肠道革兰氏阴性杆菌及孤菌快速鉴定的研究

目的：为寻求肠道G杆菌及弧菌快速鉴定的方法。方法：菌种经增菌及分离培养后，取菌落接种于综合生化培养基，并在综合生化培养基管口悬挂硫化氢和靛基质试纸条。同时以克氏双糖铁培养基作对照，置37℃培养18-24h。取综合生化管培养基，外加测试氧化酶，共获取11项生化指标。结果：对689株不同菌种与综合生化管培养基和常规双糖铁培养的测试结果，符合率为99．97％，(7577/7579)和99．79％(7563/7579)。结论：综合生化管是适合医疗卫生单位微生物实验室的一种快速检验方法。     

造血干细胞移植病人出血性膀胱炎的危险因素分析

[目的]了解造血干细胞移植病人出血性膀胱炎发生的危险因素。[方法]对29例造血干细胞移植病人出血性膀胱炎的危险因素进行统计学分析。[结果]出血性膀胱炎的发生率34％(10/29例),经Logistic回归分析发现出血性膀胱炎的危险因素是预处理方案(OR值0.058 0),马利兰/环磷酰胺预处理方案出血性膀胱炎发生率高。[结论]预处理方案对出血性膀胱炎的发生有重要影响。     

我院平均住院日状况的调查分析

目的：通过对医院平均住院日情况的简要分析，以探索缩短平均住院日的有效途径。方法：从统计室提取数据，与病案核对，计算相对指标。结果：我院的平均住院日呈下降趋势，但与卫生部颁布的标准还有差距，中医各科和康复科科别的平均住院日更长一些。结论：我院的平均住院日还有潜力可挖，术前住院日多数病种都较长，应在医疗质量和护理质量上狠下功夫，加强科室间的协作，降低平均住院日，减少无效住院日。     

Use of multivariate statistical methods to identify immunochemical cross‐reactants

Quantitative competition immunoassays with appropriate combinations of antibodies give consistent dose‐response patterns which may be used to identify and estimate amounts of cross‐reacting compounds. Previously reported methods of analyzing cross‐reaction patterns include multiple regression, principal components analysis and minimum estimates of variance (MEV). Four other techniques which are preferable in theory have been surveyed: discriminant analysis (DA), maximum likelihood estimates (MLE), classification and regression trees (CART), and computational neural networks (NN). MLE and simple back‐propagation neural networks can estimate the concentration, as well as the identity, of individual compounds. These four methods worked well with unfitted, unscaled data from monoclonal assays of triazines, phenylureas and avermectins. Immunoassays must be properly designed to provide adequate data for pattern recognition. Cross‐reactivity pattern analysis will make multi‐analyte, multi‐antibody immunoassays feasible for many applications in toxicology and hazard assessment.     

医学综合评价性问题的定性分析与定量灰关联分析研究

采用定性分析与定量灰关联分析相结合的方法处理医学综合评价性的问题和诊断性的问题。定义了诊断值，诊断灰度和信息覆盖率等医学新概念，实例应用说明，这种分析研究和于临床诊断时，诊断符合率高，误诊率低，而且克服了一般医学诊断模式只能定性分析，不能作定量分析的缺点。     

基于分数低阶矩的非高斯噪声中诱发电位提取新方法

诱发电位（EP）信号榆测与分析技术是临床医学诊断神经系统损伤及病变的重要手段之一。传统的EP信号提取与分离方法中，通常认为EP信号中混入的EEG等噪声是高斯分布的。近年来一些研究表明TEEG信号具有一定的非高斯特性。α-稳定分布町以更好地描述实际应用中所遇到的具有显著脉冲特性的EEG噪声。文中简要介绍了稳定分们统计特性，推导了一种适用于EP信号分离提取的新算法。计算机模拟和分析表明，这种算法是一种在分数低阶α稳定分布背景噪声条件下具有良好韧性的EP信号分离提取方法。     

Allele-specific PCR and Next-generation sequencing based genetic screening for Congenital Adrenal Hyperplasia in India

Genetic screening of Congenital Adrenal Hyperplasia (CAH) is known to be challenging due to the complexities in CYP21A2 genotyping and has not been the first-tier diagnostic tool in routine clinical practice. Also, with the advent of massive parallel sequencing technology, there is a need for investigating its utility in screening extended panel of genes implicated in CAH. In this study, we have established and utilized an Allele-Specific Polymerase Chain Reaction (ASPCR) based approach for screening eight common mutations in CYP21A2 gene followed by targeted Next Generation Sequencing (NGS) of CYP21A2, CYP11B1, CYP17A1, POR, and CYP19A1 genes in 72 clinically diagnosed CAH subjects from India. Through these investigations, 88.7% of the subjects with 21 hydroxylase deficiency were positive for eight CYP21A2 mutations with ASPCR. The targeted NGS assay was sensitive to pick up all the mutations identified by ASPCR. Utilizing NGS in subjects negative for ASPCR, five study subjects were homozygous positive for other CYP21A2 variants: one with a novel c.1274G>T, three with c.1451G>C and one with c.143A>G variant. One subject was compound heterozygous for c.955C>T and c.1042G>A variants identified using ASPCR and NGS. One subject suspected for a Simple Virilizing (SV) 21 hydroxylase deficiency was positive for a CYP19A1:c.1142A>T variant. CYP11B1 variants (c.1201-1G>A, c.1200+1del, c.412C>T, c.1024C>T, c.1012dup, c.623G>A) were identified in all six subjects suspected for 11 beta-hydroxylase deficiency. The overall mutation positivity was 97.2%. Our results suggest that ASPCR followed by targeted NGS is a cost-effective and comprehensive strategy for screening common CYP21A2 mutations and the CAH panel of genes in a clinical setting.     

Frequency domain models of the EEG

The structure of the normal resting EEG crosspectrum SVV(omega) is analyzed using complex multivariate statistics. Exploratory data analysis with Principal Component Analysis (PCA) is followed by hypothesis testing and computer simulations related to possible neural generators. The SVV(omega) of 211 normal individuals (ages 5 to 97) may be decomposed into two types of processes: the xi process with spatial isotropicity reflecting diffuse, correlated cortical generators with radial symmetry, and processes that seem to be generated by more spatially concentrated, correlated sources. The latter are reflected as spectral peaks such as the process. The eigenvectors of the xi process are the Spherical Harmonic Functions which explains the recurring pattern of maps characteristic of the spatial PCA of qEEG data. A new method for estimating sources in the frequency domain which fits dipoles to the whole crosspectrum is applied to explain the characteristics of the localized sources.     

呼吸功能不全综合实验改革的探索

目的:为了探索如何在一次教学实验中将生理学、病理生理学和药理学三个机能学科的实验有机地结合起来, 形成综合性实验以培养学生的综合思维和实验研究能力。方法:在病理生理学的呼吸功能不全实验的基础上, 增加了呼吸的生理调节和不同类型的呼吸衰竭采用不同的针对性药物治疗, 并且采用启发式教学方法。结果:学生学习兴趣明显提高, 学生不仅学到了更多的研究方法, 而且综合分析和科研创新能力都有明显提高。结论:这种改进明显提高了实验课的教学水平和教学质量。     

Segmentation of Bacteria Image Based on Level Set Method

In biology ferment engineering, accurate statistics of the quantity of bacteria is one of the most important subjects. In this paper, the quantity of bacteria which was observed traditionally manuauy can be detected automatically. Image acquisition and processing system is designed to accomplish image preprocessing, image segmentation and statistics of the quantity of bacteria. Segmentation of bacteria images is successfully realized by means of a region-based level set method and then the quantity of bacteria is computed precisely, which plays an important role in optimizing the growth conditions of bacteria.