Aseptic colon resection by an invagination technique

A new aseptic colon resection by an invagination technique is presented. The bowel to be resected is invaginated down into the healthy intestine, and the anastomosis is sutured in one layer of continuous suture before transection by a diathermy wire, placed in the intestinal lumen via the anus. Sections of bowel that cannot be invaginated,e.g.,because of a tumor, are first removed by transection between pairs of cable ties, which close the lumen. Twenty dogs were operated on without receiving prophylactic antibiotics. In 10, the intestine was transected between cable ties. An imprint, taken from the anastomosis and subcutis, was cultured. The bacterial count at the anastomosis exceeded 100 in only three cases; in the subcutis, this was the case in one dog. One wound infection developed. Serial barium enemas at 1, 2, 3, and 4 weeks revealed no anastomotic leakage. One early death because of a total anastomotic dehiscence was encountered, and two dogs were killed because of wound dehiscence and anastomotic stricture, respectively. It is concluded that, in dogs, the method is easily and safely performed, but further experimental studies are needed.This study was supported with grants from Fonden til Laegevidenskabens Fremme, Kraeftens Bekaempelse, Sygekassernes Helsefond, and Aarhus Universitets Forskningsfond.     

地塞米松鞘内注射与静脉注射在显微血管减压术后无菌性脑膜炎中的疗效比较

目的比较腰椎穿刺鞘内注射地塞米松与静脉推注地塞米松治疗显微血管减压术（MVD）术后无菌性脑膜炎（AM）的临床疗效。 方法选择自2015年1月至2020年1月于胜利油田中心医院神经外科就诊行MVD并诊断为AM的138例患者为研究对象,将患者分为对照组（68例）和观察组（70例）。对照组患者采用静脉推注地塞米松（10 mg/次）治疗,频率为按需给药;观察组患者采用腰穿放液联合鞘内注射地塞米松[60 μg/（kg·次）]治疗,频率为每日或隔日1次。比较2组患者治疗后的头痛及发热缓解情况、术后住院时间、治疗次数及激素不良反应情况。 结果治疗后8、72 h后,2组患者头痛、发热症状均明显好转,且观察组明显优于对照组,差异均有统计学意义（P<0.05）;观察组患者的术后住院时间[（7.68±2.23）d]短于对照组[（12.76±2.37）d],治疗次数[（3.5±0.6）次]明显低于对照组[（6.8±0.9）次],差异具有统计学意义（P<0.05）,2组患者均未见明显的激素不良反应。 结论腰穿放液联合鞘内注射地塞米松在治疗MVD术后AM患者中疗效确切,可有效改善患者头痛、发热等临床症状,减轻激素用量,缩短术后住院时间,具有重要的临床推广价值。     

Mumps hemiplegia

Neurological involvement of mumps is commonly restricted to aseptic meningitis. However, rarely mumps is associated with more severe encephalitic illness; other known associations described with mumps are cases of transverse myelitis and Gullain Barre like illness. We report a case of hemiplegia due to mumps parotitis probably caused by involvement of carotid artery.     

介入治疗股骨头无菌性坏死的疗效观察

目的：探讨介入治疗股骨头无菌性坏死的疗效。方法：采用经外周动脉穿刺插管的方法,经血管造影明确供血血管的位置,经导管向其内灌注扩张血管药、舒筋活血中药和溶栓药物,以改善股骨头供血状况;并追综随访1—3年,根据杨白明评定标准从疼痛、功能、X片3个方面进行判定,观察其临床症状的改善。结果：Ⅰ～Ⅲ期股骨头无菌性坏死病人的临床症状减轻,甚至消失;痊愈50％、显效25％、有效15％,（p〈O．05）。结论：通过对介入治疗股骨头无菌性坏死的疗效观察,发现其介入治疗Ⅰ～Ⅲ期股骨头无菌性坏死的疗效显著。     

双蒂髂骨瓣转位移植治疗股骨头无菌性坏死

目的:探讨双髂骨瓣转位移植在治疗股骨头无菌性坏死疾患中的应用效果。方法:我们自1998年5月～1999年2月对应用双蒂髂骨瓣移位移植治疗股骨头无菌性坏死的28侧32髋进行临床观察。结果:根据王岩等制定的评定标准进行评定:优10例,良14例,可2例,差2例。结论:双蒂髂骨瓣转位移植可改善股骨头的血运,促进坏死区的修复与重建.具有可靠的疗效。     

不同大小钛颗粒对骨髓间充质干细胞粘附能力的影响

目的研究磨屑钛颗粒对骨髓间充质干细胞（BMSCs）粘附能力影响的可能细胞分子机制。方法选用经体外传代纯化培养后的大鼠骨髓间充质干细胞（rBMSCs），与不同直径大小的钛颗粒悬液共孵育，再采用精准的流室系统对钛颗粒负荷的rBMSCs施加一定的流体剪切应力（FSS）后立刻固定细胞，经免疫荧光抗体染色，结合流式细胞术定量分析rBMSCs表面粘附分子表达的变化情况。同时设置相应的未经钛颗粒孵育的rBMSCs细胞为对照组细胞。结果剪切应力可明显上调rBMSCs表面粘附分子的表达。不同直径大小的钛颗粒可不同程度地抑制rBMScs的粘附能力，颗粒越小，抑制作用越明显。结论磨屑颗粒所引起的BMSCs细胞流变学异常是人工关节无菌性松动发生的可能机制。     

Factors influencing nurse compliance in maintaining aseptic technique in the insertion of peripheral intravenous access in neonates

BackgroundAseptic technique is the foundation for preventing bloodstream infections due to peripheral intravenous access insertion. This study aims at identifying the factors influencing nurse compliance in maintaining aseptic technique in the insertion of a peripheral intravenous catheter (PIVC).MethodologyThe research method is cross-sectional with 160 nurses. The related variables measured were repeated insertion, infant care (incubator or radiation warmer), age of nurse, working experience, level of education of nurses, level of knowledge, work schedule, and number of teams on duty. The nurse's knowledge was measured using a modified questionnaire, and the level of compliance was measured by an observation sheet.ResultsHigher age and education level of nurses were significantly associated with an increase in the level of nurse compliance (β = 0.053–1.22; p < 0.001; <0.015). Working experience, the number of insertions, the level of knowledge of nurses about PIVC, and the place of care in the radiant warmer were associated negatively with a decrease in the level of nurse compliance in the use of aseptic PIVC techniques (β = −0.011; - 0.152; p < 0.001).ConclusionAge and educational qualifications should be considered for the potential contributing to nurses' compliance with the aseptic protocol in the neonatal care unit.     

Lymphocytic choriomeningitis in Michigan

We summarize the first reported case of acquired lymphocytic choriomeningitis virus (LCMV) infection in Michigan to be investigated by public health authorities and provide evidence of the focal nature of LCMV infection in domestic rodents. Results of serologic and virologic testing in rodents contrasted, and negative serologic test results should be confirmed by tissue testing.