Cloning, distribution and functional analysis of the type III sodium channel from human brain

The type III voltage-gated sodium channel was cloned from human brain. The full-length cDNA has 89% identity with rat type III, and the predicted protein (1951 amino acids) has 55 differences. The expression pattern of human type III mRNA was determined in adult brain tissue and, in contrast to rat, was detected in many regions, including caudate nucleus, cerebellum, hippocampus and frontal lobe. The human type III channel was stably expressed in Chinese hamster ovary (CHO) cells and its biophysical properties compared to the human type II channel using identical conditions. The voltage dependence and kinetics of activation were found to be similar to that of type II. The kinetics of inactivation of the two human subtypes were also similar. However, type III channels inactivated at more hyperpolarized potentials and were slower to recover from inactivation than type II. When expressed in human embryonic kidney (HEK293T) cells, type III channels produced currents with a prominent persistent component, which were similar to those reported for rat type II [Ma et al. (1997) Neuron, 19, 443-452]. However, unlike type II, this was prominent even in the absence of coexpressed G-proteins, suggesting type III may adopt this gating mode more readily. The distinct properties of the channel, together with its wide distribution in adult brain, suggest that in humans, type III may have important physiological roles under normal, and perhaps also pathological conditions.     

多器官保存液的研究进展(Ⅰ)

本文综述了30年来多器官保存液的基础研究和临床应用的状况及进展。论述了器官保存的关键点。对现有的多器官保存液,包括:组氨酸-色氨酸-α-酮戊二酸溶液、威斯康星大学溶液、高渗枸橼酸盐腺嘌呤溶液、自制长征-1号溶液、Celsior溶液、Euro-Collins溶液、Carolina rinse溶液、St.ThomasⅡ溶液、乳果糖-组氨酸-聚蔗糖溶液、上海多器官溶液及其他器官保存液的成分及其特点进行分析,评估了上述器官保存液对脏器的保存效果,简述了中药在器官保存液中的应用。     

补肾复活汤治疗48例老年股骨颈骨折的疗效观察

目的:探讨补肾复活汤对老年股骨骨颈骨折患者的治疗效果.方法:用补肾复活汤内服治疗老年股骨颈骨折48例,设对照组48例,通过1～6个月的治疗,观察治疗后骨折的愈合情况.结果:治疗组患者折愈合率达64.6%.结论:补肾复活汤有明显改善骨折端的血液循环,促进骨折愈合的作用.     

中医药院校公共事业管理专业人才培养方案及课程体系科学化研究

为优化专业培养方案,使课程设置更加符合培养目标,采用文献分析、专家访谈、召开毕业生座谈会等方法,对中医药院校公共事业管理专业人才培养的总体思路、基本原则、培养目标和课程体系的科学化进行了研究,制定出医药经营管理、医疗保险、医院管理3个专业方向的本科学分制改革培养计划,并针对教学实践中存在的主要问题提出了相应的建议,为中医药院校公共事业管理专业的建设与发展提供参考.     

中医正骨学认知学徒模式与传统教学模式比较研究

目的探索适合模拟医院环境下的教学模式.方法选择中医系骨伤专业2000级学生为研究对象,分为实验组和对照组,分别采用认知学徒模式和传统教学模式教学,组织专家听课.课后对学生进行基础理论、临床技能测试和操作考核,并通过向学生及专家发放调查问卷听取专家和学生对认知学徒教学模式的评价.结果实验组学生的基础理论、临床技能测试、考核成绩均高于对照组,结果有统计学意义.调查问卷反映认知学徒模式具有营造合适的学习环境,激发学生的学习兴趣,加快学生对知识和技能的掌握速度的作用.结论认知学徒模式能最大限度发挥模拟医院在医学临床教学中的作用,是适合于模拟医院教学环境的教学模式.     

中医人才培养模式的思路与构想

中医人才的培养目标必须要适应社会需求,体现多层次多规格的特点,适应现代医学模式的转变,还要突出中医教育特色.中医人才的培养模式必须坚持多样化原则,主要培养学术型、临床型、传统型、中西医结合型、外向型、边缘型、其他等7类人才.     

本科中药专业就业情况用人单位调查分析

通过对医药企业用人单位的问卷调查,收集用人单位近年来接纳和拟接纳本科中药专业毕业生信息,以及对中药专业学生素质的要求,从而预测今后几年本科中药专业学生就业趋势.     

要重视中药的安全性研究

从中药的药品质量、用药剂量、用药时间、给药途径、药物相互作用、临床前研究等方面分析了影响中药安全性的原因,并提出了提高中药安全性的相应对策.     

中医药治疗血尿的临床体会

针对肾脏疾病血尿病程长、症情复杂、兼症多和易反复等特点，结合医案介绍了沈庆法教授运用中医药治疗血尿的临床经验和用药体会。     

感冒、水肿、便秘证治体会

结合多年的临床实践，总结了对感冒、水肿和便秘等中医内科疾病的独特认识，并附病案佐证。