胃癌第一站淋巴结窦组织细胞增生生发中心增生对胃癌预后的影响

目的 :研究胃癌第一站淋巴结窦组织细胞增生 (SH)、生发中心增生 (GCH)对胃癌第一站淋巴结转移及预后的影响。方法 :将SH、GCH分别分为 - ,+ ,+ +三级 ,分析SH ,GCH对胃癌第一淋巴结转移的影响 ,比较其 5年生存率。结果 :①SH -患者淋巴结转移率明显比SH + ,SH + +高 (P <0 .0 0 5 ) ;5年生存率随SH分级数升高而升高 (P <0 .0 0 5 ) ;②GCH -患者淋巴结转移率明显比GCH + ,GCH+ +高 (P <0 .0 0 5 ) ;5年生存率随GCH分级数升高而升高 (P <0 .0 1)。结论 :SH ,GCH患者胃癌第一站淋巴结转移率较低 ;SH ,GCH分级数越高预后越好 ;SH ,GCH可作为预测胃癌预后的指标之一。     

人血浆高敏感性C反应蛋白与代谢综合征相关性分析

目的:了解我国人群血浆高敏感性C反应蛋白(hs-CRP)水平与代谢综合征(MS)关系及其它影响hs-CRP水平的相关因素。方法:1198例血浆标本来自2006年3月~2006年11月在浙江省桐乡市第三人民医院体检人群。采用Beckm an LX20血生化全自动生化仪及其配套试剂,测定上述空腹血浆标本中血糖(FPG)、甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)和hs-CRP水平。MS诊断标准参照中华医学会推荐标准。采用SPSS13.0统计学软件,分析hs-CRP水平与MS关系及其它影响hs-CRP水平的相关因素。结果:男性hs-CRP水平(x±s=1.55±0.53)高于女性(x±s=1.32±0.56)(P<0.001)。15.3%被检者(183/1198)患有MS,其hs-CRP水平明显高于非MS者(P<0.001)。各MS判断指标中,分别有肥胖、高血压病、糖尿病、高TG、低HDL-c时,其hs-CRP水平也明显升高(P<0.001)。排除年龄、性别影响后,hs-CRP水平与MS显著相关(OR值2.18,P<0.001)。结论:hs-CRP水平与MS密切相关,hs-CRP水平增高可作为独立反映MS发生的标志性检测指标。     

不同起搏模式对病人生存质量影响的调查研究

[目的]探讨不同起搏模式对病人生存质量（QOL）的影响。[方法]以健康调查问卷（SF一36量表）对112例不同起搏模式病人进行问卷调查，比较3种起搏模式下病人的QOL水平。[结果]除机体疼痛、角色情绪2个维度外其余6个维度（生理功能、角色限制、活力、社会功能、精神健康、总体健康）3种起搏模式间比较差异有统计学意义（P〈0．05）。[结论]应用AAI型、DDD型起搏器的病人生存质量优于VVI型起搏。     

云南罗平医院员工对医院的认同度分析

通过问卷调查和访谈分析,调查了云南罗平医院194名员工对医院的认同度,调查对象包括行政、临床和后勤人员,从医院管理模式、医院绩效考核、医院薪酬待遇等方面用描述统计方法分析了数据,显示了医院员工的认同度情况及管理中存在的问题,并分析了不同因素对医院员工的认同度影响,为进一步改善医院管理状况,提高管理水平提供了参考。     

四类抗感染药物不良反应相关因素的探讨与分析

目的：使输液疗法中药物不良反应降到最低限度，使患者获得安全、有效的治疗，同时，也是对有限药物资源的维护。方法：对用四类抗感染药物出现不良反应91例患者行减慢输液速度、平卧、松解领扣、按合谷穴、心理护理、保暖、进饮食等处置。结果：91例药物不良反应，有89例顺利完成输液治疗，2例无效，更换药物。结论：有责任感，加强观察护理及时发现患者反应前驱症状，迅速采取有效措施，会取得满意效果。     

Biochemical and ultrastructural alterations produced by miconazole and econazole in Trypanosoma cruzi

Miconazole and econazole, two fungicide imidazole derivatives, completely inhibited growth of Trypanosoma cruzi (Tulahuen strain) at concentrations of about 20 muM. Culturing of T. cruzi in the presence of lower doses of imidazole derivatives produced: decrease of 5,7-diene sterol content in epimastigotes (including ergosterol); disappearance of the nuclear chromatin, vacuolization and decrease in the electron density of the cytoplasm; selective surface alterations as revealed by an increased response to wheat-germ- and phytohemagglutinin. At variance with the effect of miconazole on Candida (De Nollin et al. (1977) Antimicrobial. Agents Chemother. 11, 500-513), miconazole and econazole, under the experimental conditions used, did not increase the rate of hydrogen peroxide generation by T. cruzi.     

网状减张小切口缝合法提高大鼠背部张力皮瓣存活机制

Foropenseverewoundandthewoundafterdebridement,mostscholarsthinkthewoundshouldbeclosedindelayedfirst-phase.Meshrelaxingshortincision(MRSI)methodcanclosemoreskindeficiencyandhightensionopenwound,andavoidsubcutaneoushematomaandskinflapdrift.Intheexperiment,theexperimentalmodelonrattensionskinflaphasbeeninvolved,andthecontentofendothelin(ET)inratskinflaptissuehasbeenmeasuredinvariousperiodofwoundhealingaftermeshedrelaxingshortincisionsuturewithimmunohistochemistry,inordertodiscussprobablemechani…     

高效液相色谱法测定右旋儿茶素血浆浓度及药代动力学参数

本文建立了体液中右旋儿茶素的RP-HPLC测定方法。采用C_(18)键合相硅胶为填料的固相提取柱进行样品预处理,右旋儿茶素的提取回收率为79.8%.应用二极管阵列检测器对色谱峰纯度进行鉴定。该法精密度好,方法回收率近100%,日内、日间的变异系数为2.4～5.6%,血浓69.6～1160 ng/ml范围内呈线性关系,r=0.9993。家兔静注右旋儿茶素18mg/kg,其药代动力学过程符合二室模型,分布相半衰期为0.129 h,消除相半衰期为1.19h。     

傅立叶变换红外光谱用于不同状态胆囊组织的初步研究

目的:探索正常、炎性和癌变胆囊组织各自特异的傅立叶变换红外光谱(FT-IR)表现。方法:应用FT-IR对7例正常胆囊组织、7例炎性胆囊组织和2例胆囊癌组织标本进行检测,总结与组织状态相关的光谱特征。结果:胆囊正常、炎性和癌组织具有不同的FT-IR光谱表现,1550cm~(-1)处的酰Ⅱ带在癌组织中表现较弱,峰形低平,而在正常组织中则较强,峰形高尖。相叶强度Ⅰ1550与Ⅰ1647的比值在7例正常组织中为0.33,0.48.0.54,0.38,0.41,0.52,0.45,炎性组织中为0.32,0.27,0.34,0.25,0.33,0.28,0.29,2例癌组织分别为0.25和0.28;Ⅰ1080与Ⅰ1550的比值在正常组织中为0.059,0.042,0.077,0.085,0.092,0.100,0.081,炎性组织中为0.046,0.040,0.053,0.053,0.048,0.078,0.100,2例癌组织中为0.110和0.170。正常组织中1453cm~(-1)处的峰值高于1402cm~(-1)处,而在癌组织中则相反。结论:胆囊正常、炎性和癌组织的FT-IR表现不同,FT-IR有望成为胆道疾病临床诊断的一种新手段。     

Surgical relevance of diagnostic imaging techniques in pretherapeutic staging of carcinoma of the esophagus, stomach, colon and rectum

Summary. The increasing spectrum of therapeutic options for tumors of the gastrointestinal tract has resulted in a refinement of the pretherapeutic diagnostic strategies. The diagnostic approach in surgical institutions that are focused on primary surgical resection will therefore be much less sophisticated than in institutions who propose a selective therapeutic approach based on the pretherapeutic tumor stage and prognostic parameters. Pretherapeutic assessment of the depth of tumor infiltration, i. e. the T-category, is essential because most further diagnostic and therapeutic decisions are based on this information. This can today be achieved with a high degree of accuracy by endoscopy and endoscopic ultrasonography. Early T-stages (T1–2) are usually an indication for primary surgical resection and, after exclusion of distant metastases, no further diagnostic studies are required. In patients with locally advanced esophageal, gastric or rectum tumors (T3–4) multimodal therapeutic concepts should be considered. This usually requires additional diagnostic studies. None of the available diagnostic imaging modalities today allows satisfactory pretherapeutic assessment of lymph node metastases. The assumed nodular status should therefore currently not influence therapeutic decisions. Essential is, however, the assessment of distant metastases, since the documentation of distant tumor spread will change the therapeutic approach to a palliative situation. Detailed histologic and molecular-biologic assessment of tumor characteristics is growing in importance. This not only provides therapeutically relevant information regarding tumor grading, but opens the door towards a modern molecular diagnostic approach. It can be expected that in the near future a vast amount of relevant prognostic information can be obtained from endoscopic tumor biopsies, which may soon alter our therapeutic concepts.