基于超声波采集的无线数据传输的输液监控系统研制

介绍一种基于无线数据传输的静脉输液远程监控系统,可以完成对输液速度等参数的精确控制和人性化提示,并可实现中央监控、监护、管理等功能。系统的下位机采用超声波检测液滴的流量,实现控制、显示、存储,并通过无线方式与上位机实时通讯,实现远程监控。     

GpsOne远程心脏病急救系统的设计与实现

将GpsOne混合通信定位技术和专业医疗诊断用的心电图采集技术整合到嵌入式单片机系统中,设计了一套新型的基于N-tier体系结构的远程心脏病急救系统。该系统能为远程心脏病急救提供准确的定位和必要的心电图诊断信息。     

Distribution of Plasmodium falciparum genotypes in clinically mild and severe malaria cases in Orissa, India

A cross-sectional study was conducted in a malaria hyperendemic state of India to ascertain the distribution of Plasmodium falciparum genotypes in patients with mild (n=40) and severe (n=35) malaria. PCR and nested PCR were used to determine the glutamate-rich protein (GLURP), merozoite surface proteins 1 and 2 (MSP1 and MSP2) and knob-associated histidine-rich protein (KAHRP) for characterization of the parasite. The results indicate that (i) the 200bp allele of the MAD20 family of MSP1 and the 550bp allele of the 3D7 family of MSP2 show over-representation in severe malaria cases; (ii) the multiplicity of infection with respect to MSP2 alleles is significantly higher (P<0.001) in severe cases than in mild cases; and (iii) comparison with the findings of other studies leads to the conclusion that the distribution of P. falciparum genotypes between different clinical groups differs geographically.     

Neurophysiological field potential analysis by microcomputer

A system is described for digitizing, storing, displaying, analyzing, and creating hardcopy drawings of electrophysiological field potential data, based on the Apple II microprocessor. The system uses commercially available hardware and user oriented software written in BASIC.     

Evolution of the Solenoid-Actuated Left Ventricular Assist System: Integration with a Pusher-Plate Pump for Intra-Abdominal Implantation in the Calf

The performance of an implantable left ventricular assist system (LVAS) utilizing a pulsed solenoid energy converter and a pusher-plate blood pump has been characterized in vitro and in vivo. A microprocessor-based electronic control system makes the LVAS completely self-regulating over the range of operating conditions and provides considerable flexibility in various assist modalities. Over forty thousand hours of in vitro and in vivo operating experience has been accumulated with current systems, and significant progress has been achieved in system durability and reliability. A new toggle latch has provided nearly a year of failure-free operation on the bench, without measurable wear. Energy converter efficiencies of 50% have been demonstrated. In vivo evaluation has been highlighted by an animal experiment still in progress after nearly four months of fault-free, continuous synchronous pumping.     

医疗设备工作环境温度远程监测系统设计

目的实现医疗设备工作环境温度远程监测。方法通过C51单片机技术实现DS18B20温度传感信息采集,并用VB语言编写相应的操作软件。结果实现了医疗设备工作环境温度远程监测、报警及回顾等功能,避免工作环境温控失调造成停机甚至损坏电路板的事件发生。     

Promoter methylation of the RGC32 gene in nonsmall cell lung cancer

BACKGROUND:

Lung cancer is the leading cause of cancer‐related deaths worldwide. Epigenetic inactivation of certain genes by aberrant promoter methylation is recognized as a crucial component in the initiation and progression of lung cancer. Response gene to complement 32 (RGC32) has been identified as a cell cycle regulator induced by activation of complements; however, its role in carcinogenesis is still controversial.

METHODS:

The authors examined the methylation status in the promoter region of RGC32 gene in nonsmall cell lung cancers (NSCLCs) using a methylation‐specific PCR and correlated the results with clinicopathological features.

RESULTS:

RGC32 methylation was found in 45 of 173 NSCLCs (26.0%) and was related to the gene expression. RGC32 methylation was more frequent in females than in males (P<0.05). RGC32 methylation was not significantly associated with the prognosis of patients; however, when the patients were categorized by TP53 mutational status, the effect of RGC32 methylation on prognosis was significantly different between those with and without TP53 mutations (P = .005 [test for homogeneity]). Specifically, RGC32 methylation was associated with significantly worse survival in the cases with wild‐type TP53, whereas it exhibited a better survival outcome in the cases with TP53 mutations.

CONCLUSIONS:

The current findings suggest that methylation‐associated down‐regulation of RGC32 plays an important role in the pathogenesis of NSCLC, particularly in females. However, further studies with a large number of cases are needed to confirm the authors' findings. Cancer 2011. © 2010 American Cancer Society.     

通用复合数据巡检网络的构建

本文提出了一种复合数据采集网络的构建方法,采用视频图像识别技术和无线串行通讯技术对大量实验设备输出结果进行数据采集。系统通过对视频图像的分割和插值放大处理后得到字模图像,进而与字库图像比对后得到图像中的数符串。同时系统采用仿真串口实现对具体设备数据的读取,并通过构建的无线网络进行串行数据采集。本系统适用于对大量实验数据的自动检测与汇总。     

Suppression of WIF-1 through promoter hypermethylation causes accelerated proliferation of the aryl hydrocarbon receptor (AHR) overexpressing MCF10AT1 breast cancer cells

The cause for increased cell proliferation in AHR overexpressing breast cancer cells still remains unknown. Here we studied the molecular basis of aggressive cell proliferation of an AHR overexpressing and ERα functionally down-regulated MCF10AT1 cell line, designated as P20E, in comparison to a matched sub-line, P20C with normal AHR expression and ERα function. We found that a 4-day treatment of P20E cells with 5-aza-2′-deoxycytidine (AZ) caused a significant suppression of cell proliferation. Such an effect of AZ was accompanied with the significant recovery of ERα function. Among diagnostic markers of AZ-induced cellular changes we found conspicuous up-regulation of mRNA expression of Wnt inhibitory factor-1 (WIF-1), particularly in P20E. The possibility of AZ-induced demethylation on the promoter of WIF-1 gene was confirmed through methylation specific PCR assay. Such AZ-induced changes in P20E cells were also accompanied with the decrease in the binding of nuclear proteins to the ³²P labeled TRE (TCF response element) and the reduced accumulation of β-catenin protein in the cell nucleus, indicating the importance of Wnt/β-catenin pathway in maintaining the increased cell proliferation in P20E line over P20C line. The importance of WIF-1 in this regard has been validated by transfecting cells with siRNA against WIF-1, which caused an increase in cell proliferation. Moreover, artificial overexpression of ERα in both P20E as well as MDA-MB-231 cells increased the mRNA expression of WIF-1. Together these results support our main conclusion that the aggressive cell proliferation of this AHR-overexpressing sub-type of transformed mammary epithelial cells is assisted by the epigenetic suppression of WIF-1 gene, which is caused by the loss of ERα.