额、枕部直接冲击导致中脑损伤的力学机制研究

目的分析额、枕部直接冲击造成中脑应力响应,探讨颅脑直接冲击导致脑干损伤的力学机制。方法构建头颅三维有限元模型,模拟计算额、枕部直接冲击损伤,分析中脑上、下交界面节点应力分布,总结中脑应力响应。结果中脑-间脑界面在冲击初始阶段,2ms即出现应力增高,中脑-桥脑界面应力升高开始于冲击后期(10ms),两交界面节点应力在冲击时程中均持续升高,额部冲击比枕部冲击在中-间脑界面造成的应力峰值高。结论中脑在颅脑直接冲击伤的致伤机制中占有突出地位,中脑的应力改变可解释脑震荡的意识障碍及部分原发脑干伤的临床表现。     

内眦赘皮与重睑成形同期手术的一种新方法

目的 探索适合同时行重睑成形术和内眦赘皮矫正术的新方法。方法 根据内眦赘皮深部眼轮匝肌结构上的异常特点，遵循内眦赘皮的上睑（倒向性内眦赘皮除外）内侧睑缘处皮肤短缺和尽量减少局部皮肤瘢痕的原则，设计适合同时行重睑成形术的新内眦赘皮矫正术（反Stallard法）。结果 临床应用86例，进行了为期6个月以上的随访，结果所有患者的重睑为稳定性平行型重睑，内眦已完全或大部分开大，手术效果满意。结论 此内眦赘皮矫正术能够充分矫正内眦部结构上的异常，而且适合与重睑成形术同期施行，是一种效果稳定，结果满意的术式。     

Synthesis of CCK-8 Tetrapeptide Fragment by Enzymatic Method

Cholecystokinin (CCK )isa polypeptidehor monecontaining 33aminoacidsresiduewithgastroin testinal,biliarandbrainactivities Itiswidelydis tributedinthevertebratecentralnervoussystem ,anditcanstimulateextensivelytheliberationofinsulinandglucagonfromtheLangerhansislets GreateffortshavebeendevotedtothesynthesisofCCK 8,itsderivativesandanalogues Amongthedifferentmethods ,theenzymaticmethodisthemosteffectiveonewithmanyadvantagesoverconventionalchemicalmethodologies :enzymespecificitysuppress essid…     

间接测热法和反向Fick氏法测定心脏手术病人术后氧耗量的比较

目的 采用反向Fick氏法和间接测热法测定心脏手术后病人的全身氧耗,并比较两种测量方法的相关性和准确性。方法8例心脏手术术后病人,分别在入ICU后2h和6h时同时采用反向Fick氏和间接测热法测定病人的全身氧耗量。结果 间接测热法和反向Fick法测得氧耗分别是(16±30)ml·min-1·m-2和(127±23)ml·min-1·m-2,前者的测定结果显著高于后者(P<0.01)。相关分析显示两者有较好的相关性(r=0.92,P<0.01)。采用Bland和Altman统计分析提示两种测定结果的平均偏离值为(35.5±13.4)ml·min-1·m-2,其95％的分布范围是(9～62)ml·min-1·m-2,提示两种测量结果间的一致性较差。结论 两种方法测定心脏病人术后全身氧耗的结果有明显差异,其中反向Fick氏法的测定结果误差大、准确性差,而间接测热法是较好的临床选择。     

52例电烧伤的治疗分析

目的：总结电烧伤的治疗方法。方法：对52例资料齐全的电烧伤病人的治疗方法及疗效进行回顾性分析。结果：单纯电火花烧伤的治疗和一般烧伤相同，电击伤多为早期清创，植皮覆盖创面。结论：电烧伤创面根据情况可有多种治疗方法：包括单纯换药，湿润烧伤膏，中厚皮片移植，皮瓣移植等。     

Using Computer Simulated Results of a Bulk Drug Substance Assay to Determine Acceptance Criteria for Method Validation

Purpose. To determine the statistical variability expected for a well designed HPLC assay of a bulk drug substance (BDS). The results are used to develop appropriate acceptance criteria for a method validation protocol as well as to evaluate the level of uncertainty expected for assay results using a variety of sampling/injection schemes. Methods. Computer simulation was used to generate a large quantity of data and the variability of the mock results was evaluated. Error propagation was also calculated, whenever possible, to confirm results obtained from the simulations. Results. Protocol acceptance criteria were developed that were consistent with the expected variability for data resulting from the execution of the validation protocol. In certain cases simulations provided the only avenue of obtaining results that could not otherwise be readily determined. Conclusions. Computer modeling can be used to obtain suitable acceptance criteria for validation results which are consistent with method variability. This is particularly significant in the case of linearity where it has been difficult to develop acceptance criteria based on anything other than analyst intuition and experience. Assay simulations clearly demonstrated that the variability expected for a typical BDS assay is large relative to the average specification range and therefore little insight about relative purity can be gained comparing individual passing assay results.     

Improved sib-pair linkage test for disease susceptibility loci

An improved sib-pair test for linkage is introduced which is superior to the previously proposed tests. The test is derived from the standard chi-squared goodness of fit statistic by restricting the alternative hypothesis to the genetically possible. Critical values are given and exact power comparisons are made with the previously proposed tests. The new test is shown to be more powerful for finite samples as well as being asymptotically uniformly most powerful. © 1993 Wiley-Liss, Inc.     

孔边裂纹J积分失效评定曲线

计算了单边椭圆缺口试件（ＳＥＮＣＰ）高应变区裂纹的Ｊ积分失效评定曲线（ＦＡＣ），考察了影响ＦＡＣ的几种因素。结果表明：在外加载荷与塑性屈服载荷之比（Ｌｒ）１．０时，用Ｒ６通用失效评定曲线进行结构高应变区裂纹断裂评定与疲劳剩余寿命估算是安全的。     

Exteroceptive suppression periods in jaw-closing muscles. Variability and relation to experimental pain and sustained muscle contraction

The duration of the late exteroceptive suppression period (ES₂) of temporal muscle EMG activity has been reported to be reduced in patients suffering from chronic tension-type headache. Methods of recording and analysing ES₂ have varied between centers and reproducibility of results within subjects , although insufficiently studied, has generally been poor. ES₂ was investigated in 30 healthy subjects, using a computerized technique of recording, rectifying and averaging the EMG signals. Hour to hour and week to week variations of ES₂ durations were calculated, and the influence of pain during a cold pressor test and of sustained muscle contraction on ES₂ durations was investigated. The intra-individual variation of ES₂ durations was 16.0% from hour to hour and 20.7% from week to week. The inter-individual variation was 36.7%. The present method for analysis of ES₂ periods proved to be reliable, as the intra-observer variation was 4.2% and the inter-observer variation 4.6%. ES₂ periods were significantly shorter on the first compared to the second day of examination ( p = 0.006) and during experimental pain ( p = 0.0005). We recommend the use of the computerized averaging technique in future studies and caution against the dependence of results upon factors such as conditioning and pain.     

pH指示剂吸收度比值法测定盐酸乙胺丁醇的含量

本文报道pH指示剂吸收度比值法测定盐酸乙胺丁醇的含量。该法采用指示剂为0.05%茜素黄R,测定波长374nm和500nm;标准液为0.1mol/L NaOH;仪器为WFZ-800D_2型分光光度计。测得三批盐酸乙胺丁醇的含量分别为99.5±0.04%,99.4±0.05%和99.6±0.03%.同时与药典法比较,结果一致。