地中海贫血孕妇ALP、AFU和甲状腺激素变化情况

目的探讨地中海贫血孕妇早期体内碱性磷酸酶(ALP)、α-L-岩藻糖苷酶(AFU)和甲状腺激素变化情况。方法选取2016年2月-2017年3月在该院产科建档并进行产检的地中海贫血早期孕妇281例,同时设置281例正常孕周为3个月孕妇作为对照组。采集空腹静脉血2 ml注入紫色EDTA-K2抗凝管中,完成血液检测;采集空腹静脉血4 ml,静止,分离血清,检测ALP、AFU、血清游离三碘甲状腺原氨酸(FT_3)、血清游离甲状腺激素(FT_4)和促甲状腺激素(TSH),PCR+反向斑点杂交法检测确认地中海贫血。结果对照组孕妇ALP和AFU水平高于地中海贫血孕妇(P<0.05);地中海贫血孕妇TSH高于对照组孕妇(P<0.05);地中海贫血孕妇FT3和FT4低于对照组孕妇(P<0.05)。结论地中海贫血孕妇早期ALP、AFU和甲状腺激素水平受到影响。     

儿童锌缺乏敏感指标的评价

为评价反映儿童锌缺乏的敏感指标.方法 我们对300名3～6岁儿童进行膳食调查与体格检查,同时测定了锌缺乏组和对照组儿童的尿锌与尿肌酐比值,血清ALP活性,血清胆固醇水平及锌缺乏儿童补锌治疗前后上述指标的变化.结果 锌缺乏组与对照组尿锌与尿肌酐比值,血清ALP活性有显著性差异.锌缺乏组儿童血浆锌,尿锌与尿肌酐比值,血清ALP活性,血清胆固醇水平在补锌治疗前后有显著性差异.结论 血浆锌和血清ALP同时降低可以预示儿童体内锌缺乏,需要补锌治疗;尿锌与尿肌酐比值能较敏感地反映儿童的锌营养状况;胆固醇与锌营养之间的关系值得进一步研究.     

失重下骨碎补总黄酮抑制JNK通路促间充质干细胞增殖的研究

目的:观察模拟失重状态下,骨碎补总黄酮抑制JNK通路促间充质干细胞增殖的作用机制。方法:体外培养大鼠骨髓间充质干细胞,制备含有骨碎补总黄酮药物的血清及不含药物的血清。随机分组给药,分为失重组、失重骨碎补总黄酮药物干预组(失重药物组)和失重空白血清组3组,随后建立失重模型,加入骨碎补血清培养液,观察形态学变化,噻唑蓝检测间充质干细胞增殖情况,PNPP检测碱性磷酸酶(alkaline phosphatase,ALP)表达,PCR测定氨基末端激酶(Jun Nterminal kinase,JNK)表达。结果:与失重组比较,失重空白血清组间充质干细胞增殖明显(P0.05),ALP活性明显升高,且差异具有统计学意义(P0.05)。失重药物组与其他组比较,JNK通路表达降低,差异具有统计学意义(P0.05)。结论:骨碎补总黄酮可能是通过抑制JNK通路促进失重下间充质干细胞增殖。     

肝型碱性磷酸酶N—糖链亲和层析用于原发性肝癌诊断研究

目的 研究肝型碱性磷酸酶Ｎ－糖链亲和层析在原发性肝癌诊断中的应用价值。方法 用麦胚凝集素ＷＧＡ分离血清中肝型碱性磷酸酶和骨型碱性磷酸酶,再将肝型碱性磷酸酶进行蔓陀萝凝集素ＤＳＡ亲和层析,分离不同亲和力组分,测定酶活力;将血清直接上样ＤＳＡ亲和层析柱,分离肝型碱性磷酸酶强结合组分。     

染色体核型及血清碱性磷酸酶水平在慢性粒细胞白血病病情预测中作用

目的 研究慢性粒细胞白血病(CML)患者骨髓单个核细胞染色体核型与血清碱性磷酸酶(ALP)水平间变化关系,探讨细胞遗传学变化及ALP水平在患者病情及预后判断中的价值.方法 选择不同时期CML患者,检测其血清ALP,并取骨髓有核细胞采用直接法或24小时培养法、R带显色技术作染色体分析.结果 CML患者ALP水平高于正常对照组,加速期急变期患者复杂核型出现率及血清ALP水平显著高于慢性期患者,且ALP水平与髓内幼稚细胞数水平呈正相关,PH1阳性核型CML患者与复杂核型CML患者血清ALP水平差别不明显.结论 CML患者血清ALP水平及染色体复杂核型反应该期患者疾病特征,对CML疾病分期、预后判断有价值,两者间的一致性有待进一步研究.     

可注射型热触发仿天然骨材料的基础研究

目的：试图研制可注射型热敏仿天然骨材料,测定其理化特性,并将其初步应用于体外根尖孔破坏的离体牙模型上。方法：采用逆向蒸发法制备载碱性磷酸酶（alkalinephosphatase,ALP）的脂质体,考察其包封率、活性率及相变温度。将载ALP、载钙离子（Ca^24）和磷酸盐（Pi）3种脂质体与β-甘油磷酸盐（β-glycerophosphate,β-GP）混合制备矿化液,观测矿物质的形态、元素分析及红外光谱曲线;将I型胶原与以上矿化液混合制备的复合材料应用于根尖孔破坏的离体牙根尖周围,根管充填并统计分析。结果：载ALP的脂质体平均包封率为25．1％,平均活性率为16．5％,相变温度为37．3～40．1℃,峰值为39．2℃;与未加ALP者相比,所得矿化物形态和Ca／Pi比与天然骨中矿化物更接近,红外光谱曲线表明其为羟基磷灰石;体外模型显示该复合材料可在一定程度上支托根充材料,预防超充。     

Current understandings and perspectives on non-cancer health effects of benzene: A global concern

Objective

Benzene, as a volatile organic compound, is known as one of the main air pollutants in the environment. The aim of this review is to summarize all available evidences on non-cancerous health effects of benzene providing an overview of possible association of exposure to benzene with human chronic diseases, specially, in those regions of the world where benzene concentration is being poorly monitored.

Methodology

A bibliographic search of scientific databases including PubMed, Google Scholar, and Scirus was conducted with key words of “benzene toxic health effects”, “environmental volatile organic compounds”, “diabetes mellitus and environmental pollutants”, “breast cancer and environmental pollution”, “prevalence of lung cancer”, and “diabetes prevalence”. More than 300 peer reviewed papers were examined. Experimental and epidemiologic studies reporting health effects of benzene and volatile organic compounds were included in the study.

Results

Epidemiologic and experimental studies suggest that benzene exposure can lead to numerous non-cancerous health effects associated with functional aberration of vital systems in the body like reproductive, immune, nervous, endocrine, cardiovascular, and respiratory.

Conclusion

Chronic diseases have become a health burden of global dimension with special emphasis in regions with poor monitoring over contents of benzene in petrochemicals. Benzene is a well known carcinogen of blood and its components, but the concern of benzene exposure is more than carcinogenicity of blood components and should be evaluated in both epidemiologic and experimental studies. Aspect of interactions and mechanism of toxicity in relation to human general health problems especially endocrine disturbances with particular reference to diabetes, breast and lung cancers should be followed up.