Pyruvate kinase M2 is highly correlated with the differentiation and the prognosis of esophageal squamous cell cancer

It's frequently stated that the pyruvate kinase M2 (PKM2) and Warburg effect are important for cancer development by accumulating more raw materials for macromolecule biosynthesis. However, the correlation between PKM2 and cancer is poorly reported. Here, we investigated the PKM2 expression in esophageal squamous cell cancer (ESCC). We observed that the expression of PKM2 was much higher in ESCC than in control normal tissue, and it is highly associated with many clinical features and prognosis. Specially, we found that the expression of PKM2 was closely related to the differentiation state of ESCC, and we further confirmed this discovery in vitro. As a result, out data indicated that PKM2 might be a useful indicator for determining the survival of patients with ESCC. Considering previous researches on the link among PKM2, Warburg effect, and differentiation, our study inferred the direct roles of PKM2 and Warburg effect in the differentiation of cancer cells rather than only providing synthetic intermediates for the promotion of cancer's progression.     

Alkaptonuria in the Trenčín District of Czechoslovakia

For several years the Clinical Genetics Research Laboratory at Martin, Czechoslovakia, has been studying alkaptonuria (AU) in the northern part of the District of Tren?ín in Slovakia. These affected individuals are part of a group of 103 alkaptonurics originated mostly in the mountainous parts of Slovakia. We report results of pedigree analyses; population and affected-family biochemical urine screening; estimation of inbreeding coefficient, of exogamy rate and of average marital distance and of calculation of the frequency of the AU allele, and of homozygotes and jeterozygotes in this portion of the Tren?ín District. Twelve homozygotes were found, but seven originated from a single hamlet in which a founder effect – genetic drift and inbreeding – are thought to account for the high prevalance of AU.     

舒血宁合并阿米替林治疗抑郁症多中心双盲对照研究

目的：观察合并应用银杏叶提取物能否提高经典抗抑郁药物阿米替林的抗抑郁疗效,能否减少阿米替林的不良反应。方法：采用多中心随机双盲对照研究方法。一组病人给予阿米替林合并银杏叶提取物（研究组）,一组给予阿米替林与安慰剂（对照组）,治疗８周。共纳入２４０例符合ＣＣＭＤ－２－Ｒ抑郁症标准的病人。结果：（１）研究组疗后Ｈａｍｉｌｔｏｎ抑郁量表平均减分率显著高于对照组;（２）研究组治疗结束时ＣＧＩ严重度显著低于对照组;（３）研究组Ａｓ－ｂｅｒｇ副作用量表治疗后增分显著低于对照组。（４）研究组的疗效指数显著高于对照组。结论：银杏叶提取物与阿米替林合并应用可以显著增强后者的抗抑郁疗效,并且能显著减少后者的不良反应,因而提高了后者的疗效指数。     

贫困大学生的成就动机与心理健康:社会支持的调节与中介作用

目的考察贫困大学生成就动机对其心理健康的影响,并同时考察社会支持在其中的调节与中介效应。方法采用成就动机量表、社会支持量表和心理健康症状自评量表,对358名滇西贫困大学生进行调查。结果成就动机与心理健康症状存在显著负相关(r=-0.448,P0.01),社会支持的调节作用不显著(t=0.141,P0.05),但社会支持的中介作用(β=-0.055,P0.01)显著;并且成就动机与心理健康的直接效应值(β=-0.353,P0.001)大于由社会支持中介的间接效应值(β=-0.055,P0.01)。结论以增强成就动机水平为主,提升社会支持程度为辅,可能是促进贫困大学生心理健康水平的有效方式。     

131I去除分化型甲状腺癌术后残余组织的疗效观察

目的 观察131I去除分化型甲状腺癌（DTC）术后残余组织的疗效.方法 采用131I去除甲状腺癌术后残余组织115例,131I剂量为2.96 ～ 3.7GBq（80 ～ 100 mCi）,服131 I后3个月停用甲状腺片,改服三碘甲状腺原氨酸片50μg/d,连续15天,再停药15天以上,做全身131I显像.结果 甲状腺癌术后残余组织去除率达70％.结论 131I去除甲状腺癌术后残余组织的疗效较好,可以有效防止甲状腺癌的复发和转移.     

Haploidentical Stem Cell Transplantation for Refractory/Relapsed Neuroblastoma

Pediatric patients with refractory or relapsed metastatic neuroblastoma (NBL) have a poor prognosis despite autologous stem cell transplantation (SCT). Allogeneic SCT from a haploidentical donor has a remarkable alloreactive effect in patients with leukemia; thus, we evaluated this approach in children with very high-risk NBL. We analyzed data from 2 prospective phase I/II trials. A total of 26 patients with refractory (n?=?5), metastatic relapsed (n?=?20), or locally relapsed MYCN-positive (n?=?1) NBL received a median of 17?×?10⁶/kg T/B cell-depleted CD34⁺ stem cells with 68?×?10³/kg residual T cells and 107?×?10⁶/kg natural killer cells. The conditioning regimen comprised melphalan, fludarabine, thiotepa, OKT3, and a short course of mycophenolate mofetil post-transplantation. Engraftment occurred in 96% of the patients. Event-free survival and overall survival at 5 years were 19% and 23%, respectively. No transplantation-related mortality was observed, and the single death was due to progression/subsequent relapse. The median duration of follow-up was 8.1 years. Patients in complete remission before SCT had a significantly better prognosis than those with residual tumor load (P?<?.01). All patients with progressive disease before SCT relapsed within 1 year. Grade II and grade III acute graft-versus-host disease (GVHD) occurred in 31% and 12% of the patients, respectively. Chronic limited and extensive GVHD occurred in 28% and 10%, respectively. Our data indicate that haploidentical SCT is a feasible treatment option that can induce long-term remission in some patients with NBL with tolerable side effects, and may enable the development of further post-transplantation therapeutic strategies based on the donor-derived immune system.     

Identification of C12orf4 as a gene for autosomal recessive intellectual disability

Intellectual disability (ID) is a major health problem in our society. Genetic causes of ID remain unknown because of its vast heterogeneity. Here we report two Finnish families and one Dutch family with affected individuals presenting with mild to moderate ID, neuropsychiatric symptoms and delayed speech development. By utilizing whole exome sequencing (WES), we identified a founder missense variant c.983T>C (p.Leu328Pro) in seven affected individuals from two Finnish consanguineous families and a deletion c.799_1034‐429delinsTTATGA (p.Gln267fs) in one affected individual from a consanguineous Dutch family in the C12orf4 gene on chromosome 12. Both the variants co‐segregated in the respective families as an autosomal recessive trait. Screening of the p.Leu328Pro variant showed enrichment in the North Eastern sub‐isolate of Finland among anonymous local blood donors with a carrier frequency of 1:53, similar to other disease mutations with a founder effect in that region. To date, only one Arab family with a three affected individuals with a frameshift insertion variant in C12orf4 has been reported. In summary, we expand and establish the clinical and mutational spectrum of C12orf4 variants. Our findings implicate C12orf4 as a causative gene for autosomal recessive ID.     

Coplanar Donor–Acceptor Semiconducting Copolymers to Achieve Better Conjugated Structures: Side‐Chain Engineering

It is reported on the allocation effects of branched alkyl chains, when used as solubility and ordering enhancers of the conjugated donor–accepter (D –A ) copolymer backbones, on the ordering and π–π overlapping of the copolymers, that drastically affect the electrical properties of organic field‐effect transistors (OFETs). Triisopropylsilylethynyl‐benzo[1,2‐b :4,5‐b ′]dithiophene (TIPSBDT) and diketopyrrolopyrrole (DPP)‐based copolymers, which have two linear alkyl spacers (methylene (C 1) or butylene (C 4)) between the DPP and side‐substituent (C₁₀H₂₁)CH(C₈H₁₇) , are synthesized by Suzuki cross‐coupling. These copolymer films are spun cast onto a polymer‐treated SiO₂ dielectric surface, and some are further thermally annealed. The longer spacer, C 4, is found to efficiently enhance the coplanarity and conjugation of the D –A backbone, while the C 1 does not. The resulting C 4‐bridged TIPSBDT‐DPP‐based copolymer readily develops a superior π‐extended layer on the dielectric surface; the edge‐on chains with randomly oriented side chains can be closely packed with a short π‐planar distance (d ₍₀₁₀₎) of 3.57 Å. Its properties are superior to those of the short spacer C 1 system with d ₍₀₁₀₎ ≈3.93 Å. The C 4‐bridged TIPSBDT‐DPP copolymer films yield a field‐effect mobility up to 1.2 cm² V⁻¹ s⁻¹ in OFETs, 12 times as higher than that of the C 1 spacer system.

     

He-Ne激光、微波、红外线和TDP灯照射对血粘度与红细胞影响的比较研究

目的比较研究He-Ne激光、微波、红外线和TDP灯照射对高血粘的影响,探索能否利用He-Ne激光、微波、红外线和TDP灯照射降低血液粘度,改善血循环.方法每份血样等分5份,1份作对照,对另外4份分别作He-Ne激光、微波、红外线和TDP灯照射处理.结果高血粘血样经He-Ne激光、微波、红外线和TDP灯照射作用后,全血表观粘度η降低(P＜0.01)、红细胞电泳率EPM加快(P＜0.01),血沉速度ESR提高(P＜0.01),一氧化氮、全血黏度、全血还原黏度降低(P＜0.01).红细胞变形指数降低(P＜0.01),红细胞聚集时间增加(P＜0.01),红细胞压积降低(P＜0.01),纤维蛋白降低(P＜0.01).结论He-Ne激光、微波、红外线和TDP灯照射能改善高血粘血样的流变特性.而且激光效果最好,依次是TDP灯、微波和红外线.进一步了解物理与临床治疗的密切关系.     

Training induced effects on blood volume,erythrocyte turnover and haemoglobin oxygen binding properties

Summary The effect of three weeks ergometer training (Tr) 5 times a week for 45 min at 70% by 6 subjects on erythrocyte turnover and haemoglobin O₂ affinity has been studied. Increased reticulocytosis could be observed from the second day after beginning Tr until a few days after its end, probably caused by increased erythropoietin release by the kidney. Erythrocyte destruction was most pronounced in the first and markedly reduced in the third week of Tr. Elevated glutamate oxalacetate transaminase activity and creatine as well as lowered mean corpuscular haemoglobin indicate a younger erythrocyte population in the first week of recovery. Total blood volume increased during the course of Tr by 700 ml, mainly caused by a raised plasma volume (74%). Red cell volume increased later with maximal values one week after Tr (+280 ml). In this week the standard oxygen dissociation curve was most shifted to the right (P₅₀ increased from 3.77±0.05 kPa to 3.99±0.07 kPa) and the Bohr coefficients had their lowest values. Both effects are completely explainable by the haemoglobin O₂ binding properties of young erythrocytes.After training, all parameters of physical performance ( , maximal workload, heart rate during rest and exercise) were markedly improved, indicating fast adaptation mechanisms. The increased erythrocyte turnover, including higher erythropoiesis, seems to be one important part of these effects.A preliminary report was presented at the National Congress of Sports Medicine in Cologne, 1982. Furthermore, the content of this paper was part of the doctoral thesis of the first author