Characterization of chemotherapy-induced morphonuclear modifications in the P388 leukaemia and the MXT mammary tumour models of the mouse

Chemotherapy-induced morphonuclear modifications were monitored in vivo by means of the digital cell image analysis of Feulgen-stained nuclei. Two experimental models were used, i.e. the P388 mouse leukaemia and the MXT mouse mammary carcinoma. The drugs used were doxorubicin, etoposide and cyclophosphamide. The results indicate that the chemotherrapy induced a significant decrease in the MXT tumour growth and a significant increase in the survival of the P388 leukaemic mice. These effects were accompanied at the morphonuclear level by an increase in the nuclear area, by modifications in the DNA content in accordance with the effects of the drugs on the cells cycle and by several modifications in the chromatin texture in accordance with the effects of the drugs on the cells cycle and by several modifications in the chromatin texture in accordance with the model or drugs studied. While there were neither homogeneous morphonuclear changes in all treatment groups nor clearcut correlations between the morphonuclear changes and tumour growth or the survival of the animals, the present study nertherless shows that it is possible, at least partly, to monitor in vivo certain chemotherapy-induced effects occurring at the morphonuclear level, and subsequently to obtain information on the mode of action of the drugs.     

旋毛虫肌幼虫囊包染色标本制作方法的改进

目的探讨旋毛虫肌幼虫囊包染色标本的制作方法。方法采用醋酸卡红染色法制作旋毛虫肌幼虫囊包标本。结果醋酸卡红染色的标本,囊包轮廓清晰,囊内幼虫透明度及清晰度高,体态自然,立体感较强。结论醋酸卡红染色可用于旋毛虫肌幼虫囊包永久标本的制作。     

FRD上皮组织特殊染色液在宫颈病变筛查中的应用

目的：探讨叶酸受体介导的宫颈特殊染色法(FRD)上皮组织特殊染色液在宫颈病变检查中的临床价值。方法选取2014年5月至2015年11月就诊于松岗人民医院妇产科门诊行宫颈病变筛查的受检者100例,均同时给予FRD宫颈特殊染色检测和液基细胞学(TCT)检测,两种检测中任何一项提示阳性则进一步行阴道镜宫颈活检,并将检测结果与病理结果进行比较。结果与病理诊断结果比较,FRD检测的敏感性、特异性、阳性预测率、阴性预测率、误诊率、漏诊率分别为80.72%、84.21%、75.00%、90.14%、26.88%、20.00%,TCT检测的结果则分别为83.12%、83.12%、73.47%、87.67%、25.79%、19.28%,两种检测方法所得数据比较差异均无统计学意义(P>0.05)。结论 FRD上皮组织特殊染色液检测和TCT检测在宫颈病筛查中的临床价值相当,但采用FRD上皮组织特殊染色液检测具有操作简单、诊断全面、成本低廉以及结果快速等优点,值得临床推广。     

Assessment of lymphocyte proliferation for diagnostic purpose: Comparison of CFSE staining,Ki-67 expression and 3H-thymidine incorporation

The capability of lymphocytes to respond to antigenic or mitogenic stimulation is an important feature in the diagnosis of various immunodeficiencies and immune disorders. We used large cohorts of both immune compromised patients and healthy controls to measure lymphocyte proliferations by means of three methods: CFSE staining, Ki-67 expression and ³H-thymidine incorporation. The advantages and disadvantages of each method was then evaluated for use in routine clinical diagnostic. The statistical analysis was performed between the outcomes and the correlation between all three methods was computed. CFSE and Ki-67 assay correlated well with the r = 0.767, correlation between Ki-67 expression and ³H-thymidine incorporation was 0.546 and correlation between CFSE staining and ³H-thymidine incorporation was 0.337. The differences between these three methods concerning complexity, sensitivity and reliability as well as the financial aspects are discussed hereafter. CFSE and its analogues provide the cheapest and reasonable choice for measuring lymphocyte proliferation, while Ki-67 represents a more expensive, but more sensitive and robust method. The original ³H-thymidine assay does not bring any advantages and cannot compare to the competition presented by modern flow cytometric methods available today.     

Syringoma: a clinicopathologic and immunohistologic study and results of treatment

The purpose of our study was to describe clinical and histopathological features of sixty one patients with histological diagnosis of syringoma over four year period in our dermatology clinic in Korea. Female:male ratio was 6.6:1 with onset of age during 2nd and 3rd decades in more than half of the patients in our study. The most frequently involved site was eyelids (43 cases, 70.5%) and the most common color of lesion was skin-color (30 cases, 49.2%). In 34 cases, characteristic tad-pole appearances (55.7%) were observed. Basal hyperpigmentation was observed more frequently in brown-colored lesion (p=0.005). Fibrosis was observed more frequently in erythematous lesion (p=0.033). Keratin cyst was observed less frequently in genital involved group (p=0.006). We also performed immunohistochemical stain for the presence of progesterone receptor (PR) and estrogen receptor (ER) in fifty six cases with negative results.