Total and free tissue factor pathway inhibitor in pregnancy hypertension.

OBJECTIVE: To clarify the role played by tissue factor pathway inhibitor (TFPI) in pregnancy hypertension. METHODS: Using enzyme-linked immunosorbent assays, hemostatic measurements were obtained for women with pre-eclampsia (n=51), nonproteinuric hypertension of pregnancy (n=62), postpartum pre-eclampsia 24 h after childbirth (n=31), and no hypertension (healthy pregnant controls, n=100). RESULTS: There was a significant increase in circulating free TFPI levels in women with pre-eclampsia (9.7+/-6.2 ng/mL) or nonproteinuric hypertension of pregnancy (8.3+/-5.3 ng/mL) compared with healthy controls (5.3+/-2.1 ng/mL). In women with pre-eclampsia the levels remained elevated after placental delivery (10.6+/-4.0 ng/mL). Free protein S levels were significantly higher in women with pre-eclampsia (40.0%+/-10.7%), nonproteinuric hypertension of pregnancy (37.1%+/-12.5%), or postpartum pre-eclampsia (39.3%+/-9.1%) than in healthy pregnant controls (32.2%+/-8.5%). CONCLUSION: Increased levels of the physiologically active free forms of TFPI and free protein S, 2 coagulation inhibitors, may protect women with pregnancy-induced hypertension from the risks of hemostatic activation.     

Detection of human papillomavirus in organs of upper genital tract in women with cervical cancer

In this study, we evaluated the presence of human papillomavirus (HPV) DNA in organs of the female upper genital tract, using nine hysterectomy and salpingo-oophorectomy specimens affected by HPV-positive invasive cervical carcinomas, to establish if cervical HPV infection can spread to upper tracts of the female genital system. HPV DNA was evaluated by polymerase chain reaction (PCR) in all cervical carcinomas as well as in all tracts of the genital system. Then, these data were compared with the results obtained from PCR study of five other hysterectomy and salpingo-oophorectomy specimens (control cases). The criteria used for selection of the control cases were informed consent of the patients for research at the time of surgery, absence of neoplasms, absence of any anatomic lesion caused by HPV in cervix, and external genitalia. All selected cases were squamous cervical carcinomas. PCR analysis revealed HPV DNA in all cases of cervical carcinoma. The HPV DNA was detected as weak positivity on PCR analysis in other organs of the genital system. However, the distribution of HPV DNA varied in the various cases and in the different tracts of the same hysterectomy and salpingo-oophorectomy specimen. We believe that the HPV DNA, detected as a weakly positive signal, in the upper genital tract of patients who have a cervical squamous carcinoma could be a reflection of a latent HPV infection, as well as a sign of the existence of micrometastases containing HPV DNA, which cannot be detected by conventional histologic techniques.     

Arsenic trioxide exposure to ovarian carcinoma cells leads to decreased level of topoisomerase II and cytotoxicity

The objective of this study was to investigate the effect of arsenic trioxide (As(2)O(3)) on topoisomerase II levels using western blotting method on MDAH 2774 ovarian carcinoma cell culture. Experimental designs were established to determine the cytotoxic effects of As(2)O(3) on MDAH 2774 cells and the IC50 (fatal dose for the 50% of cells) value. Cytotoxicity experiments were carried out using various concentrations of As(2)O(3). The 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and trypan blue dye-exclusion tests were used to evaluate cytotoxicity. Topoisomerase II expressions were investigated using western blotting method with various concentrations of As(2)O(3). Densitometric analysis of topoisomerase 2 bands was carried out using Quantity One 1-D analysis software (Bio-Rad USA, Life Science Research, Hercules, CA). IC50 value of As(2)O(3) was found to be 5 x 10(-6) M for MDAH 2774 cells. When the bands were evaluated, it was observed that there was a decrease in topoisomerase II levels in MDAH 2774 cells with increasing concentrations of As(2)O(3). It was also observed by the densitometric analysis that topoisomerase II expression ratios of MDAH 2774 cells were decreased by approximately 50% at this concentration. Topoisomerase II levels were significantly decreased with the increasing concentrations of As(2)O(3). Inhibition of topoisomerase II enzyme was one of the antiproliferative influence mechanisms of As(2)O(3).     

根管细菌渗漏体外模型的建立

目的：建立检测微细菌培养模型，以用于评价根管充填封闭情况。方法：牙根的冠根两端各连接一细菌培养储室，冠方储室置1mL无菌培养基并接种0．1mL菌液，根方储室起始为无菌培养基。每隔3d将冠方储室中菌液吸出1mL，并注入1mL新鲜BHI培养基，共90d。当根尖储室中培养基发生浑浊即为细菌渗漏发生，记录细菌渗漏发生时的天数。选择18个直根管前牙，其中牙胶充填组6个，用冷牙胶侧方加压充填技术充填；空管组6个，根管只预备不充填；根管密封组6个，根管充填并严密封闭根管口和根尖孔。用设计的模型检测密封状况。结果：全部空管组6个样本在1d内根方储室中出现浑浊；根管密封组6个样本的根方储室在90d内均未发生浑浊。牙胶充填组有2个样本分别在44d和46d发生浑浊。结论：此模型方法简单易行且实用有效，具有一定的临床相关性和可行性。     

Effects of antisense mediated inhibition of cathepsin K on human osteoclasts obtained from peripheral blood.

Cathepsin K is a cystein protease that displays a proteolytic activity against Type I collagen and is abundantly and selectively expressed in osteoclasts where it plays a critical role in bone degradation. Its direct role in bone tissue has been defined by knock-out mice studies and inhibiting strategies in animals models. However, direct proof of cathepsin K function in human osteoclast model in vitro is lacking. The aim of this study is to analyze cathepsin K expression and localization in human osteoclasts obtained from peripheral blood and to examine cathepsin K function in these cells by antisense oligodeoxynucleotide (AS-ODN) strategy. AS-ODN was added to the culture of osteoclast precursors induced to differentiate by RANKL and M-CSF. AS-ODN treatment produced a significant down-regulation of cathepsin K mRNA (>80%) and protein expression, as verified respectively by Real-time PCR and by immunocytochemistry or Western blot. The cathepsin K inhibition caused an impairment of resorption activity as evaluated by a pit formation assay ( p = 0.045) and by electron microscopy, while the acidification process was unaffected. We demonstrated that antisense strategies against cathepsin K are selectively effective to inhibit resorption activity in human osteoclasts, like in animal models.     

植入式心律转复除颤患者的护理及随访

目的总结植入式心律转复除颤器(ICD)安置术的护理。方法2001年7月~2004年6月给予13例ICD患者进行了护理及随访。结果术后有2例患者出现心律失常“风暴”,经处理后未再出现心室颤动。随访患者1~4年,均存活,未发生晕厥等并发症。结论术前重视患者的心理护理,术后密切观察病情,积极预防心律失常“风暴”,并做好ICD健康教育,是ICD手术成功的重要护理措施。     

聚对苯撑乙炔的合成及光谱性能-共轭主链被间位苯环阻隔

报道了一种阻隔型聚对幕撑乙炔((PPE)的合成与电致发光特性，合成的模型小分子证明，在聚对苯撑乙炔(PPV)主链引入间位幕环，可阻隔PPV的有效共轭长度，改变其发光颜色，研究了电致发光暑件ITO／m-PPE／Al的电致发光特性。     

红花注射液治疗冠心病心绞痛、心肌缺血临床观察

目的:观察红花注射液治疗冠心病心绞痛、心肌缺血的临床疗效。方法:选择126例冠心病心绞痛、心肌缺血的病例,分红花组及丹参组,疗程两周,详细记录治疗前后的临床表现、心电图、血糖、血脂、血液流变学检查的变化。结果:红花、丹参均可明显改善冠心病心肌缺血相应的临床症状和心电图改变,以红花较显著。红花还有降血糖、血脂作用,显著改善血液流变指标。结论:红花注射液是治疗冠心病心绞痛、心肌缺血的理想药物。     

传染性非典型肺炎患者泪液和血液中特异性抗体检测的对比研究

目的：通过传染性非典型肺炎(SARS)患者泪液病毒特异性抗体的检测，寻找SARS病毒是否通过泪液或眼结膜传播的理论依据，了解泪液和血液中SARS病毒特异性抗体检测结果的一致性程度。方法：用酶联免疫吸附法(ELISA)检测18例不同病程的SARS患者泪波及血清SARS病毒特异性抗体。结果：18例SARS患者泪液中的特异性IgM类和IgG类抗体均为阴性，而同组患者有13例其血液中的特异性IgM类和IgG类抗体呈阳性。结论：SARS病毒可能不通过泪液或结膜传播。