胰岛素、胰岛素样生长因子Ⅰ及其受体在PCOS患者子宫内膜的表达

目的探讨胰岛素及其受体、胰岛素样生长因子I及其受体在多囊卵巢综合征患者子宫内膜的表达特点。方法利用免疫组化实验技术分析INS、INS-R、IGF-I和IGF-IR在多囊卵巢综合征和非多囊卵巢综合征不孕症患者子宫内膜中的表达情况,对结果进行图像分析,并利用SPSS10.0软件进行统计学分析,比较各组间的差异。结果1.多囊卵巢综合征患者子宫内膜IGF-I和INS-R表达水平明显高于对照组,差异有显著性(P<0.01)。其中多囊卵巢综合征中子宫内膜增生症患者的IGF-I和INS-R表达水平高于多囊卵巢综合征组增殖期患者,差异有显著性(P<0.05)。2.IGF-IR和INS在多囊卵巢综合征患者与对照组子宫内膜的表达水平无明显差异(P>0.05)。结论多囊卵巢综合征患者子宫内膜异常增生可能与局部IGF-I和INS-R表达异常有关。     

精神专科医院门诊抑郁症患者服药依从性影响因素分析

目的:探讨影响抑郁症患者服药依从性的影响因素.方法:选取2007年5月至10月于北京大学第六医院及北京回龙观医院门诊就诊的123例抑郁症患者,采用自编抑郁症患者就诊状况及服药依从性调查问卷进行调查,内容包括患者一般情况、患病及就诊情况调查、抑郁症发病次数、巩固治疗期坚持服药时间知晓情况及实际服药情况等.分别采用卡方检验及多元Logistic回归分析,探讨上述各因素与服药依从性的相关性.结果:完全依从组(完全按照医嘱时间和剂量服药的患者)和部分依从组(部分按照医嘱时间和剂量服药的患者)在医疗费用支付形式、病程、家属态度、抑郁症阳性家族史、对疾病性质的认识、就诊模式、第一主诉、患病次数、坚持服药时间知晓情况、家庭平均月收入等方面的差异有统计学意义(P＜0.05),将患者服药依从情况作为因变量,将以上项目作为自变量进行Logistic回归,结果显示月收入、对疾病性质的认识、家属态度、就诊模式、医药费支付形式、病程进入回归方程,各变量比值比分别为:10.932、9.604、7.246、6.019、2.611、0.209.结论:抑郁症患者服药依从性与多方面因素相关,对疾病性质的认识、家属态度、就诊模式、医药费支付形式、病程、家庭平均月收入等是影响抑郁症患者服药依从性的重要因素.     

Human growth hormone 1 (GH1) gene expression: complex haplotype-dependent influence of polymorphic variation in the proximal promoter and locus control region

The proximal promoter region of the human pituitary expressed growth hormone (GH1) gene is highly polymorphic, containing at least 15 single nucleotide polymorphisms (SNPs). This variation is manifest in 40 different haplotypes, the high diversity being explicable in terms of gene conversion, recurrent mutation, and selection. Functional analysis showed that 12 haplotypes were associated with a significantly reduced level of reporter gene expression whereas 10 haplotypes were associated with a significantly increased level. The former tend to be more prevalent in the general population than the latter (p<0.01), possibly as a consequence of selection. Although individual SNPs contributed to promoter strength in a highly interactive and non-additive fashion, haplotype partitioning was successful in identifying six SNPs as major determinants of GH1 gene expression. The prediction and functional testing of hitherto unobserved super-maximal and sub-minimal promoter haplotypes was then used to test the efficacy of the haplotype partitioning approach. Electrophoretic mobility shift assays demonstrated that five SNP sites exhibit allele-specific protein binding. An association was noted between adult height and the mean in vitro expression value corresponding to an individual's GH1 promoter haplotype combination (p=0.028) although only 3.3% of the variance of adult height was found to be explicable by reference to this parameter. Three additional SNPs, identified within sites I and II of the upstream locus control region (LCR), were ascribed to three distinct LCR haplotypes. A series of LCR-GH1 proximal promoter constructs were used to demonstrate that 1) the LCR enhanced proximal promoter activity by up to 2.8-fold depending upon proximal promoter haplotype, and that 2) the activity of a given proximal promoter haplotype was also differentially enhanced by different LCR haplotypes. The genetic basis of inter-individual differences in GH1 gene expression thus appears to be extremely complex.     

PLPP2: Potential therapeutic target of breast cancer in PLPP family

PLPPs (Phospholipid phosphatases) are widely expressed in different human tissues, regulate cell signal transduction, and are overexpressed in cancers such as gliomas, pancreatic adenocarcinoma, lung adenocarcinoma, and so on. As a member of the PLPP family, PLPP2 (phospholipid phosphatase 2) plays a vital role in the occurrence and development of breast cancer, but its mechanism is still unclear. Our research found that PLPP2 was overexpressed in breast cancer, and the higher expression level of PLPP2 showed a worse prognosis for breast cancer patients. Further analysis showed that overexpression of PLPP2 affected the expression of CDC34 (cell-division cycle 34), LSM7 (Like-Smith 7), and SGTA (small glutamine-rich tetratricopeptide repeat-containing protein alpha) through EMT (epigenetic-mesenchymal transition) related pathways to promote the occurrence and development of breast cancer. In vitro, silencing PLPP2 significantly reduced the proliferation, invasion, and migration abilities of human breast cancer cells MDA-MB-231. ER+ is a common subtype of breast cancer. Furthermore, we found that the overexpression of PLPP2 was significantly related to the poor prognosis of ER+ breast cancer. These results indicate that PLPP2 has value as a potential therapeutic target for breast cancer, especially for ER+ breast cancer.     

Kaposi’s sarcoma following malignant mesothelioma

We report the unusual occurrence of Kaposi’s sarcoma following asbestos-related malignant mesothelioma, in a human deficiency virus (HIV)-negative Italian man. Seropositivity to human herpes virus 8 (HHV8) was documented at the time of mesothelioma diagnosis and preceded the onset of Kaposi’ sarcoma with a time lapse of 13 months. HHV8 DNA was detected by polymerase chain reaction in lesional Kaposi’s sarcoma but not within mesothelioma. By immunostaining, mesothelioma cells expressed interleukin-6 and platelet-derived growth factor, which are important for survival of Kaposi’s sarcoma cells. Besides the possibility of a casual association, we hypothesize that mesothelioma-linked factors may have contributed to the development of Kaposi’sarcoma in the presence of HHV8 infection. Received: 12 April 1999 / Accepted: 24 June 1999     

巨噬细胞激活因子的分离纯化

由Ｔ细胞株（Ｍｏｌｔ ４）产生的巨噬细胞激活因子（ＭＡＦ）用阴离子交换和凝胶过滤色谱纯化。纯化的ＭＡＦ进行ＰＡＧＥ出现一条带，进行ＳＤＳ－ＰＡＧＥ证实其为单亚基，分子量为６００００；ＩＥＰ表明其等电点在ｐＨ３．５～４．５之间。     

类风湿性关节炎RF阳性与阴性患者外周血CD4+细胞基因表达的差异

目的:研究RF因子阳性及阴性RA患者之间是否存在基因组学的差异,探询差异存在的基因表达基础.方法:应用基因表达谱方法来检测上述两型患者CD4 淋巴细胞基因表达情况.结果:RF因子阳性与阴性患者之间有55条基因表达差异有统计学意义.结论:RF因子阳性与阴性RA患者之间存在差异表达基因,这些差异基因多与免疫应答相关.     

PBX1基因剪切体表达与SLE的相关研究

了解PBX1基因各种剪切体的表达在SLE患者和正常人中是否存在差异 ,探讨PBX1的表达与SLE发病的相关性。通过PCR扩增及毛细管芯片电泳 ,确证剪切体h、k、l存在于人体 ;通过实时荧光定量PCR技术 ,对剪切体h、k、l分别进行SLE患者组和正常组的mRNA表达定量比较。结果发现这 3种剪切体在患者组中的表达较正常人明显降低 ,正常人的表达是SLE的 9～ 12倍。重度患者的k、l剪切体与轻中度的病人相比表达明显降低 ,并发狼疮性肾炎的病人k剪切体的表达较无肾累及的病人显著降低。说明PBX1基因剪切体h、k、l在SLE患者中mRNA表达水平下降 ,并与SLE活动度及肾累及有关。提示机体通过PBX1的表达量的调节可能参与SLE的发病     

实验性自身免疫性甲状腺炎小鼠细胞免疫状态研究

作者检测了实验性自身免疫性甲状腺炎(EAT)小鼠的细胞免疫状态,结果表明:EAT小鼠脾细胞Thy-1.2和Lyt-2阳性T细胞数显著下降并伴随L3T4/Lyt-2阳性T细胞数比值升高;甲状腺球蛋白刺激的淋巴细胞增殖明显增强,但NK细胞活性无显著变化;脾细胞产生TNF和IL-1的水平明显高于正常小鼠。提示T细胞免疫功能紊乱和细胞因子的大量释放可能是引起此病的重要因素。     

综合医院内科与中医科门诊抑郁障碍患病率比较

目的调查沈阳市不同级别综合医院内科和中医科门诊病人抑郁障碍的患病率。方法采用分层随机整群抽样方法抽取沈阳市23家综合医院进行调查,共调查7448例年龄?15岁的内科和中医科门诊病人。调查员在门诊于病人就诊前使用扩展的一般健康问卷(GHQ-12)为筛查工具,将病人分为精神疾病高、中、低危险组,然后由精神科医生使用DSM-Ⅳ定式临床检查(SCID-I/P)对高、中、低危险组100%、40%、10%的调查对象进行SCID诊断。结果沈阳市综合医院内科门诊任何抑郁障碍的总现患率为11.01%(95%CI10.05%-12.06%),中医科抑郁障碍患病率为16.22%(95%CI13.23%-19.87%),高于内科门诊抑郁障碍患病率,RR值为0.68。结论沈阳市综合医院中医科门诊抑郁障碍患病率高于内科门诊,应该更加关注中医科就诊患者的抑郁障碍问题。