戊型肝炎病毒感染合并神经系统损害1例报告及文献复习

目的 总结分析戊型肝炎病毒(HEV)感染并发神经系统病变的临床特点,提高对神经系统损害这一戊肝病毒感染少见肝外表现的认识.方法 报告1例戊型肝炎病毒感染并发急性颈神经根炎病例.并检索1995-2006年国内外报道的戊型病毒性肝炎并发神经系统病变患者10例,进行总结.结果 口11例患者中,男6例.女5例,平均年龄36.1岁;吉兰-巴雷综合征(GBS)5例,脑膜脑炎2例,急性横贯性脊髓炎1例,动眼神经麻痹1例,Bell麻痹1例,急性颈神经根炎1例.除脑膜脑炎外,前驱感染至出现神经系统症状平均间隔13.1d,平均16.3d达高峰.所有患者均有急性病毒性肝炎表现,肝功能平均在5.7周内恢复正常.5例患者接受糖皮质激素或静脉免疫球蛋白、血浆置换治疗,其余均行对症支持治疗.多数患者(9/11)神经系统症状可完全恢复,平均病程7.3周.结论 神经系统损害可能是急性戊型肝炎的肝外表现之一,多为自限性,合并神经系统表现的HEV感染的病程相对单纯肝脏受累病程较长.     

Prevalence of hepatitis E virus infection in multiple transfused Brazilian patients with thalassemia and sickle cell disease

Hepatitis E virus (HEV) is a leading cause of acute hepatitis worldwide. The virus is acquired by fecal-oral route; however, it can also be transmitted by blood transfusion. The objective of the study was to examine anti-HEV immunoglobulin G and HEV RNA prevalence in multiple transfused patients with thalassemia and sickle cell disease (SCD), and in blood donors. The HEV seroprevalence in the patients was 13% (20% in thalassemics; 7.7% in SCD), and 11% in blood donors. No positive result for HEV RNA was obtained. This is a pioneer study examining HEV circulation in Brazilian patients with hemoglobinopathies.     

抗-HEV单克隆抗体B4C重链可变区基因的克隆及初步表达

抗-HEV87A株单克隆抗体（mAb）B4C经体外研究鉴定,证实对我国暴发和散发性HEV毒株有很高的特异性。为从分子水平上了解mAbB4C,我们利用基因工程技术,从杂交瘤细胞系中克隆出了重链可变区基因,并对其全部核苷酸序列进行了分析,可能属于小鼠免疫球蛋白重链IA亚组。将VHcDNA克隆到高效表达载体pQE－31中,经IPTG诱导,SDS－PAGE分析,可见1条Mr为16000左右的表达带,表达产物占菌体总蛋白的29．3％,主要以包涵体形式存在。     

戊型肝炎病毒与ING5相互作用的研究

目的 构建ING5基因真核表达载体和ING5荧光素酶载体,将pMIR-Report-ING5荧光素酶质粒与PUC-HEV质粒共转染293T细胞后,验证HEV与ING5之间相互关系。方法 通过提取293T细胞总RNA,RT-PCR法扩增ING5基因序列,并克隆到真核表达载体PGC3.1 (+)上。利用脂质体转染法将PGC-ING5真核表达质粒转染HepG2细胞后,通过 Western Blot法检测ING5表达情况;将pMIR-Report-ING5荧光素酶质粒和PUC-HEV质粒共转染293T细胞进一步探究ING5与HEV之间相互作用关系。结果 经双酶切和测序鉴定真核表达质粒PGC-ING5构建成功,ING5基因在HepG2细胞中成功表达;将pMIR-Report-ING5荧光素酶质粒与PUC-HEV质粒共转染293T细胞后,发现HEV明显抑制239T细胞中pMIR-Report-ING5荧光素酶的活性。结论 成功构建了PGC-ING5真核表达载体和pMIR-Report-ING5荧光素酶载体,并且证明HEV与ING5之间具有相互作用。     

猪HEV实验感染SD大鼠的研究

目的探索SD大鼠对猪HEV的易感性。方法22只SD大鼠,随机分为4组,每组均设一只对照。前两组实验鼠分别经尾静脉注射0.6×102 GE 和1.2×102 GE猪HEV 4型病毒液（病毒滴度为102 GE/mL）,其余两组实验鼠分别经灌胃途径给予0.6×102 GE 和1.2×102 GE病毒液。每组的对照鼠均以相应途径给予相应剂量的PBS。在注射前及注射后分别采集鼠血清和粪便标本,用RT nPCR法检测HEV RNA及ELISA法检测血清抗 HEV总抗体;且当血清ALT水平升高后取鼠肝组织通过光镜和电镜检查病理学变化。结果静脉注射大剂量病毒组的4只SD大鼠在感染2周后血清ALT明显升高,其中3只肝组织病理学检查出现轻微的非特异性炎症反应,有1只鼠出现抗 HEV抗体阳转;其他组的鼠感染后未见异常改变。所有血清标本和粪便标本均未检测出HEV RNA。结论猪HEV可能会跨种系传播感染SD大鼠但易感性较低且需较大的感染剂量。     

Analysis of Avian Hepatitis E Virus from Chickens, China

Avian hepatitis E virus (HEV) has been identified in chickens; however, only 4 complete or near-complete genomic sequences have been reported. We found that the near-complete genomic sequence of avian HEV in chickens from China shared the highest identity (98.3%) with avian HEV from Europe and belonged to avian HEV genotype 3.     

Hepatitis E virus infection in sheltered homeless persons, France

To determine the prevalence of hepatitis E virus (HEV) infection among sheltered homeless persons in Marseille, France, we retrospectively tested 490 such persons. A total of 11.6% had immunoglobulin (Ig) G and 2.5% had IgM against HEV; 1 person had HEV genotype 3f. Injection drug use was associated with IgG against HEV.