Preliminary study of virtual touch tissue imaging quantification in diffuse testicular diseases of male infertility

Objective To explore the preliminary application method and diagnostic value of virtual touch tissue imaging quantification (VTIQ) in diffuse testicular diseases of male infertility. A total of 70 male examiners in our hospital from June 2019 to March 2020 were selected as the research objects. According to whether the subjects are sick (testicular microlithiasis, varicocele), all subjects are divided into three groups: normal group, testicular microlithiasis group, and varicocele group. Whether there is a difference between the groups, and analyze whether there is a correlation between the index reflecting the quality of the semen and the testicular shear wave velocity. The measurement of different positions in the testis will affect the magnitude of the shear wave velocity (P < 0.05). The shear wave velocities of the testes of different diseases are obviously different from the normal side. At the same time, postural changes will have a certain impact on the shear wave velocities of both testes (all P < 0.05). There is a certain correlation between the testicular shear wave velocity and the conventional semen quality index (total sperm motility (P < 0.05)) of the subjects. Acoustic palpation tissue imaging quantitative technology can be applied to the diagnosis of male testes and testicular-related diseases (especially diffuse testicular diseases), and is more suitable for male infertility, which can provide clinical diagnosis and treatment options certain imaging basis.     

高能冲击波对骨组织代谢及愈合影响的实验及临床研究

目的了解高能冲击波对骨组织ＤＮＡ的影响，以及其治疗骨折延期愈合、骨折不连接的疗效。方法通过新西兰种兔肢体实施体外高能冲击波治疗，取骨膜组织进行体外培养Ｈ３—ＴｄＲ掺入放射性自显影研究。临床应用其治疗骨不连接１８例，骨折延迟愈合２０例。结果证实冲击后１～２周实验侧骨膜组织Ｈ３ＴｄＲ标记率明显高于对照侧（Ｐ＜０．０５），表明实验侧骨膜成骨细胞有丝分裂明显增强，骨膜成骨作用增强。临床冲击治疗后１６周，２０例骨折延迟愈合均愈合；冲击后１６周，１８例骨不连接，１５例愈合。结论该法可促进骨膜形成、骨细胞ＤＮＡ代谢，是治疗骨折延迟愈合、骨不连接的有效方法，其作用机理仍有待于进一步研究。     

运用彩色多普勒流速剖面图测定血流量的实验研究

运用实验血流模型，检测新近开发的彩色多普勒血流速度部面图（velocityprofile，VP）对血流量测定的准确性，并与脉冲型多普勒（PulsedwaveDoppler，PWD）测定法比较。结果显示在不同流速下两种方法的流量测值与实际流量间均有高度相关性，相关系数r分别为0．999（P＜0．001）和0．988（P<0．02）。但测得流量与实际流量之间的误差程度，VP法为-7.64～2．79％，PWD法为17.82～27.97％。表明VP法较PWD法更接近实际情况。     

人肝癌SMMC-7721细胞热休克反应时糖皮质激素受体mRNA减少机制初探

目的 探讨人肝癌SMMC-7721细胞热休克反应时糖皮质激素受体（GR）mRNA减少的机制。方法 利用聚合酶链反应（PCR）从大鼠GRcDNA质粒及人肝癌SMMC7721细胞基因组中扩增大鼠GR跨外显子3，4，5片段（与人GR跨外显子3，4，5片段同源性在89%以上）及人GR内含子E的DNA片段，构建转录质粒，并在体外进行转录，用地高辛标记反义RNA探针，进行细胞原位杂交，碱性磷酸酶底物NBT-BCIP显色。计算机图像分析系统进行扫描半定量分析。结果 人肝癌SMMC-7721细胞44℃培养1h后，用跨外显子和内含子探针杂交显色所得的积分灰度值均比未经热休克反应组低；加放线菌素D后热休克反应组，用跨外显子探针杂交显色所得的积分灰度值比单加放线菌素D组低，而用内含子探针杂交显色所得的积分灰度值比单加放线菌素D组高；加放线菌酮后热休克反应组，用跨外显子探针和内含子探针杂交显色所得的积分灰度值均比热休克反应组高。结论 热休克反应时GRmRNA转录过程受抑，GRmRNA前体成熟过程存在剪接障碍，GRmRNA降解加快。     

Electrophysiologic Basis for T Wave Alternans as an Index of Vulnerability to Ventricular Fibrillation

Electrophysiologic Basis for T Wave Alternans. Substantial evidence indicates that T wave alternans is an intrinsic property of ischemic myocardium. The electrophysiologic basis appears to be spatial and temporal heterogeneity of repolarization resulting from changes in action potential morphology rather than in activation sequence. Ischemia-induced changes in postrepolarization refractoriness and depressed electrical restitution of action potential duration have also been implicated. The main underlying ionic basis for T-wave alternans during coronary occlusion appears to be derangements in intracellular cycling of calcium. Accumulation of potassium in the extracellular space adjoining ischemic cells and disruption in electro-genie sodium-calcium exchange may also be involved. In humans, T wave alternans has been observed in Prinzmetal's and classical angina, angioplasty, and bypass graft occlusion. Under these conditions associated with acute myocardial ischemia, alternans is restricted to the ischemic zone, and alternation in action potential morphology is an underlying factor. Recently, repolarization alternans has been shown to be a statistically significant predictor of the results of electro physiologic testing and arrhythmia-free survival in individuals with and without organic heart disease. Collectively, these observations provide a rationale for quantitation of T-wave alternans magnitude for assessment of vulnerability to life-threatening ventricular arrhythmias both in response to and independent of the effects of myocardial ischemia.     

心房颤动患者心房活动特征的提取与分析

目的构造一种新的盲源分离方法,从心房颤动(简称房颤)患者心电图中有效提取心房活动信号,在此基础上分析心房活动的特征指标。方法采用小波变换来实现心电信号的稀疏表示,证明了独立分量分析(ICA)的3个基本条件在小波域依然满足,建立了小波域心电信号的独立分量分析的数学模型。结果应用小波域独立分量分析模型可以有效提取心房活动信号。讨论了与房颤相关的P波离散度、频谱、房颤周长等几个预测指标的具体实现,比较了不同房颤的具体特征。结论该算法可以有效提取房颤特征信号,对房颤机制的深入研究以及临床治疗具有很好的借鉴意义。     

Effects of short-term exposure to fine and ultrafine particles from indoor sources on arterial stiffness – A randomized sham-controlled exposure study

ObjectivesParticulate air pollution is linked to adverse cardiovascular effects, including arterial stiffness. The aim of the study was to investigate the effect of short-term exposure to indoor fine and ultrafine particles on augmentation index (AIx), augmentation pressure (AP), and pulse wave velocity (PWV), early signs of vascular damage.MethodsWe analyzed the association of particle emissions from typical indoor sources (candle burning - CB, toasting bread - TB, and frying sausages - FS) with changes in pulse wave analysis indices in 55 healthy adults in a randomized cross-over controlled exposure study. Particle mass concentration (PMC), size-specific particle number concentration (PNC) and lung-deposited particle surface area concentration (PSC) were measured during the 2 h exposure. AIx and AP were measured before, directly, 2, 4 and 24 h after exposure. PWV was measured directly and 24 h after exposure. We performed multiple mixed linear regression analyses of different particle metrics and AIx, AP and PWV.ResultsThe highest mean PMC was observed during FS reaching a maximum of 210 μg/m³ PM₁₀. The maximal PNC for UFP <100 nm was reached during CB with 2.3 million particles/cm³. PSC was similar across all three exposures (about 3000 μm²/cm³). Strongest associations between different particles metrics and arterial stiffness indices could be observed for UFP from CB and FS and for PMC from TB. The highest mean increase could be observed for the UFP fraction <10 nm, measured during CB, and AIx with an increase of 9.5%-points (95%-CI: 3.1; 15.9). PSC seemed to follow the pattern of PNC. PM₁₀ and PM_2.5 from TB led to clear changes in AIx with biggest increases for PM₁₀ of 5.8%-points (95%-CI: 3.2; 8.4) 2 h after exposure and for PM_2.5 of 8.1%-points (95%-CI: 2.5; 13.7) directly after exposure.ConclusionsOur study indicates effects of indoor exposure to fine and ultrafine particles on systemic arterial stiffness indices that depend on the indoor source as well as on particle metric. Differences in size-specific physical characteristics of source-specific particles might account for these differential effects. We did not observe clear and stable associations of indoor particle exposure and PWV.