自体皮与异体皮混合移植中的层粘蛋白表达

目的　通过对大张异体皮打洞嵌植小块自体皮混合移植皮片中层粘蛋白 (L N )的表达研究 ,探讨自体皮与异体皮混合移植促进 度烫伤创面愈合的机制。方法　清洁级 SD大鼠背部脱毛后 ,造成占体表总面积10 %～ 15 %的 度烫伤。伤后 3天切痂 ,移植大张打洞的异体皮。异体皮移植后第 3天 ,嵌植小块自体皮 ,完成皮肤混合移植。在异体皮移植后 3、5、7、14和 2 1天 ,采用免疫组织化学方法测定移植皮片中 L N的表达。结果　异体皮移植后第 7天出现排异反应 ,表现为异体表皮逐渐被爬行而来的自体表皮所铲除替代 ,异体真皮表面仍可见 L N阳性表达。异体皮移植后第 14天 ,从自体皮岛迁移来的表皮细胞下有零星 L N表达 ,异体皮中的 L N仍基本保持完整。异体皮移植后第 2 1天 ,自体表皮已基本覆盖异体皮区 ,L N表达已基本完整。结论　大张异体皮打洞嵌植小块自体皮混合移植中的异体皮为创面愈合提供了基底膜成分 ,有助于改善创面愈合的外观和功能     

四肢骨纤维异样增殖症的手术治疗

目的：总结病灶清除自体骨移植治疗四肢骨纤维异样增殖症的近期疗效。方法：1994年10月－2000年4月采用截骨、直视下病灶清除自体骨移植治疗13例（21处）骨纤维异样增殖症患者，结果：术后随访2－5年，1处复发，余患者植骨愈合，患肢功能正常，取肋骨后肋骨再生。结论：截骨病灶刮除植骨治疗纤维异样增殖症方法简单，复发率低，肋骨植骨，骨量大，易爬行替代，彻底清除病灶是防止复发的关键。     

Contact pressures at grafted cartilage lesions in the knee

The use of tissue-engineered cellular constructs is currently under clinical evaluation for the surgical treatment of articular cartilage lesions in the knee. The primary failure mode in such cartilage repair techniques is related to fixation. In addition, the repair tissue is believed to be very fragile in the post-operative period, and unable to support the intra-articular loads. We have developed a laboratory testing protocol in order to quantify the contact pressure distribution that develops on fibrin glue grafts applied to full-thickness cartilage lesions. The contact pressure distribution has been mapped on the contact surface of specimens subject to compression, in three configurations (intact, defect and grafted), at increasing load levels. All the maps show stress concentrations at the rim of the defect and a more uniform stress distribution around the rim after defect grafting. At a contact load of 180 N, the peak contact pressure measured on cartilage is 2.5 MPa. In presence of the graft, the peak pressures on the cartilage area surrounding the defect are reduced by 16%, on average. In contrast, both the mean contact pressure on the graft and the grafts contact area increase. The graft was found to carry around 80% of the total applied contact load, at all load levels tested. Fibrin glue was chosen as a grafting material in our study because it shows material properties very representative of currently-implanted cellular constructs. Thus, the results of this study have quantified aspects of recipient graft sites that may assist in optimising such grafting procedures from a biomechanical point of view.     

Conventional salvage chemotherapy vs. high-dose therapy with autografting for recurrent or refractory Hodgkin's disease patients

 Despite progress that has been made in curing Hodgkin's disease (HD), patients whose first remission is brief and those resistant to first-line chemotherapy still have a poor outcome. We retrospectively reviewed data from 29 patients with HD in first relapse or refractory to first-line chemotherapy. Following failure, all patients received three cycles of ifosfomide, epirubicin, and etoposide (IEV); moreover, 11 patients received a conditioning regimen followed by autografting. Of the 18 patients treated with IEV, eight (44%) are alive; nine died of disease progression, and one died of hematologic toxicity. The 24-month overall survival (OS), relapse-free survival (RFS), and event-free survival (EFS) are 18%, 44%, and 22%, respectively. Of the 11 patients treated with IEV and autografting, ten are alive (90%) and one patient died of progressive disease. The 29-month OS, RFS, and EFS are 91%, 71%, and 56%, respectively. Our results confirm data showing that patients with relapsed or resistant HD achieve a significantly better OS and EFS if treated with high-dose therapy and autografting. Received: January 28, 1999 / Accepted: July 21, 1999     

初次髋关节置换术中髋臼重建的策略

目的:探讨初次全髋关节置换术(THA)中髋臼重建方法的选择.方法:回顾性分析2001年至2006年间32例初次THA的患者(CroweⅢ、Ⅳ型先天性髋关节脱位15例,髋关节感染后融合6例,陈旧性髋臼骨折11例),其中内移髋关节旋转中心6例,结构性植骨11例,颗粒松质骨打压植骨15例;有4例应用钛网,4例重建钢板,3例加强杯固定.术后进行临床评估及X线评估.结果:术后平均随访41(8～72)个月,Harris评分平均为84分;3例臼杯松动需要进行翻修手术.结论:将髋臼重建在真臼位置,并进行软组织松解及下肢长度的平衡处理,通过不同的重建方法可以获得良好的髋关节功能.     

用自体游离骨膜治疗肿瘤性骨缺损

自１９８９年９月～１９９３年７月，用自体游离骨膜治疗肿瘤性骨缺损１２例。骨巨细胞瘤７例，孤立性骨囊肿２例，动脉瘤样骨囊肿２例，骨纤维结构不良１例。术前根据Ｘ线片测定瘤腔范围，以估计切取骨膜面积。术中先切取胜骨骨膜，剪成０．３ｃｍ×０．３ｃｍ小方块备用。而后彻底清除骨肿瘤病变组织，将备用骨膜植入瘤腔。１０周后拍Ｘ线片可见瘤腔内新生骨征象，１６周新骨密度已接近或相当于周围骨组织密度。     

自体骨块移植和Bio-Oss骨粉在上前牙区骨量不足种植术中的应用

目的探讨下颌第三磨牙区取骨用于上前牙区骨量不足种植术的方法。方法对于拟行上前牙种植,但骨量不足并有第三磨牙的患者,利用种植机截取合适的骨块,采用Bio-Oss和骨块移植,表面覆盖Bio-Gide胶原膜,同期或Ⅱ期植入种植体。结果所有种植体结合良好,6-8个月烤瓷牙修复,形态和功能良好,患者满意。结论上前牙牙槽骨不足可以运用此方法修复。     

不同植骨材料椎间融合效果的实验研究

目的观察不同植骨融合材料在兔腰椎椎体间脊柱融合的愈合情况,探讨不同的植骨材料替代自体骨用于腰椎椎体间融合的可能性。方法试验组分5组：空白对照组、自体骨组、同种异体骨组、生物陶瓷+自体骨组、重组人骨形态发生蛋白（rhBMP-2）复合体组。椎体间移植后4、8、12周,通过大体观察、手法检测、影像学、生物力学和组织学方法评价脊柱愈合情况。结果术后12周,大体及手法检测显示空白对照组不能自行达到脊柱融合,融合率为0％（0/12）;自体骨组脊柱融合率为83.3%（10/12）;同种异体骨组为66.7%（8/12）;生物陶瓷+自体骨组融合率为66.7%（8/12）;rhBMP-2复合体组为100%（12/12）,组织学显示成骨早,新骨形成量大,降解快,生物力学测试结果表明,自体骨组与同种异体骨组和生物陶瓷+自体骨组差异无统计学意义(P＞0.05),rhBMP-2复合体组优于其它组(P＜0.05)。结论同种异体骨、生物陶瓷+自体骨、rhBMP-2复合体均能替代自体骨行脊柱椎体间融合,rhBMP-2复合体更能促进脊柱愈合,提高脊柱愈合的生物力学强度。