred. Distal aortic perfusion allowed staged cross-clamping of the aorta in five (35.7%) patients, resulting in a significant reduction in mean spinal cross-clamp time: 23.4 min versus a calculated time of 47.2 min in the same patients if distal aortic perfusion had not been used (p ≤ 0.002). In one patient surgical strategy was adjusted during cross-clamping in order to reattach a critical intercostal artery. SEP-derived information confirmed the appropriateness of the surgical strategy in all cases. Whether the spinal cord was protected by this perfusion remained unproven. No bypass-related complications occurred. Distal aortic perfusion and concomitant SEP monitoring offer an improvement of surgical strategy and safer surgery.     

基于全局非靶向代谢组学对黄蜀葵不同部位成分的差异分析

目的 通过代谢组学分析黄蜀葵叶、茎、根、花中主要化学成分及其相对含量，为提高黄蜀葵的药用和经济价值提供科学基础。方法 使用超高效液相色谱-质谱联用法对黄蜀葵各部位代谢物进行定性分析，通过全局非靶向代谢组学方法分析比较黄蜀葵不同部位主要代谢物的相对含量。结果 黄蜀葵主要功效成分黄酮类物质在叶、茎、根中也有大量分布，其中标志性化合物金丝桃苷在黄蜀葵叶中的相对含量最高。其他类活性成分，如生物碱、香豆素、萜类、氨基酸等在黄蜀葵叶中的相对含量也较高。结论 除了黄蜀葵花，其他部位如叶、茎、根中也含有较多的活性成分，在提取利用其中的活性成分时可以考虑全株提取或有针对性地部位提取，以提高黄蜀葵的药用及经济价值，减少资源浪费。