淫羊藿苷对小鼠破骨细胞MMP-9、CK mRNA表达的影响

目的观察淫羊藿苷对体外培养小鼠破骨细胞MMP-9、CK mRNA表达的影响。方法用浓度分别为25 ng/ml、30 ng/ml、10-8mol/L的巨噬细胞集落刺激因子(M-CSF)、核因子κB受体活化因子配体(RANKL)、1,25(OH)2VitD3体外诱导培养小鼠骨髓源性破骨细胞,分别加入0 mol/L、10-5mol/L的淫羊藿苷,培养48 h后,抽提总RNA,采用半定量逆转录-聚合酶链反应(RT-PCR)方法检测破骨细胞中基质金属蛋白酶-9(MMP-9)、组织蛋白酶K(CK)mRNA表达的变化。结果与空白对照组比较,10-5mol/L的淫羊藿苷可明显下调MMP9mRNA的表达(P0.05),但对CK mRNA的表达无明显影响(P0.05)。结论淫羊藿可以通过下调MMP-9基因表达,从而抑制破骨细胞的分化形成及骨吸收。     

胶质瘤患者肿瘤组织和血浆中MMP-9的表达及意义

目的探讨胶质瘤患者肿瘤组织和血浆中基质金属蛋白酶-9(MMP-9)的表达、两者之间的关系及意义。方法收集40例胶质瘤患者病理标本、患者术前血浆及临床资料,分成高度恶性组和低度恶性组。10例因重度颅脑损伤而行内减压手术切除的脑组织和患者术前血浆,作为对照组。运用免疫组织化学染色法对胶质瘤组织标本中MMP-9表达进行半定量分析。运用酶联免疫吸附测定法检测血浆中总MMP-9浓度。结果 MMP-9在正常脑组织中无表达,高度恶性组瘤组织中MMP-9的表达显著高于低度恶性组(P=0.027)和对照组(P=0.000)。各级别胶质瘤组MMP-9血浆浓度均显著高于对照组(P0.001)。瘤组织MMP-9强阳性表达组血浆MMP-9浓度显著高于阴性组(P=0.022)和弱阳性组(P=0.048),血浆MMP-9浓度与相应肿瘤组织MMP-9的表达水平呈正相关(rs=0.530,P=0.000)。结论瘤组织MMP-9表达与胶质瘤恶性程度呈正相关,其高表达可能与胶质瘤的侵袭转移有关;胶质瘤患者血浆MMP-9浓度一定程度上反映出瘤组织中MMP-9表达水平。     

Hyperbaric oxygen therapy improves local microenvironment after spinal cord injury

Clinical studies have shown that hyperbaric oxygen therapy improves motor function in patients with spinal cord injury. In the present study, we explored the mechanisms associated with the recovery of neurological function after hyperbaric oxygen therapy in a rat model of spinal cord injury. We established an acute spinal cord injury model using a modification of the free-falling object method, and treated the animals with oxygen at 0.2 MPa for 45 minutes, 4 hours after injury. The treatment was administered four times per day, for 3 days. Compared with model rats that did not receive the treatment, rats exposed to hyperbaric oxygen had fewer apoptotic cells in spinal cord tissue, lower expression levels of aquaporin 4/9 mRNA and protein, and more NF-200 positive nerve fibers. Furthermore, they had smaller spinal cord cavities, rapid recovery of somatosensory and motor evoked potentials, and notably better recovery of hindlimb motor function than model rats. Our findings indicate that hyperbaric oxygen therapy reduces apoptosis, downregulates aquaporin 4/9 mRNA and protein expression in injured spinal cord tissue, improves the local microenvironment for nerve regeneration, and protects and repairs the spinal cord after injury.     

山东省首起人感染H7N9禽流感确诊病例感染因素分析

目的通过对山东省首次出现的2例人感染H7N9禽流感病例的调查分析,为制定人感染H7N9禽流感预防控制措施提供科学依据。方法 2013年4月采用流行病学、临床检查、血清学监测和Real-time PCR法进行分析和诊断。结果 2例确诊病例均无明显的病、死禽接触史,呈现出家庭聚集性,其他的密切接触者均未发现异常,结合基因序列分析,提示可能存在有限的人传人。结论 2例人感染H7N9病例的感染来源不明确,需加强不明原因肺炎监测和进一步研究有关危险因素。