广西毛南族农村妇女铁营养状况调查

对450名广西环江县毛南族农村育龄妇女进行血清铁蛋白(SF)、红细胞游离原卟啉(FEP)及血红蛋白(Hb)3项铁指标测定.结果发现:铁缺乏症患病率为27.8%(其中ID4.2%,IDE 7.8%,IDA 15.8%).随着孕次或产次的增加,血红蛋白水平逐渐下降,铁缺乏症患病率逐渐增高.有妊娠或分娩史的妇女与未孕或未生育的妇女血红蛋白水平与铁缺乏症患病率比较均有显著性差异(P<0.05).     

Is there a ‘Basque’ profile regarding autosomal recessive deficiencies of coagulation factors?*

When excluding haemophilia and von Willebrand disease, coagulation factors deficiencies constitute rare autosomal recessive disorders (<1 in 500,000) of less precisely defined epidemiology. We have reported herein the distribution of these entities in the French Basque Country, a genetic isolate of very old individualization with peculiar biological specificities. The prevalence of these disorders was markedly high, especially, as already shown, factor XI deficiency. This unusual profile needs to be discussed in the view of population genetics.     

碘缺乏病区碘盐防治后出生儿童头发中微量元素测定

对贵州碘缺乏病（ＩＤＤ）轻、重病区，碘盐防治后出生的轻度低智及正常智力儿童和非病区对照组儿童头发中硒（Ｓｅ）、锌（Ｚｎ）、铜（Ｃｕ）、铁（Ｆｅ）进行测定。结果显示轻病区儿童发Ｓｅ、Ｚｎ、Ｃｕ量及重病区儿童发Ｃｕ量显著低于非病区对照组。但是在轻、重病区内正常智力与轻度低智儿童头发中Ｓｅ、Ｚｎ、Ｃｕ、Ｆｅ量分别比较都没有显著差异。提示病区轻度低智儿童的发生与发中Ｓｅ、Ｚｎ、Ｃｕ、Ｆｅ的变化关系不密切。     

原子吸收光谱法测定糖尿病患者血清锌、镁、钙含量的研究

采用原子吸收光谱法测定了30例糖尿病患者血清锌、镁、钙的含量,并与30例正常人相比较。结果表明,其锌和镁较正常人为低(P<0.05),但钙与正常人无显著性差别。另外,本文还对锌、镁与糖尿病的关系进行了探讨。     

经咽旁入路选择性切除大鼠垂体前叶的研究

目的研究经咽旁入路选择性的切除大鼠垂体前叶，以制备大鼠垂体前叶激素缺乏模型如生长激素（GH）缺乏模型。方法经腹侧咽旁入路，运用显微神经外科技术经基蝶骨底选择性地切除垂体前叶。然后采用竞争免疫沉淀法检测大鼠生长激素的含量，术后统计死亡率、成功率。结果切除垂体前叶后大鼠的死亡率为26．7％，成活率为73．3％，全切率为91％；成功制备大鼠模型的GH显著低于假手术组和对照组。结论运用显微外科技术可以成功的选择性的切除大鼠垂体前叶，制备出垂体前叶激素如生长激素缺乏的动物模型。     

Challenges in the Diagnosis of the Right Patient for Testosterone Replacement Therapy

Diagnosis of testosterone deficiency is important to identify patients who might benefit from testosterone replacement therapy. Unfortunately, the diagnosis of hypogonadism may be a challenge for many practicing physicians, including endocrinologists and urologists. Signs and symptoms, such as sexual dysfunction, change in body composition, lethargy, and mood changes, are nonspecific and the available questionnaires are generally not useful in clinical practice. The diagnosis of testosterone deficiency is ultimately based on measurement of serum testosterone levels. However, marked variations in the reference ranges of serum testosterone levels among laboratories pose a challenge for physicians when interpreting the results. In addition, initial laboratory assessments usually determine total testosterone levels. About 1–2% of total testosterone is free and a further 30–50% is bound with low affinity to albumin; only these two components are bioavailable to the target tissues. In general, assuming the normal reference range for serum total testosterone in adult men is 300–1000 ng/dl (10–35 nmol/l), levels of < 250 ng/dl (8.7 nmol/l) suggest the patient is likely to be hypogonadal, whereas levels of > 350 ng/dl (12.7 nmol/l) suggest the symptoms may not be due to androgen deficiency. Values between 250 to 350 ng/dl warrant a repeat morning serum testosterone determination with assessment of free or bioavailable testosterone. In men with symptoms suggestive of androgen deficiency and borderline serum testosterone levels, where there are no contraindications to androgen therapy, a short therapeutic trial of testosterone may be justified.     

The profibrinolytic effect of plasma thrombomodulin in factor XI deficiency and its implications in hemostasis

Bleeding tendency in factor (F)XI deficiency may result from premature clot lysis due to insufficient thrombin activatable fibrinolysis inhibitor (TAFI) activation. Thrombomodulin (TM), upon binding to thrombin, is capable of modulating TAFI activation. In this study, we investigated the effects of plasma TM on fibrinolysis in FXI-deficient patients. A clot lysis assay showed the defective down-regulation of fibrinolysis in FXI-deficient patients as compared with normal controls. To evaluate the effects of plasma TM on fibrinolysis, a monoclonal anti-TM IgG was preincubated with plasma for 30 min. The presence of anti-TM IgG significantly prolonged the clot lysis times both in the FXI-deficient and normal plasma, indicating that plasma TM stimulated fibrinolysis. Furthermore, the presence of anti-TM IgG not only reduced protein C activation, but also increased thrombin generation and TAFI activation. The profibrinolytic effect of plasma TM was inhibited in the assay by including either a monoclonal anti-TAFI IgG or a specific TAFI inhibitor--carboxypeptidase inhibitor (CPI). Our results indicate that the impaired thrombin generation in FXI-deficient patients leads to the defective down-regulation of fibrinolysis, and that plasma TM stimulates fibrinolysis through APC pathway which inhibits TAFI activation. The profibrinolytic effect of plasma TM may contribute to the bleeding tendency observed in some FXI-deficient patients.     

富锌姬松茸胞内多糖的分离及体内抑制小鼠肝癌的研究

研究了经DEAE-纤维素离子交换柱分离的富锌液态发酵姬松茸胞内多糖组分对小鼠肝癌实体型Heps的体内抑制效果,同时考察了柱层析过后各组分中锌元素的分布情况及给药后小鼠血清锌的变化情况。分离所得组分MP-2在5 mg/(kg.d)剂量下抑瘤率为59.5%(p<0.001),MP-3在10 mg/(kg.d)剂量下抑瘤率为68.1%(p<0.001),两样品在10 mg/(kg.d)剂量下均能使小鼠脾指数显著提高。组分MP-2含锌率3.38‰、MP-3含锌率2.46‰,远高于组分MP-1的含锌率0.12‰;注射样品MP-2、MP-3的实验组小鼠血清锌浓度皆显著高于不给予富锌样品的阴性对照组(p<0.05)。