Peptide map comparisons of similar serologically defined HLA-DR antigens isolated from different lymphoblastoid cell lines

In these studies, we have examined the possibility that DR subtypes, closely related to each other in structure, compose the major DR allotypic groups. The structures of DR molecules, isolated from pairs of cell lines that had the same serologically defined HLA-DR type, were compared by peptide mapping. HLA-DR molecules isolated from pairs of cell lines in which both members were either DR1, DR2, or DR7 were identical. However, DR molecules isolated from cell lines LG-29 (DR5) and LG-38 (DR5) displayed two distinct differences in their small (β) subunits. This number of differences, two of 15 peptides or 15%, is much fewer than is observed between DR allotypes (～50%) and suggests that at least two subtypes exist within the DR5 allotype family.     

血清性激素结合球蛋白与多囊卵巢综合征糖代谢的相关研究

目的:探讨血清性激素结合球蛋白(SHBG)与多囊卵巢综合征(PCOS)患者糖代谢的关系及干预治疗对其影响。方法:以16例正常育龄妇女为对照组,69例PCOS患者分为3组:非胰岛素抵抗(NIR)组、高胰岛素血症(HI)组、异常糖代谢(AMG)组,其中6例患者接受12周二甲双胍联合体育锻炼干预治疗,免放法测定血清SHBG水平。分别比较4组SHBG水平,分析SHBG与BMI、腰围、血压、Homa-IR、胰岛素敏感指数(ISI)等因素的相关性。结果:SHBG水平:HI、AMG>NIR>对照组;BMI、Homa-IR:AMG>HI>NIR组(P<0.01);ISI:AMG相似文献   

全身伽玛刀联合放化疗治疗肺癌所致上腔静脉综合征疗效观察

目的 探讨全身伽玛刀联合化疗治疗肺癌所致上腔静脉综合征(SVCS)的临床疗效。方法 本组31例SVC5均系经病理证实的肺癌病人，18例非小细胞肺癌先行全身伽玛刀治疗，再行化疗；13例小细胞肺癌先行化疗再行全身伽玛刀治疗(并辅以常规外照射治疗)，放疗结束后3—4周再巩固化疗1个疗程；同时配合消炎、利尿、抗凝等综合治疗。结果完全缓解(CR)12例，部分缓解(PR)16例，总有效率90．32％，中位缓解时间12．8个月。结论 综合治疗肺癌所致SVCS可提高临床疗效。     

大剂量丙种球蛋白联合强的松治疗儿童扩张型心肌病的护理

扩张型心肌病传统治疗效果欠佳,病死率高,预后差,是致死性心力衰竭的常见原因。对32例扩张型心肌病初发患儿,在传统治疗方法的基础上采用大剂量丙种球蛋白联合强的松治疗,取得明显疗效。而在护理上,应落实各项护理措施,加强心理护理、观察病情变化,加强消毒隔离预防感染,认真执行药物治疗过程中的操作规程,密切观察药物疗效及副作用,及时发现问题并予相应处理。     

GLA对肺癌细胞株E-CD表达及侵袭能力的影响

目的:观察GLA对肺癌细胞株体外生长抑制,E-CD表达及侵袭能力的影响。方法:采用细胞计数、SP免疫组织化学、图像分析及侵袭小室等方法,观察人肺腺癌细胞株A549、小细胞肺癌细胞株NC-H446在GLA、ARTA处理不同时间后细胞生长、E-CD表达及侵袭能力的影响。结果:在GLA干预下,NC-H446、A549两细胞株生长受到抑制,E-CD阳性表达率分别由原来的12%±8%、23%±11%提高到34%±9%、39%±13%,IOD分别由78.5%±16.9%、109.8%±13.2%增加到200.3%±21.7%、229.7%±11.0%,前后相比具有显著差异(P<0.01);穿过基质胶的细胞数分别为126.5±43.61、106.9±55.48,与对照组相比有显著差异(P<0.01);上述情况与ARTA相比,无明显差异(P>0.05)。结论:GLA能明显抑制两细胞株生长,促进E-CD的正常表达,减弱肿瘤细胞的侵袭转移能力。     

Cytomegalovirus immune globulin after liver transplantation: a cost-effectiveness analysis

OBJECTIVE: Cytomegalovirus (CMV) immune globulin (CMVIG) has been shown to significantly reduce severe CMV-associated disease complicating orthotopic liver transplant (OLT). We evaluated the economic impact of severe CMV-associated disease and calculated the marginal cost-effectiveness (C/E) of routine prophylaxis with CMVIG after OLT. DESIGN: C/E analysis. SETTING: Four teaching hospitals in Boston. PATIENTS: Patients who underwent OLT from January 1988 through June 1990. MEASUREMENTS: We gathered actual cost data of hospital care for patients enrolled in a clinical trial of CMVIG prophylaxis in OLT. We calculated average outpatient expenses from a separate group of patients undergoing OLT and developed a regression model to estimate costs during the first year post-transplant (R2 = 0.77). Based on this model, we calculated variable costs (in 1999 US dollars) for all patients in the randomized trial. From the published literature we obtained the probability of CMV outcomes and of long-term survival after OLT. We then developed a decision analytical model to determine an incremental C/E ratio, using a Markov simulation to estimate long-term survival and long-term costs. We discounted costs and life-years at 3% and 5% per yr. RESULTS: Based on the efficacy rate of 54% in the controlled trial, we estimate that CMVIG will increase life expectancy by 0.65 discounted years at an additional cost of $11,600, providing a marginal C/E ratio of $17,900/yr life saved. Examining the confidence limits of efficacy, we estimate that CMVIG will have a marginal C/E ratio of $66,200 gained/yr at an efficacy of 11% and $14,000 gained/yr at an efficacy of 83%. CONCLUSION: After OLT, prophylactic CMVIG has an incremental C/E ratio comparable to that of other well-accepted medical therapies and should be used routinely in these patients.     

Endocrine profiles of male workers with exposure to trichloroethylene

The objective of this study was to examine the endocrine profiles of a group of male workers chronically exposed to trichloroethylene (TCE) in an electronics factory. A total of 124 workers participated in a preliminary study, for which 85 satisfied the selection criteria and were recruited to take part in a more detailed study. Each of the 85 workers had urine collected and analyzed for trichloroacetic acids (TCA) on the day blood was taken for analysis of serum testosterone (T), follicle-stimulating hormone (FSH), dehydroepiandrosterone sulphate (DHEAS), and sex-hormone binding globulin (SHBG). Environmental TCE exposures were conducted for 12 workers. The geometric mean concentration of environmental TCE was 29.6 ppm (range 9–131) and the mean urine TCA was 22.4 mg/g creatinine (range 0.8–136.4). The results showed that years of exposure to TCE were significantly correlated with DHEAS and negatively correlated with SHBG and T levels. Serum FSH, T, and SHBG levels showed a gradual decline with increasing years of exposure to TCE. This dose-response decrease indicated that there was a disruption of peripheral endocrine function. This disruption could be a result of TCE-induced liver malfunction. The most dramatic change was that the increase in DHEAS concentration was associated with years of exposure to TCE, rising from 255 to 717.8 ng/ml for <3 to ≥7 years exposure, respectively. This evidence suggests that chronic exposure to TCE may also affect adrenal function. These findings, however, must be confirmed by further investigations. Am. J. Ind. Med. 32:217-222, 1997. © 1997 Wiley-Liss, Inc.     

Association of free androgen index and sex hormone–binding globulin and left ventricular hypertrophy in postmenopausal hypertensive women

The aim of the present study was to explore the relationship between androgen and LVH in postmenopausal hypertensive women. Enrolled in this study were 378 postmenopausal hypertensive women who were admitted to the department of cardiology between December 2018 and December 2020. According to left ventricular mass index (LVMI) evaluated by echocardiography, the patients were divided into LVH group (n = 172) and non‐LVH group (n = 206). Their clinical characteristics were collected. Based on the result of propensity score matching analysis, 160 cases in each group were matched successfully. After correcting for confounding factors by various models, the results showed that free androgen index (FAI) and sex hormone–binding globulin (SHBG) were the influencing factors of LVH in postmenopausal women with hypertension. Patients with elevated SHBG were 5% less likely to develop LVH than those without elevated SHBG (OR: 0.950, 95% CI 0.922‐1.578). Postmenopausal hypertensive patients with elevated FAI were 16% more likely to have LVH than those without elevated FAI (OR: 1.608, 95% CI 0.807‐3.202). Multiple linear regression showed that LVMI increased by 61.82g/m² for every 1 unit increase in FAI. In addition, SHBG decreased by 1 nmol/l, and LVMI increased by 0.177g/m². Subgroup analysis showed that patients in the controlled BP group had a lower risk of LVH for every additional unit of SHBG compared with the uncontrolled BP group. The risk of LVH for each additional unit of FAI in the uncontrolled BP group was higher than that in the controlled BP group. The results of this present study showed that the occurrence of LVH was positively correlated with FAI and negatively correlated with SHBG in postmenopausal women with hypertension. The increase in FAI level and the decrease in SHBG level may be related to the occurrence and development of LVH in postmenopausal hypertension.     

大剂量丙种球蛋白治疗葡萄球菌烫伤样皮肤综合征的临床观察

目的：探讨静脉注射丙种球蛋白对葡萄球菌烫伤样皮肤综合征的疗效。方法：回顾性分析2007年至2009年收治的葡萄球菌烫伤样皮肤综合征共49例,根据治疗方法不同分为两组,对照组20例给予抗生素治疗,治疗组29例在对照组基础上加用静脉丙种球蛋白1g／（kg·d）,用一次,对两组患儿体温正常时间、皮肤红斑及疱疹停止发展时间、皮肤疱疹干燥时间、住院天数进行分析比较。结果：治疗3d后判定疗效。治疗组治愈20例,显效7例,有效2例,无效0例,总有效率90％;对照组治愈1例,显效8例,有效9例,无效2例,总有效率43％。结论：大剂量丙种球蛋白静脉注射治疗葡萄球菌烫伤样皮肤综合征疗效显著,可快速改善症状及体征,并提高治疗效果。