Ovarian stromal luteoma in a patient with endometrial carcinoma

A 65-year-old patient with endometrial adenocarcinoma underwent hysterectomy. Pathological examination of the right ovary revealed a coexisting stromal luteoma. The possible relationship between the two neoplasms as well as the intriguing pathogenesis and controversial nomenclature of ovarian steroid cell tumors are discussed. The incidental discovery of a stromal luteoma in a total hysterectomy specimen removed for endometrial carcinoma raises interesting questions about the perceived innocuous nature of these rare lipid cell tumors.     

Pap smear screening history of women with squamous cell carcinoma and adenocarcinoma of the cervix

BACKGROUND: Since the introduction of the Pap smear screening, the incidence of squamous cell carcinoma (SCC) has decreased significantly, but the incidence of adenocarcinoma (AC) relative to SCC has increased. AIM: To compare the Pap smear history of patients with AC and SCC of the cervix. METHODS: Patients for the study were identified from the database of Queensland Centre for Gynaecological Cancer. Patients with AC and SCC were matched for age at diagnosis and International Federation of Gynecology and Obstetrics stage. The final population included 188 matched pairs, being 376 patients in total. Data were collected upon the histological type of cancer, result of the most recent Pap smear, date and result of the Pap smear prior to the most recent Pap smear and symptoms. Chi-squared tests and Fisher's exact test were used to compare the two patient groups for several variables. RESULTS: Patients with AC had significantly more false-negative results on their most recent Pap smear (P<0.0001) than patients with SCC. The incidence of symptoms such as bleeding and/or vaginal discharge was comparable in patients with AC and SCC. The time between the most recent Pap smear and the diagnosis of cervical cancer was significantly shorter for patients with AC (P=0.01). CONCLUSIONS: Patients with AC had Pap smears more regularly than those with SCC, and their most recent Pap smear was significantly more likely to be normal. Thus, Pap smear prior to a diagnosis of AC is more likely than SCC false-negative and therefore not indicative of cervical cancer.     

A case of pulmonary type of ovarian small cell carcinoma

Small cell carcinoma is a rare form of ovarian cancer with a poor prognosis. It is divided into two types, the hypercalcemic and the pulmonary type, of which the latter is extremely rare. A 49-year-old woman presented with an acute abdomen and was suspected to have torsion of a left ovarian tumor, which was followed up with an emergency operation. Postoperative pathological examination gave a diagnosis of the pulmonary type of ovarian small cell carcinoma. Six courses of paclitaxel and carboplatin therapy were given as adjuvant chemotherapy. The patient has survived for 36 months without recurrence. Here we present an extremely rare patient with the pulmonary type of ovarian small cell carcinoma.     

红细胞输注效果的影响因素分析

目的分析红细胞输注无效的原因,为临床红细胞的安全有效输注提供依据。方法回顾性总结分析本院半年内无效输血与可能因素之间的关系。结果红细胞无效输注与性别、年龄无关,多见于血液病和地贫患者,与既往输血次数(妊娠一次计算为一次输血)、红细胞制品贮存时间以及红细胞制品品种有关。结论尽量减少输血次数,正确选用新鲜的红细胞制品可降低红细胞输注无效率。     

体外导入外源IL-10基因对HSC增殖的影响

目的利用重组复制缺陷型腺病毒AdIL 10将外源IL 10基因导入肝星状细胞 (HSC) ,并观察其对HSC增殖的影响。方法将本实验室自行构建的重组腺病毒质粒pAdIL 10用Lipofectamin包装转染HEK 2 93细胞 ,即可获得重组腺病毒AdIL 10。应用胶原酶原位灌注和密度梯度离心法分离大鼠HSC ,当HSC生长至 80 %密度时 ,以 5× 10 4细胞 /孔接种于 96孔板 ;HSC培养 2 4h后 ,取病毒AdIL 10以感染复数 (MOI) 5 0感染HSC ;用MTT法检测HSC增殖率。结果pAdIL 10经 2 93细胞包装 ,3d后可观察到绿色荧光蛋白 (GFP)表达 ;AdIL 10感染HSC 3d后可观察到GFP表达 ,且HSC增殖受到抑制。结论重组腺病毒AdIL 10感染HSC后可抑制其增殖     

小脑组织移植的实验研究

应用不同胎龄的大鼠小脑组织(E_(15)和E_(18))植入受体鼠小脑,成活1～2个月后处死动物。光镜下观察移植物成活情况。移植物总成活率为35％。E_(15)成活率(60％)高于E_(18)(10％)。所有成活的移植物可根据细胞结构的特点易与受体脑组织区别,镜下可见到良好分化及成熟的神经细胞。     

慢性脑血流灌注不足认知功能障碍与环胞素A治疗作用的实验研究

目的 探讨慢性脑血流灌注不足认知功能障碍和环胞素Ａ治疗作用。方法 持久性双侧颈总动脉结扎所致慢性脑血流灌注不足的大鼠模型;用电脑控制穿梭箱记录大鼠学习记忆能力;用免疫组化研究脑内血源性白细胞和Ｔ细胞浸润的规律、分布和可能作用及免疫抑制剂环胞素Ａ（ＣｓＡ）的治疗效果。结果 慢性脑血流灌注不足２～４个月大鼠的学习记忆能力明显下降;缺血１个月后皮层、海马和白质有白细胞和Ｔ细胞的浸润,２～４月浸润的白细胞