丹参超微饮片水溶性有效部位的HPLC指纹图谱研究

目的:采用高效液相色谱法(HPLC)对丹参超微饮片水溶性有效部位进行指纹图谱研究。方法:采用Hypersil BDS-C18柱(4.6mm×250mm,5μm),乙腈-0.014(体积分数)磷酸溶液梯度程序洗脱,流速1.0mL/min,柱温25℃,检测波长280nm。以27个共有峰为评价指标,使用“中药色谱指纹图谱相似度评价系统2004A版”软件计算处理。结果:建立的指纹图谱测定系统,对丹参水溶性成分分离良好。将8个产地的10批药材的指纹图谱进行相似度评价,相似系数均在0.9以上。结论:该法操作简便,精密度、稳定性和重复性良好,为丹参超微饮片水溶性有效部位的质量控制提供了方法。     

碘水剂造影在急腹症的应用(附122例分析)

本文报告122例急腹症使用碘水剂造影进行了检查,其中胃肠出血36例,穿孔11例,完全及不完全梗阻74例。95%经过造影明确了诊断,病变的部位及性质,特别是对胃肠道出血、穿孔、梗阻有很大的优越性。并讨论了适应症及禁忌症。     

尼美舒利固体分散体的非pH依赖性释放研究

目的以尼美舒利为难溶弱酸性模型药物,研究提高该类药物释放速率的方法。方法以聚乙二醇6000(PEG6000)为载体,采用熔融法制备尼美舒利固体分散体;测定含不同碱化剂(包括NaOH、KOH、Ca(OH)2、Na2CO3、CaCO3)的尼美舒利固体分散体中药物的释放速率。结果加入碱化剂能显著增加尼美舒利在蒸馏水中的释放度,碱化剂不同,药物的释放度不同;碱化剂的碱性越强,分散体的颜色越深,其吸湿性也相对越大。结论在尼美舒利PEG6000固体分散体中加入碱化剂可显著改善该类药物的体外释放特点,并呈现明显的非pH依赖性。     

乌拉尔甘草水溶性成分的HPLC指纹图谱研究

目的 建立乌拉尔甘草水溶性成分的HPLC指纹图谱,为有效控制乌拉尔甘草药材及甘草浸膏的质量奠定基础.方法 采用RP-HPLC法,色谱柱为Waters Sunfire~(TM) C_(18)(4.6 mm×150 mm,5 μm),以甲醇-0.1%磷酸水为流动相进行梯度洗脱,流速1.0 mL/min,检测波长260 nm,柱温25℃,检测时间95 min,分析了乌拉尔甘草主产区的12批样品.结果 在选定的色谱条件下,通过相似度分析确定18个色谱峰构成了乌拉尔甘草水溶性成分指纹图谱的特征峰,对其中的4个色谱峰进行了指认.结论 建立的乌拉尔甘草水溶性成分指纹图谱具有简便、稳定、可靠的优势,为乌拉尔甘草药材有效物质研究及甘草浸膏的质量控制奠定了基础.     

Enhanced oral bioavailability of dexibuprofen by a novel solid Self-emulsifying drug delivery system (SEDDS)

The main objective of this study was to prepare a solid form of lipid-based self-emulsifying drug delivery system (SEDDS) by spray drying liquid SEDDS with an inert solid carrier Aerosil 200 to improve the oral bioavailability of poorly water-soluble drug dexibuprofen. The liquid SEDDS was a system that consisted of dexibuprofen, Labrasol, Capryol 90 and Labrafil M 1944 CS. The particle size analysis revealed no difference in the z-average particle diameter of the reconstituted emulsion between liquid and solid SEDDS. The solid SEDDS was characterized by SEM, DSC and XRD studies. In vivo results of solid SEDDS and dexibuprofen powder in rats at the dose of 10 mg/kg showed that the initial plasma concentrations of drug in solid SEDDS were significantly higher than those of dexibuprofen powder (P < 0.05). The solid SEDDS gave significantly higher AUC and Cmax than did dexibuprofen powder (P < 0.05). In particular, the AUC of solid SEDDS was about twofold higher than that of dexibuprofen powder. Our results suggested that this solid SEDDS could be used as an effective oral solid dosage form to improve the bioavailability of poorly water-soluble drug dexibuprofen.     

怀地黄与山西晋南地黄的鉴别与比较

目的：对怀地黄与山西晋南地黄进行比较和鉴别。方法：采用性状鉴别、显微鉴别、浸出物含量测定等方法。对怀地黄与山西晋南地黄进行比较和鉴别。结果：怀地黄与山西晋南地黄在性状、显微特征、浸出物含量方面均存在一定差异,属不同品种。结论：应加强对不同产区中药材的质量控制。     

仿刺参水溶性海参皂苷的分离纯化及其抑瘤活性研究

为了获得具有显著抑瘤活性的水溶性海参皂苷样品, 利用大孔树脂柱、硅胶柱和葡聚糖凝胶柱对水溶性海参皂苷进行了分离纯化, 并对其体内外抑瘤活性和诱导肿瘤细胞凋亡进行了检测。研究结果显示, 大孔树脂柱的70%乙醇洗脱组分以及硅胶柱色谱的部分纯化组分 (pSC-2和pSC-3) 对HeLa细胞、A-549肺癌细胞、SGC-7901胃癌细胞和Bel-7402肝癌细胞均具有显著的抑瘤活性; 经过葡聚糖凝胶柱色谱, 获得了纯度均在99.6%以上、具有三萜类皂苷性质的SC-2和SC-3纯化样品, 发现SC-2纯化样品不仅能浓度和时间依赖性地抑制HeLa细胞的体外增殖, 还能剂量依赖性地抑制S180实体瘤的体内生长, 提高荷瘤小鼠的胸腺指数和脾指数。细胞凋亡检测结果显示, SC-2纯化样品可剂量依赖性地诱导HeLa细胞凋亡, SC-2纯化样品 (10和50 mg·L^-1) 处理12 h的HeLa细胞均出现了DNA片段化。可见, 水溶性海参皂苷SC-2纯化样品具有显著的抑瘤活性, 其机制可能是通过诱导肿瘤细胞发生凋亡来实现的。     

Propolis and the immune system: a review

Propolis has been used empirically for centuries and it was always mentioned as an immunomodulatory agent. In recent years, in vitro and in vivo assays provided new information concerning its mechanisms of action, thus a review dealing with propolis and the immune system became imperative. This review compiles data from our laboratory as well as from other researchers, focusing on its chemical composition and botanical sources, the seasonal effect on its composition and biological properties, its immunomodulatory and antitumor properties, considering its effects on antibody production and on different cells of the immune system, involving the innate and adaptive immune response. In vitro and in vivo assays demonstrated the modulatory action of propolis on murine peritoneal macrophages, increasing their microbicidal activity. Its stimulant action on the lytic activity of natural killer cells against tumor cells, and on antibody production was demonstrated. Propolis inhibitory effects on lymphoproliferation may be associated to its anti-inflammatory property. In immunological assays, the best results were observed when propolis was administered over a short-term to animals. Propolis antitumor property and its anticarcinogenic and antimutagenic potential are discussed. Since humans have used propolis for different purposes and propolis-containing products have been marketed, the knowledge of its properties with scientific basis is not only of academic interest but also of those who use propolis as well. This review opens a new perspective on the investigation of propolis biological properties, mainly with respect to the immune system.     

冠心病患者血清脂质过氧化水溶性荧光物质含量的变化及临床意义

检测２８例心绞痛、２８例急性心梗、１６例急性心梗恢复期患者及３８例健康人血清脂质过氧化水溶性荧光物质（ＷＳＦＳ）、血清脂质过氧化产物丙二醛（ＭＤＡ）和红细胞超氧化物歧化酶（ＳＯＤ）的水平。结果表明，冠心病各组ＷＳＦＳ显著高于对照组（Ｐ＜０．０５），而冠心病各组间ＷＳＦＳ无明显差异。冠心病患者ＷＳＦＳ与ＭＤＡ呈明显正相关（ｒ＝０．６０２，Ｐ＜０．０１），与ＳＯＤ呈明显负相关（ｒ＝－０．４２１，Ｐ＜０．０１）。血清ＷＳＦＳ可反映体内脂质过氧化水平，可作为临床诊断冠心病的参考指标。