bFGF-Math1基因诱导UEC-4细胞产生毛细胞的实验研究

目的进一步探讨bFGF-Math1基因在前庭上皮细胞系(UEC-4)细胞中的表达及生物学作用.方法利用阳离子脂质体LipofectamineTM2000介导bFGF-Math1基因真核表达质粒(pCI-bFGF-Math1)转染UEC-4细胞,利用RT-PCR技术检测基因在细胞中的表达,并利用免疫组织化学方法观察细胞的分化及特异性抗体的表达.结果脂质体介导的基因可在细胞内获得良好的表达,转染后24h,相差显微镜下观察细胞形态发生变化,免疫组织化学显示转染后细胞可以表达毛细胞特异性蛋白Myosin7a.结论bFGF-Math1基因有良好的生物学特性,可以有效地诱导支持细胞向毛细胞样细胞转变.     

Adenoviruses 16 and CV23 Efficiently Transduce Human Low-passage Brain Tumor and Cancer Stem Cells

Most clinical protocols involving adenovirus （Ad） vectors for gene therapy use a vector based on serotype 5 （Ad5）. We believe that this serotype is not suitable for all gene therapy applications and that alternative vectors based on other serotypes should be developed. We have compared the ability of Ad5, Adllp, Ad16p, and a chimpanzee Ad （CV23） to infect human low-passage brain tumor cells as well as primary glioma cells sorted into a CD133（＋） and CD133（-） population. Cancer stem cells have been shown to reside in the CD133（＋） population of cells in human glioma tumors and they are of considerable interest in glioma therapy.[第一段]     

反义P^53基因对人肺癌细胞生长蛋白表达的影响

Ｐ＾５３基因的突变浊肿瘤发生的重要事件之一，利用能表达反义Ｐ＾５３基因的逆转录病毒载体导入人肺癌细胞系ＧＬＣ－８２细胞，抑制其突变的Ｐ＾５３基因表达。并与导入的空载体ＰＤＯＲ－ｎｅｏ和未导入的ＧＬＣ－８２细胞作比较，用ＤＮＡ杂交证实重组子转入细胞，通过细胞生长曲线观察细胞生长情况，利用免疫组化法和流式细胞仪测定观察Ｐ＾５３蛋白表达，结果表明，反义Ｐ＾５３基因对ＧＬＣ－８２细胞生长速度及突变的Ｐ＾５     

携带HuIL—3基因的人肝癌特异性逆转录病毒转体的构建

构建携带人白细胞介素ｃＤＮＡ的人肝癌特异性闻生转录病毒载体。将ＨｕＩＬ－３ｃＤＮＡ克隆到逆转录病毒载体ｐＭＮＳＭ的ＰＬ位点，构建ＳＶ４０早期启动子驱动的载体ｐＭＮＳ－ＩＬ－３；从质粒ｐＧＥＭ，７Ｚｆ－ＡＦＰｅ中游离人甲胎蛋白增强子核心序列，先克隆 到ｐＭＮＳＭ的ＰＬ位点上，     

转B7基因在泌尿系肿瘤治疗中的应用前景

转Ｂ７基因在泌尿系肿瘤治疗中的应用前景邢念增俞莉章郭应禄作者单位：１０００３４北京医科大学第一医院，泌尿外科研究所人类的基因治疗是指将目的基因导入人体细胞，以达到治疗疾病的方法［１］。近十年随着ＤＮＡ重组技术的发展与对疾病分子生物学机制认识的深入，使...     

谈临床诊断治疗实验管理

医学是生命的科学,必然要求真,同时也包含着对伦理的追求。由于医学的对象是人,因此就显得格外突出,且内涵丰富。近年来由于基因治疗在的各种高科技医术的出现,人体实验问题引起了普遍关注。一、人体实验的现状人体实验是以人作为受试对象,用人为的手段有控制地对受试者进行观察和研究的医学行为过程。人体实验有治疗性实验和非治疗性实验如诊断性实验;另外根据实验者和受试者是否为同一个人,可分为自体实验和非自体实验。后者又根据受试者知情同意原则来划分为自愿性人体实验、强迫性人体实验和欺骗性人体实验。上述种种人体实验,除了自体…     

浅谈基因治疗的现状及其面临的困境

基因治疗将在未来的疾病治疗中扮演重要的角色，然而这一新颖的技术却面临着诸多问题，本文论述了基因治疗的发展现状及其所面临的技术难题。     

神经生长因子基因转染联合强化铁营养防治豚鼠爆震性聋的实验研究

Objective To study the protective effects of adenovirus-mediated human beta-nerve growth factor gene (hNGFβ) transfer combined with iron fortified nutrition on blast hearing damage in guinea pigs. Methods Deafness was induced by blast (172dB SPL) in 35 healthy guinea pigs. Seven days after noise exposure, 10 guinea pigs were inoculated with adenovirus-mediated hNGFβ (Ad-hNGFβ) into the perilymphatic space (the gene group), another 10 guinea pigs were given hNGFβ combined with iron fortified nutritional diet (the combination group), still another 10 guinea pigs were inoculated with artificial perilymphatic fluid (APF), and 5 guinea pigs served as control, given neither medicine nor noise exposure. Auditory brainstem response (ABR) threshold shifts were monitored in the guinea pigs before blast exposure and after gene transfer. Changes in cochlear morphology were examined with immunohistochemical and HE staining. Results One week after successful inoculation of hNGFβ, it was noted that Ad-hNGFβ protein was expressed in each turn of the cochlea, and its intensity was almost equal. After gene transfer in the combination group and the gene group, ABR threshold shifts at week 1 and 4 were significantly smaller than those of the APF group. HE staining showed that the number of the spiral ganglion cells in the combination group and the Ad-hNGFβ group was significantly higher than that of the control group after 4 weeks (P<0.01). Conclusions Ad-hNGFβ combined with iron fortified nutritional diet was effective in the prevention and treatment of blast hearing damage of cochlear hair cells.