Integration of municipal state,society and university efforts for sanitary risk prevention associated with Aedes aegypti mosquito in the metropolitan area of Buenos Aires,Argentina

The sanitary problem of Aedes aegypti mosquito acquires relevance around the world because it is the vector of dengue, zika, chikungunya and yellow fever. The vector is adapting to southern regions faster, and the propagation of these diseases in urban areas is a complex problem for society. We aimed to contribute to the risk prevention of disease transmission in the Metropolitan Area of Buenos Aires, through monitoring Aedes aegypti population levels and developing education campaigns with government agencies and society participation. Monitoring activities aimed to diagnose the presence of the vector and its ecology behaviour, and to generate education and prevention politics to avoid its propagation. The results show that (1) the mosquito is in the territory and it is spreading, (2) prevention activities of the municipalities are insufficient to generate an effective sanitary response and (3) it is necessary to improve the education programmes to the population about the life cycle of the vector. The integration of university, government and society improved the work of the team because it combined knowledge about vector ecology, diseases and territory characteristics.     

白纹伊蚊气味受体基因OR21α的克隆及定量分析

本文克隆白蚊伊蚊气味受体基因OR21a分析其是否与搜寻宿主或吸血行为相关。通过设计简并引物,PCR扩增得到白纹伊蚊气味受体基因OR21a段,采用SYBR荧光定量PCR方法,以自纹伊蚊β-肌动蛋白基因作为内参,比较了OR21a因在未吸血雌蚊、雄蚊、打断吸血雌蚊和饱血雌蚊头部表达量的差异。结果显示,白纹伊蚊OR21a因在未吸血雌蚊头部表达量最高,饱血雌蚊头部表达量最低,而在雄蚊头部的表达量介于打断吸血雌蚊和饱血雌蚊之间。本研究发现白蚊伊蚊会依据不同的生理需求改变OR21a因的表达量,推测可能与白纹伊蚊搜寻宿主及吸血行为有一定的相关性。     

上海市静安区白纹伊蚊对常用杀虫剂的抗药性调查

目的 了解静安区白纹伊蚊对常用杀虫剂的抗药性,为采取有效防控措施提供依据.方法 采用幼虫浸渍法、成蚊接触筒法测定白纹伊蚊对常用杀虫剂的抗性.结果 高效氯氰菊酯、倍硫磷、残杀威、溴氰菊酯、氯菊酯和双硫磷对静安区白纹伊蚊幼虫的LC50分别为0.012 5、0.005 3、1.467 9、0.007 8、0.0044和0.0...     

Diterpenes and their derivatives as promising agents against dengue virus and dengue vectors: A literature‐based review

Dengue, a mosquito‐borne viral disease, causes about 100 million cases of infection annually. It is a major public concern, and if left untreated or improperly diagnosed, may cause serious health problems or even death. Historically, dengue has not considered priorities for pharmaceutical companies made the available treatment options. Therefore, medicinal scientists are revealing new insights and enabling novel interventions and approaches to dengue prevention and control. Diterpenes, a class of terpenes have gained much attention due to their diverse biological effects. This review aimed at summarizing available evidences of diterpenes and their derivatives acting against dengue virus and their vectors. For this, an updated search was made in the databases: PubMed and ScienceDirect by using specific keywords. Among the 117 published reports, a total of 30 articles was included in this review. Findings suggest that a number of diterpenes and/or their derivatives act against dengue virus and their two potential vectors namely Aedes aegypti and Ae. albopictus. In conclusion, diterpenes and their derivatives may have the potential alternative therapeutic tools for the management of dengue virus and some associated diseases transmitted by Aedes mosquito.     

La reconstruction des enveloppes psychiques et environnementales dans les dessins d’enfants des rues en Haïti : une étude post-séisme

Objectives

The objective of this paper is to show how children and adolescents, living in the street in Haïti after the 2010 earthquake, use the street as a space to rebuild their identity. Using drawings of children and adolescents encountered in the context of research on resilience and the creative process among child and adolescent victims of the earthquake (ANR-10-HAIT-002 RECREAHVI), we explored the difficulties, the trauma and the creative expression of these children in the aftermath of the earthquake. These young people found themselves in a particularly vulnerable situation, whether socially, psychologically, or in terms of their safety. Their environmental envelopes were uncertain, fluctuating or damaged, so that the psychic envelopes of these street children was also put to the test. This article concerns the analysis of the drawings of street children and adolescents. We focus on the characteristics of the graphic envelopes to understand how these young street-dwellers symbolize the damage to their environmental and psychic envelopes, and how they express their needs for reconstruction.

不同地理种群白纹伊蚊线粒体基因COI的遗传多样性分析

目的探讨白纹伊蚊不同地理种群间的进化关系和遗传分化情况,为白纹伊蚊防制和蚊媒病防控提供基础资料。方法在广泛采集样品的基础上,通过PCR扩增、测序获取线粒体基因COI片段,并从GenBank下载了部分序列,比对、剪切后的598bp用于后续分析。结果系统发育分析的结果表明所有白纹伊蚊的COI序列聚成一支,没有明显的遗传差异;60条COI序列分属于19个单倍型,其中4个为共享单倍型;单倍型多样性(Hd)为0.737,核苷酸多样性(π)为0.20%;海南的白纹伊蚊种群与绝大部分地理种群间出现了明显的分化(P0.05);H1和H6形成了2个辐射中心,是较为原始的单倍型。结论我国的白纹伊蚊种群正处于扩张的趋势,海南的白纹伊蚊种群与其它地理种群间出现了明显分化。     

多房囊性肾细胞癌18例临床病理分析

目的：探讨多房囊性肾细胞癌（ mu1ti1ocu1ar cystic rena1 ce11 carcinoma,MCRCC）的临床病理学特征、诊断及鉴别诊断。方法回顾性分析18例MCRCC的临床病理学及免疫表型特征,并复习相关文献。结果18例中男性12例,女性6例,年龄26~68岁（平均55.6岁）。影像学检查均示多囊性占位,边界清楚。眼观：肿瘤切面见大小不等的多房囊腔,内含浆液性或血性液体。镜检：肿瘤囊内壁被覆单层透明细胞、复层上皮或上皮缺如,囊内成分大部分流失,纤维性囊壁或囊腔间隔中见灶状透明细胞,呈Ⅰ级细胞核,其为形态学诊断线索。免疫表型：透明细胞CD10、vimentin、EMA均阳性,Ki-67增殖指数低。18例患者平均随访43个月,均无复发或转移,预后良好。结论 MCRCC是一种罕见的肾细胞癌组织学亚型,预后良好,需与肾透明细胞癌囊性变及良性肾囊性病变相鉴别。     

苏云金芽孢杆菌杀蚊新菌株LLP29对白纹伊蚊作用机理的研究

从武夷山自然保护区白玉兰叶片上分离获得1株新的苏云金芽孢杆菌菌株LLP29,内含cyt1Aa6杀蚊基因。纯化的Cyt1Aa6毒素蛋白对白纹伊蚊幼虫和C6/36细胞都有高效活性。为更好地利用该菌株对白纹伊蚊进行生物防治,本试验以白纹伊蚊敏感品系及C6/36细胞为研究对象初步研究了其作用机理。免疫荧光染色和免疫组织化学实验结果表明:Cyt1Aa6毒素蛋白主要结合于C6/36细胞膜和白纹伊蚊幼虫中肠上。     

福建省首次从病人血清及蚊体分离出登革病毒的研究

目的　１９９９ 年入夏以来, 我省某地发生不明原因发热、头痛、肌痛、乏力、皮疹等症状的病人, 拟诊为登革热。为了从病原学上证实, 我们进行了病毒分离, 为防治工作提供科学依据。方法　应用早期病人血清７ 份, 白纹伊蚊１ 份经处理后接种Ｃ６／３６ 细胞, 并应用恢复期病人血清、双相血清、免疫血清及单克隆抗体进行免疫荧光法鉴定及分型。结果　在７份早期病人血清中经２ ～３ 代分离出５ 株登革病毒, 并从白纹伊蚊体内分离出１ 株登革病毒。结论　此分离的毒株经鉴定均为登革ＩＩ型病毒。此系福建省首次从病人血清及蚊媒体内分离出病毒的报告。不仅为防制工作提供科学依据, 而且为今后的研究奠定了基础。