乳腺癌组织中—p—MAPK活化对c—fos和c—jun的激活作用

OBJECTIVE: This paper was to investigate the relationship between expression of p-MAPK and oncogenesis of breast cancer. METHODS: Immunohistochemical technique was used to detect the expression of p-MAPK and c-fos and c-jun proteins in 68 cases of breast cancers, 42 cases of pericarcinomatous tissues and 7 cases of normal breast tissues. RESULTS: Positive stainings of p-MAPK, c-fos, and c-jun were localized in cancer cell nuclei. The positive rates of p-MAPK, c-fos, and c-jun were 86.8% (59/68), 82.4% (56/68), and 77.9% (53/68), respectively, which were much higher than that in pericarcinomatous tissues (P < 0.01). Of some cases, p-MAPK positive staining was also found in the nuclei of the fibroblastic and angioendothelial cells of cancer stroma. CONCLUSION: Activated or overexpressive MAPK activates the immediately-early oncogenes(c-fos, c-jun), which might play an important role in carcinogenesis of breast cancer and be an early event of oncogenesis of breast cancer.     

A／B超对脉络膜恶性黑色素瘤的诊断价值

目的：探讨A／B超对脉络膜黑色素瘤的诊断价值。方法：采用法国BVI公司超功能A／B超诊断仪,观察分析了38例脉络膜黑色素瘤超声显像的特点。结果：经确诊的脉络膜恶性黑色素瘤在B超图像上具有特征性改变,即脉络膜黑色素瘤变性隆起,穿破脉络膜的玻璃膜呈圆形,半球形,蘑菇状等实质性光团及弥散性扁平状球壁回声增厚,肿瘤内有挖空现象,近半数有脉络膜凹,部分病例有声影,可继发视网膜脱离及青光眼。结论：A／B超对诊断脉络膜恶性黑色素瘤具有特异性诊断价值。     

预真空灭菌器B-D试验失败的原因与处理方法

Ｂ-Ｄ试验全称叫布维 狄克试验 (Ｂｏｗｅｎ Ｄｉｃｋｔｅｓｔ) ,是专用于预真空压力蒸汽灭菌器空气排除效果的检测方法[1 ] 。它是灭菌前或检修完毕后必须进行的灭菌器空锅灭菌测试工作。Ｂ Ｄ试验失败表现在指示图上的异常颜色变化有以下 4种情况 :①四周为标准的褐色或黑色 ,中间部分有 4～ 1 0ｃｍ2 明显未变色。②中间部位变色比外部淡 ,呈由外到内逐渐变淡的现象。③变色不均匀 ,也无规律。④褐色或黑色的条纹上呈现条状或块状白色斑块 ,边缘较模糊。现就Ｂ Ｄ试验失败的原因及处理方法分析如下。1　材料和方法　　山东新华医疗器械厂…     

B超在腹腔镜胆囊切除术中的应用价值

目的：探讨B超在腹腔镜胆囊切除术中的应用价值。方法：本文对526例术前24h及手术的详尽资料进行分析。结果：术前B超检查,可以地腹腔镜胆囊切除术手术适应证的选择提供依据。并对手术的难易程度作出估计。结论：术前B超检查在腹腔镜胆囊切除术中有重要的价值。     

荧光定量—PCR法检测柯萨基病毒B组及临床应用

目的：探讨对柯萨基病毒B组（Cox-VB)进行早期诊断及其与临床疾病的关系。方法：用荧光定量（FQ）-PCR法对疑似Cox-VB感染的患儿进行Cox-VB-RNA定量检测。结果：110例疑似Cox-VB感染的患儿,阳性44例,阳性率为40.0%。结论：FQ-PCR法可快速、准确、定量地检出标本中的Cox-VB。     

B超检查对腹腔镜胆囊切除术的作用分析

对540例胆囊结石病人彩和B超术前检查以预测手术的难易度,并与手术结果对比,结果表明术前用B超检查预测手术难易度对于正确掌握手术适应症,增加LC的成功率,减少并发症有一定的意义。     

RELATIONSHIP BETWEEN HUMAN PARVOVIRUS B19 INFECTION AND APLASTIC ANEMIA

Objective. To explore the relationship between human parvovirus B19 (HPV B19) infection and aplastic anemia (AA) and to investigate the role of HPV B19 in the occurrence of AA. Methods. The presence of HPV B19 DNA was detected in the peripheral blood samples of 60 patients with AA (children 38 and adults 22) by nested polymerase chain reaction (PCR) assay, and 30 healthy persons were selected as control. Results. Sixteen (26. 7 % ) of 60 AA cases were HPV B19 DNA positive, while all the samples in the control group were negative for HPV B19 (P = 0. 000914). Among the case group, the positive rates of HPV B19 DNA were21.4% (6 /28), 30.0% (3 / 10), 20.0% (1 /5) and 35.3% (6 / 17) in children acute AA (AAA), children chronic AA (CAA), adults AAA and adults CAA patients respectively, which were significant-ly higher than that in the control group, Furthermore, there was no remarkable difference between children AA and adults AA in the 16 HPV B19 DNA positive patients; neither was there between AAA and CAA. Conclusions. HPV B19 infection is not only correlated with the occurrence of children AAA and CAA, but also with adults AAA and CAA, and might be an important viral cause for AA in humans.     

维生素K1,维生素B12用于硬膜外腔术后止痛的临床观察

我院 1998年 1月～ 1999年 4月应用维生素 K1 、维生素B1 2 和胃复胺注入硬膜外腔进行术后止痛 ,现将临床观察结果报告如下。1　资料与方法1.1　一般资料　选择 ASA ～ 级无心肺功能异常的择期手术 75例 ,一般情况及分组见表 1。表 1　患者一般资料及分组组别性　别男女n平均年龄(岁 )上腹部手术n(% )下腹部及盆腔手术n(% )骨科手术n(% ) 组 6 1 939.96 3(1 2 ) 2 1 (84) 1 (4) 组 4 2 1 36.52 8(32 ) 1 6(64) 1 (4) 组 71 846.1 6 6(2 4 ) 1 5(60 ) 4 (1 6)1.2　方法　将所有病例随机分为三组 ,每组 2 5例。 组用维生素 K1 10 mg加入胃…     

80例强直性脊柱炎临床X线分析

目的：分析和研究强直性脊柱炎的临床Ｘ线检查诊断方法,Ｘ线分级、分期的表现及在诊断中的重要性,方法：对８０例强直性脊柱炎患者进行Ｘ摄片分级,分期,结合血沉（ＥＳＲ）,Ｃ－反应蛋白（ＣＲＰ）和ＨＬＡ－Ｂ２检查诊断。并进行统计学分析。结果：９０％患者ＨＬＡ－Ｂ２阳性,６０％血沉增快,骶髂关节和髋关节片以２级改变为主,腰椎病变以早、中期Ｘ线病变者较多。结论：强直性脊柱炎以青壮年男性为多见,ＨＬＡ－Ｂ２阳性     

肝脏海绵状血管瘤B超声像与CT扫描58例对照分析

目的 探讨B超扫描与CT扫描诊断肝脏海绵状血管瘤的意义。方法 58例病人同时接受B超扫描和CT增强扫描,对其声像图进行分析对比。结果 B超发现强回声型40例,低回声型10例,混合回声型8例,其诊断血管瘤的敏感性为100％(58／58),特异性为96．6％(56／58);CT扫描敏感性为94．8％(55／58),特异性为100％(55／55)。结论 B超诊断血管瘤敏感性高,特异性稍差,价格便宜。适于普查;CT则特异性高,适于最后确诊。两相结合,有很强的互补性。