小儿鼠药中毒血液透析的护理

目的 探讨鼠药中毒血液透析的疗效和护理经验。方法采用静滴同型全血，提高体内新鲜血液的含量，清除毒物。结果血液透析35例，共进行98例次，透析后有少数病例出现低血压、抽搐等不良反应。结论小儿灭鼠药中毒后，及时进行血液透析，是非常重要的。     

桃红四物汤对激素诱导骨髓间充质干细胞成脂分化的干预作用

背景：临床组织学及病理学研究证明：激素性股骨头缺血性坏死的早期病理变化是股骨头骨髓内造血组织减少,脂肪组织增多,这可能与激素诱导下骨髓间充质干细胞的成脂分化有关。目前被很多学者用来解释激素性股骨头缺血性坏死的发病机制。 目的：观察桃红四物汤对激素诱导骨髓间充质干细胞成脂分化的干预作用。 方法：体外培养大鼠骨髓间充质干细胞,通过大剂量激素诱导体外培养的骨髓间充质干细胞成脂分化,在诱导成脂的同时给予桃红四物汤含药血清干预。检测干预6 d后细胞内成脂标志物三酰甘油、PPARγ mRNA和aP2 mRNA的表达。 结果与结论：桃红四物汤含药血清可对抗激素诱导下的骨髓间充质干细胞三酰甘油、PPARγ mRNA和aP2 mRNA表达的增加。提示桃红四物汤防治激素性股骨头缺血坏死的机制不仅是改善股骨头的微循环,同时还与其抑制激素诱导下的骨髓间充质干细胞成脂分化有关。     

The effect of arsenic exposure and the efficacy of DMSA on the proteins and lipids of the gill tissues of Labeo rohita

Arsenic is a naturally occurring, highly toxic environmental pollutant. Fourier transform infrared (FT-IR) spectroscopy is a non-disturbing technique which provides quantitative information about the molecular composition of biological samples. The aim of this work is to study the compositional and structural changes at the molecular level occurring in gill tissues of Labeo rohita fingerlings due to arsenic exposure for various exposure periods by using FT-IR spectroscopic technique. The results of the present study suggest that arsenic exposure causes significant changes on the major biochemical constituents such as proteins, lipids and nucleic acids in the gill tissues of L. rohita. The changes are more pronounced as the period of exposure is increased. The significant decrease in the intensity and area of the amide I peak and CH₃ asymmetric stretching band suggests an alteration in the protein profile and lipid levels respectively, due to arsenic exposure. The amide A peak shifts suggests a change in the level of protein amide hydrogen bonding due to arsenic exposure. Further, the treatment with meso-2,3-dimercaptosuccinic acid (DMSA) improves the levels of biochemical constituents significantly, which suggest that DMSA treatment reduces the toxic effects and helps the recovery of gill tissues and its return to the level of the control.     

乙型肝炎患者免疫功能的检测及其临床意义

研究乙型肝炎患者外周血T淋巴细胞亚群、NK细胞和血清免疫球蛋白的变化及临床意义.采用流式细胞仪检测150例乙肝患者和30名健康者(对照组)外周血T淋巴细胞亚群(CD3 CD4 、CD3 CD8 )、NK细胞,免疫散射法检测血清免疫球蛋白(IgG、IgM、IgA)变化.结果表明各临床类型乙肝患者NK细胞降低,与对照组比较有显著性差异(P<0.01);慢性乙型肝炎组、慢性重型乙型肝炎组、肝硬化组外周血CD3 CD4 、CD3 CD8 T细胞均下降,其中慢性重型乙型肝炎组外周血CD3 CD8 与对照组比较有显著性差异(P<0.01),急性乙型肝炎组外周血T细胞亚群变化不明显(P>0.05);各临床类型乙型肝炎患者血清免疫球蛋白IgG、IgA随着病情的进展逐渐升高,与对照组比较有显著性差异(P<0.01).因此认为慢性乙肝患者存在细胞免疫和体液免疫功能紊乱,免疫功能的检测对乙肝的诊断、治疗及预后的判断有着一定临床意义.     

帕金森病患者NK细胞亚群及T淋巴细胞亚群变化的临床意义

目的:探讨帕金森病(PD)不同年龄、不同病期、伴随症状患者外周血NK细胞亚群及T淋巴细胞亚群的变化及其临床意义。方法:应用先进的流式细胞仪(FCM)直接免疫荧光染色法检测47例PD患者外周血NK细胞亚群及T淋巴细胞亚群,并与健康人组进行对照与相关分析。结果:PD组CD3+、CD4+、CD8+、CD4+/CD8+水平较对照组均明显降低(P<0.05),而CD16+56水平则较对照组明显增高(P<0.05)。高龄、病情重及伴痴呆和抑郁的PD患者CD3+、CD4+、CD8+、CD4+/CD8+水平降低更显著(P<0.05)。结论:PD发病过程中存在T细胞免疫功能低下及NK细胞免疫平衡失调,高龄、病情重及伴痴呆和抑郁的患者NK、T细胞免疫功能异常降低更明显,此为PD病理生理基础赋予新的内涵,亦为PD的免疫干预性治疗提供新的途径。     

动作学习进展阶段划分的实例研究

针对目前国内外对动作学习进展阶段划分的不一致性,本文引用文献资料进行对比分析;并以铅球教 学为例,试图进一步阐明:以动作表现的测量指标即量化来客观进行动作学习阶段的划分,以供铅球分层次 教学参考;为其他运动技术项目划分时相起到抛砖引玉的作用。     

射频热凝腰交感神经节联合中药治疗顽固性下肢灼痛36例

目的：观察射频热凝腰交感神经节联合温经止痛汤治疗顽固性下肢灼痛的临床疗效。方法：将72例患者随机分为观察组、对照组各36例。2组均给予射频热凝腰交感神经节治疗,观察组同时给予温经止痛汤,1剂/d,水煎3次,头两煎早晚分服,第3煎熏洗。结果：观察组完全缓解24例（66.7%）,部分缓解8例（22.2%）,未缓解4例（11.1%）,总有效率88.9%。对照组完全缓解12例（33.3%）,部分缓解10例（27.8%）,未缓解14例（38.9%）,总有效率61.1%。2组比较差异显著（P＜0.05）。不良反应发生率观察组为22.22%,对照组为25.00%,2组比较差异无统计学意义（P＞0.05）。结论：射频热凝腰交感神经节联合温经止痛汤治疗顽固性下肢灼痛临床疗效显著。     

Developing novel strategies to prevent parasitic infections

HIV is a devastating disease affecting millions of people worldwide despite the advent of successful antiretroviral therapy (ART). However, ART does not result in a cure and has to be taken for life. Accordingly, researchers are turning towards cure efforts, particularly in the light of two patients whose HIV has been seemingly eradicated. Numerous approaches and strategies have been considered for curing HIV, but no scalable and safe solution has yet been reached. With newly discovered difficulties in measuring the HIV reservoir, the main barrier to a cure, the only true test of cure is to stop ART and see whether the virus becomes detectable. However, it is possible that this treatment interruption may be associated with certain risks for patients. Here, we compare the current major approaches and recent advances for curing HIV, as well as discuss ways of evaluating HIV cure and the safety concerns involved.     

两种新型杀蟑剂对舰艇蟑螂的杀灭效果研究

目的：观察全无敌薰杀净和康星杀蟑胶饵对舰艇蟑螂的杀灭效果；方法：对厦门警备区73326部队的16艘舰艇进行现场灭蟑试验；结果：全无敌薰杀净处理的施药前蟑螂密度平均为388．11只／15min，处理后24h发现死蟑螂平均为l0645只／艘，平均密度下降率为99．6l％；康星杀蟑胶饵处理的艘舰艇施药前蟑螂密度平均为150只／min，处理后1d发现死蟑螂平均为2994只／艘，平均密度下降率为92.3％，9d后密度下降率为99．8％．2ld后来发现活蟑。结论：二种药物对舰艇上的蟑螂均见有很好的灭效。     

CLINICAL STUDY ON TREATMENT OF CERVICAL SPONDYLOTIC SYNDROME WITH BEETLE NEEDLE

The authors treated 68 cases of cervical spondylotic syndrome with beetle nee-dle,a new needle apparatus(made by themselves)which is not inserted into the skin of the humanbody.A total of 15 sessions of treatment were given to every patient.Results showed that 56 cases(82％)were cured,7 cases(10.3％)improved and 5 cases(7.4％)invalid.Just as its name implies,the beetle is instead of acupuncture needle.All the diseases being able to be treated with acupunctureneedle could also be treated with beetle needle.In addition,the beetle needle therapy has no violentpain,is safe,effective and easy to be popularized. It also has no harm to viscerae,no cross infection,no dangers of broken needle and of failing during treatment,so,the patient needs not to be in fear.The therapy is preferable for patients to accept.